Fermentation Conditions Optimization and Components Analysis of Antimicrobial Substances-producing Endophytic Fungus CWJ01 from Acanthopanax senticosus

• Published in: Shipin gongye ke-ji Vol. 45; no. 17; pp. 209 - 220
• Main Authors: Yuguo LI, Kun JIANG, Daozhi ZHANG, Hengwei XU, Danping FENG, Xiaoqian MENG, Chunying ZHENG
• Format: Journal Article
• Language: Chinese
• Published: The editorial department of Science and Technology of Food Industry 01.09.2024
• Subjects: acanthopanax senticosus; bacteriostatic activity; endophytic fungus; fermentation condition; secondary metabolites; strain identification
• DOI: 10.13386/j.issn1002-0306.2023110328
• ISSN: 1002-0306

An endophytic fungus CWJ01 with good antibacterial activity was isolated from the leaves of Acanthopanax senticosus. In order to improve the antibacterial activity of the fermentation broth of this strain and explore the substance basis of its antibacterial activity, the fermentation conditions of the endophytic fungus CWJ01 were optimized by orthogonal experiment according the antibacterial activity on the basis of single factor experiments. The secondary metabolites contained in the endophytic fungus CWJ01 were separated by column chromatography. The strains of the endophytic fungus CWJ01 were identified by morphology and ITS sequence analysis. The results showed that the optimum fermentation conditions of CWJ01 were as follows: PDB as the base medium, 2% glucose as the carbon source, 1% peptone as the nitrogen source. The initial pH of the medium was 7.0, the liquid content of the medium was 60%, the inoculated strain was 5%, the rotating speed was 140 r/min, the temperature was 28 ℃, and the culture time