Purification of recombinant human factor XIII

• Main Authors: TOPPING, ANDREW, O'DONNELL, RAY, CHEEMA, HARDARSHAN, MENG, WENMAO, HOGG, DEBORAH, BRANSFORD, CAROL, ROBERTSON, EWAN
• Format: Patent
• Language: English
• Published: 19.01.2012
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The present invention provides improved methods for the purification of factor XIII. In particular, the methods provide compositions containing 5 % or less contaminating proteins. In particular embodiments of the present invention the methods provide purified factor XIII compositions comprising less than 1 % activated factor XIII, less than 2 % protein aggregates, and/or less than 5 % charge isomers of factor XIII. The methods do not require the use a precipitation or crystallization step common to prior methods of isolating factor XIII. Instead, the method uses immobilized metal affinity chromatography to remove various contaminants common to recombinant expression of factor XIII. Further, a combination of various chromatography methods including ion exchange chromatography, hydrophobic affinity chromatography, and immobilized metal affinity chromatography comprise a simple and less expensive method to produce a pharmaceutical grade factor XIII product at high yield.