Tissue culture parameters in sweet orange cultivars Parâmetros de cultura de tecidos em cultivares de laranjeira-doce

• Published in: Pesquisa agropecuaria brasileira Vol. 43; no. 8; pp. 1093 - 1096
• Main Authors: Amanda Ferreira da Silva Mendes, Luciana Cardoso Cidade, Gabrielle Novais Manzoli, Wagner Campos Otoni, Walter dos Santos Soares Filho, Marcio Gilberto Cardoso Costa
• Format: Journal Article
• Language: English
• Published: Embrapa Informação Tecnológica 01.08.2008
• Subjects: biotechnology; biotecnologia; Citrus sinensis; gene transfer; morfogênese; morphogenesis; regeneration; regeneração; transferência gênica

The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in co...