GnTIII (UDP-n-acethylglucosamine:beta-D mannoside beta (1,4)-N-acethylglucosaminy ltransferase III) expression in plants

• Main Authors: HENDRIKUS ANTONIUS CORNELUS BAKKER, HENDRIK JAN BOSCH, DIONISIUS ELISABETH ANTONIUS FLORACK
• Format: Patent
• Language: English
• Published: 08.05.2008
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; POLYSACCHARIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; THEIR PREPARATION OR CHEMICAL WORKING-UP; TRAPPING; VINEGAR; WINE

The present invention relates to a hybrid protein comprising an active site of mammalian UDP-N-acetylglucosamine: ²-D mannoside ²(1,4)-N-acetylglucosaminyltransferase (GnTIII) and a transmembrane region (Tm) of a protein that resides in the endoplasmic reticulum or Golgi apparatus of a eukaryotic cell.