Advanced pathogen detection and screening

• Main Authors: COLEMAN, ELIZABETH J, ICENHOUR, CRYSTAL, LOYAL, BRIAN V
• Format: Patent
• Language: English
• Published: 15.12.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Disclosed is a rapid, dual purpose, PCR-hased method for identifying two or more pathogens, including , in an extracted sample, such as stool or environmental (soil, water) isolates, in individual real-time PCR reactions. This method is of particular utility for clinical veterinary, and environmental Jesting applications. The present methods are more sensitive than conventional ELISA or JFA- based methods of detection An internal control (IC) for use in the PCR based nucleic acid detection method is. also disclosed.