Central T cell tolerance: Identification of tissue-restricted autoantigens in the thymus HLA-DR peptidome
Abstract Promiscuous gene expression (pGE) of tissue-restricted self-antigens (TRA) in medullary thymic epithelial cells (mTECs) is in part driven by the Autoimmune Regulator gene ( AIRE ) and essential for self-tolerance. The link between AIRE functional mutations and multi-organ autoimmunity in hu...
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Published in | Journal of autoimmunity Vol. 60; pp. 12 - 19 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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2015
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Abstract | Abstract Promiscuous gene expression (pGE) of tissue-restricted self-antigens (TRA) in medullary thymic epithelial cells (mTECs) is in part driven by the Autoimmune Regulator gene ( AIRE ) and essential for self-tolerance. The link between AIRE functional mutations and multi-organ autoimmunity in human and mouse supports the role of pGE. Deep sequencing of the transcriptome revealed that mouse mTECs potentially transcribe an unprecedented range of >90% of all genes. Yet, it remains unclear to which extent these low-level transcripts are actually translated into proteins, processed and presented by thymic APCs to induce tolerance. To address this, we analyzed the HLA-DR-associated thymus peptidome. Within a large panel of peptides from abundant proteins, two TRA peptides were identified: prostate-specific semenogelin-1 (an autoantigen in autoimmune chronic prostatitis/chronic pelvic pain syndrome) and central nervous system-specific contactin-2 (an autoantigen in multiple sclerosis). Thymus expression of both genes was restricted to mTECs. SEMG1 expression was confined to mature HLA-DRhi mTECs of male and female donors and was AIRE-dependent, whereas CNTN2 was apparently AIRE-independent and was expressed by both populations of mTECs. Our findings establish a link between pGE, MHC-II peptide presentation and autoimmunity for bona fide human TRAs. |
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AbstractList | Abstract Promiscuous gene expression (pGE) of tissue-restricted self-antigens (TRA) in medullary thymic epithelial cells (mTECs) is in part driven by the Autoimmune Regulator gene ( AIRE ) and essential for self-tolerance. The link between AIRE functional mutations and multi-organ autoimmunity in human and mouse supports the role of pGE. Deep sequencing of the transcriptome revealed that mouse mTECs potentially transcribe an unprecedented range of >90% of all genes. Yet, it remains unclear to which extent these low-level transcripts are actually translated into proteins, processed and presented by thymic APCs to induce tolerance. To address this, we analyzed the HLA-DR-associated thymus peptidome. Within a large panel of peptides from abundant proteins, two TRA peptides were identified: prostate-specific semenogelin-1 (an autoantigen in autoimmune chronic prostatitis/chronic pelvic pain syndrome) and central nervous system-specific contactin-2 (an autoantigen in multiple sclerosis). Thymus expression of both genes was restricted to mTECs. SEMG1 expression was confined to mature HLA-DRhi mTECs of male and female donors and was AIRE-dependent, whereas CNTN2 was apparently AIRE-independent and was expressed by both populations of mTECs. Our findings establish a link between pGE, MHC-II peptide presentation and autoimmunity for bona fide human TRAs. |
Author | Collado, Javier A James, Eddie A Kwok, William W Canals, Françesc Alvarez, Iñaki Gärtner, Martina Kyewski, Bruno Carrascal, Montserrat Jaraquemada, Dolores Ciudad, M. Teresa Pujol-Borrell, Ricardo Colobran, Roger |
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Keywords | TCR TRA promiscuous gene expression Xcorr Peptides AIRE M-MLV RT SVS2 liquid chromatography/mass spectrometry MHC major histocompatibility complex TFA Thymus pGE autoimmune polyendocrinopathy candidiasis ectodermal dystrophy APS-1 FDR cross correlation score mTEC false discovery rate APECED gDNA PSA Human genomic DNA T cell receptor cycle threshold Tolerance tissue-restricted antigen Autoantigens autoimmune regulator autoimmune polyendocrine syndrome type 1 LC/MS CT seminal vesicle secretory protein 2 medullary thymic epithelial cell trifluoroacetic acid APC Moloney murine leukemia virus reverse transcriptase antigen presenting cell dendritic cell DC prostate-specific antigen |
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Snippet | Abstract Promiscuous gene expression (pGE) of tissue-restricted self-antigens (TRA) in medullary thymic epithelial cells (mTECs) is in part driven by the... |
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Title | Central T cell tolerance: Identification of tissue-restricted autoantigens in the thymus HLA-DR peptidome |
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