NEUER MEGAKARYOKYTISCHER KOLONIESTIMULIERENDER FAKTOR UND VERFAHREN ZUR HERSTELLUNG

• Main Authors: MATSUNAGA, KEITA, OGATA, KIYOYUKI, MAKABE, OSAMU, KURIYA, SHINICHIRO, NOMURA, TAKEO, NAGAOKA, KOZO, HAMAGUCHI, HIROYUKI, NOJIRI, CHUHEI
• Format: Patent
• Language: German
• Published: 15.07.1997
• Edition: 6
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

According to this invention, a novel megakaryocytic colony stimulating factor is obtained by culturing a cell strain of human pulmonary large cell cancer, for example, PC-13 cell strain or human lung cancer cell strain MC-1 separated as monoclonal cells from PC-13 cell strain, recovering a supernatant of the culture liquor, collecting from the supernatant a megakaryocytic colony stimulating factor (Meg-CSF) comprising a protein having the activity of forming a megakaryocytic colony from myeloid cells when applied to human or mouse myeloid cells in vitro and the activity of increasing the number of platelets in the peripheral vessel in vivo, and purifying the factor. The obtained colony stimulating factor is a protein having a molecular weight of 24,000 and an isoelectric point of 4.5 to 5.5, and is useful for treating a certain kind of platelet reducing diseases. This factor can be mixed with conventional various excipients to form medicinal compositions in various preparation forms.