Nitrate Binding to Limulus polyphemusSubunit Type II Hemocyanin and its Functional Implications

• Published in: Journal of molecular biology Vol. 262; no. 4; pp. 532 - 542
• Main Authors: Hazes, Bart, Magnus, Karen A., Kalk, Kor H., Bonaventura, Celia, Hol, Wim G.J.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 1996
• Subjects: allosteric regulation; Bohr effect; crystal structure; hemocyanin; nitrate binding; Bohr effect; nitrate binding; hemocyanin; crystal structure; allosteric regulation

The horseshoe crab, Limulus polyphemus, employs hemocyanin as an oxygen carrier in its hemolymph. This hemocyanin displays cooperative oxygen binding and heterotropic allosteric regulation by protons, chloride ions and divalent cations. Here, we report the crystal structure of Limulus polyphemussubu...