PROCESS FOR CELL CULTURING BY CONTINUOUS PERFUSION AND ALTERNATING TANGENTIAL FLOW

• Main Authors: CROWLEY, JOHN, WUBBEN, MAIKE, COCO MARTIN, JOSE MANUEL
• Format: Patent
• Language: English
• Published: 17.03.2016
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Use of a process wherein a cell culture comprising cell culture medium and a suspension of cells is circulated over a filter module comprising hollow fibers wherein the flow within the filter module is an alternating tangential flow, for the reduction of the degree of aggregation of the cells in the...