Antifibrotic effects of Punica granatum peels through stimulation of hepatic stellate cell apoptosis in thioacetamideinduced liver fibrosis in rats
Background/aim Liver fibrosis is a major global health problem. The present study aimed to evaluate the antioxidant and antifibrogenic potential of Punica granatum peels extract against thioacetamide (TAA)-induced hepatic fibrosis. Materials and methods Rats were divided into six groups. Group 1 was...
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Published in | Journal of the Arab Society for Medical Research Vol. 12; no. 2; pp. 56 - 67 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cairo, Egypt
Arab Society for Medical Research
2017
|
Online Access | Get full text |
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Abstract | Background/aim
Liver fibrosis is a major global health problem. The present study aimed to evaluate
the antioxidant and antifibrogenic potential of Punica granatum peels extract
against thioacetamide (TAA)-induced hepatic fibrosis.
Materials and methods
Rats were divided into six groups. Group 1 was the control; group 2 was injected
with TAA (150 mg/kg, intraperitoneal) (fibrosis group) for 4 weeks; group 3 received
P. granatum peels extract only (200 mg/kg); group 4 rats were given oral sliymarin
(50 mg/kg) for 4 weeks after withdrawal of TAA; groups 5 and 6 rats were given oral
P. granatum peels extract (100 and 200 mg/kg) for 4 weeks after withdrawal of TAA.
Fibrosis was assessed histologically and by measuring the hepatic hydroxyproline
content. The degree of liver fibrosis was assessed by Masson’s trichrome staining
and α-smooth muscle actin as the marker of the activated hepatic stellate cells was
detected immunohistochemically. Serum markers of liver damage and oxidative
stress were also assessed.
Results
The biochemical analyses have shown that P. granatum peels extract or sliymarin
significantly reduced the progression of hepatic fibrosis. The plant extract or
sliymarin resulted in a significant improvement of liver damage by the reduced
levels of serum alanine aminotransferase and alkaline phosphatase. Oral
administration of P. granatum peels or sliymarin has also restored normal levels
of malondialdehyde, hydroxyproline content as markers of fibrosis content
(P<0.05) in the liver, and retained control activities of endogenous antioxidants
such as superoxide dismutase, nitric oxide, and glutathione. The histological
evaluation showed that the plant extract or silymarin treatment maintained the
architecture of the liver nearly normal and attenuate the accumulation of excessive
collagen in the liver fibrosis caused by TAA. We also observed that P. granatum
peels extract or silymarin-treated rats reduced α-smooth muscle actin.
Conclusion
The obtained results have shown that P. granatum peels extract effectively blocked
hepatic stellate cell proliferation and they may be beneficial in the treatment of liver
fibrosis. |
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AbstractList | Background/aim
Liver fibrosis is a major global health problem. The present study aimed to evaluate
the antioxidant and antifibrogenic potential of Punica granatum peels extract
against thioacetamide (TAA)-induced hepatic fibrosis.
Materials and methods
Rats were divided into six groups. Group 1 was the control; group 2 was injected
with TAA (150 mg/kg, intraperitoneal) (fibrosis group) for 4 weeks; group 3 received
P. granatum peels extract only (200 mg/kg); group 4 rats were given oral sliymarin
(50 mg/kg) for 4 weeks after withdrawal of TAA; groups 5 and 6 rats were given oral
P. granatum peels extract (100 and 200 mg/kg) for 4 weeks after withdrawal of TAA.
Fibrosis was assessed histologically and by measuring the hepatic hydroxyproline
content. The degree of liver fibrosis was assessed by Masson’s trichrome staining
and α-smooth muscle actin as the marker of the activated hepatic stellate cells was
detected immunohistochemically. Serum markers of liver damage and oxidative
stress were also assessed.
Results
The biochemical analyses have shown that P. granatum peels extract or sliymarin
significantly reduced the progression of hepatic fibrosis. The plant extract or
sliymarin resulted in a significant improvement of liver damage by the reduced
levels of serum alanine aminotransferase and alkaline phosphatase. Oral
administration of P. granatum peels or sliymarin has also restored normal levels
of malondialdehyde, hydroxyproline content as markers of fibrosis content
(P<0.05) in the liver, and retained control activities of endogenous antioxidants
such as superoxide dismutase, nitric oxide, and glutathione. The histological
evaluation showed that the plant extract or silymarin treatment maintained the
architecture of the liver nearly normal and attenuate the accumulation of excessive
collagen in the liver fibrosis caused by TAA. We also observed that P. granatum
peels extract or silymarin-treated rats reduced α-smooth muscle actin.
Conclusion
The obtained results have shown that P. granatum peels extract effectively blocked
hepatic stellate cell proliferation and they may be beneficial in the treatment of liver
fibrosis. |
Author | Umara, Inayat A. Jalal, Asma F. Farraj, Abd al-Raziq H. Nada, Sumayyah A. al-Tumi, Sayyid A. Sharaf, Hafizah Ali Hasan, Nabilah S. |
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Snippet | Background/aim
Liver fibrosis is a major global health problem. The present study aimed to evaluate
the antioxidant and antifibrogenic potential of Punica... |
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Title | Antifibrotic effects of Punica granatum peels through stimulation of hepatic stellate cell apoptosis in thioacetamideinduced liver fibrosis in rats |
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