Partial sequence of bop gene for Haloterrigena thermotolerans JCM 1I050T(嗜盐古菌Haloterrigena thermotolerans JCM 11050Tbop基因序列研究)

对标准菌株Haloterrigena ihermotolerans JCM 11050T，其编码螺旋C至螺旋G的细菌视紫红质(bacteriorhodopsin, BR)蛋白基因（bop基因）片断采用PCR方法进行了扩增，TA克隆并测定了其核苷酸序列.对翻译出的氨基酸序列进行亲水性分析，表明该菌株的BR蛋白与已报道相应序列具有类似的二级结构.蛋白序列聚合比对结果表明，该菌株BR蛋白与Natrinema属的BR蛋白具有一定相似性.在这一Haloterrigena属的标准菌株中证实bop基因，进一步丰富了bop基因资源....

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Published inZhejiang da xue xue bao. Journal of Zhejiang University. Sciences edition. Li xue ban Vol. 34; no. 5; pp. 576 - 579
Main Authors LIYong-hua(李永华), JINWen-tao(金文涛), HUOYing-yi(霍颖异), WUMin(吴敏)
Format Journal Article
LanguageChinese
Published Zhejiang University Press 01.09.2007
Subjects
嗜盐古菌
系统发育
细菌视紫红质
Online AccessGet full text
ISSN1008-9497

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Abstract 对标准菌株Haloterrigena ihermotolerans JCM 11050T，其编码螺旋C至螺旋G的细菌视紫红质(bacteriorhodopsin, BR)蛋白基因（bop基因）片断采用PCR方法进行了扩增，TA克隆并测定了其核苷酸序列.对翻译出的氨基酸序列进行亲水性分析，表明该菌株的BR蛋白与已报道相应序列具有类似的二级结构.蛋白序列聚合比对结果表明，该菌株BR蛋白与Natrinema属的BR蛋白具有一定相似性.在这一Haloterrigena属的标准菌株中证实bop基因，进一步丰富了bop基因资源.
AbstractList 对标准菌株Haloterrigena ihermotolerans JCM 11050T，其编码螺旋C至螺旋G的细菌视紫红质(bacteriorhodopsin, BR)蛋白基因（bop基因）片断采用PCR方法进行了扩增，TA克隆并测定了其核苷酸序列.对翻译出的氨基酸序列进行亲水性分析，表明该菌株的BR蛋白与已报道相应序列具有类似的二级结构.蛋白序列聚合比对结果表明，该菌株BR蛋白与Natrinema属的BR蛋白具有一定相似性.在这一Haloterrigena属的标准菌株中证实bop基因，进一步丰富了bop基因资源.
Author WUMin(吴敏)
JINWen-tao(金文涛)
LIYong-hua(李永华)
HUOYing-yi(霍颖异)
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  fullname: JINWen-tao(金文涛)
  organization: College of Life Science, Zhejiang University, Hangzhou 310027, China(浙江大学生命科学学院，浙江　杭州　310027)
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  fullname: HUOYing-yi(霍颖异)
  organization: College of Life Science, Zhejiang University, Hangzhou 310027, China(浙江大学生命科学学院，浙江　杭州　310027)
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  fullname: WUMin(吴敏)
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Snippet 对标准菌株Haloterrigena ihermotolerans JCM 11050T，其编码螺旋C至螺旋G的细菌视紫红质(bacteriorhodopsin,...
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StartPage 576
SubjectTerms 嗜盐古菌
系统发育
细菌视紫红质
Title Partial sequence of bop gene for Haloterrigena thermotolerans JCM 1I050T(嗜盐古菌Haloterrigena thermotolerans JCM 11050Tbop基因序列研究)
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