Genetic analysis of weak expression of ABO blood group antigens in neonates

• Published in: Zhongguo shu xue za zhi Vol. 38; no. 1; pp. 85 - 90
• Main Authors: YANG Jiali, ZHAO Ding, LI Wei, ZHANG Xiaopan, LI Zhihao, TIAN Dongdong
• Format: Journal Article
• Language: Chinese
• Published: Institute of Blood Transfusion of Chinese Academy of Medical Sciences 01.01.2025
• Subjects: abo blood group; abo subtype; gene sequencing; neonate; weak expression of antigen
• ISSN: 1004-549X
• DOI: 10.13303/j.cjbt.issn.1004-549x.2025.01.014

[Objective] To perform genetic analysis on samples with weak agglutination and mixed agglutination of ABO blood group antigens in neonates, and to investigate the molecular biological characteristics of ABO subtypes in neonates. [Methods] Serological identification of ABO blood group was performed by tube method and microcolumn gel method. The ABO exons 2-7 were amplified by PCR, and the amplified products were sequenced by Sanger sequencing method to determine the genotype. [Results] Among the ABO blood group serological results of 14 neonates, 8 cases showed weakened A antigen, and 6 cases showed weakened B antigen. Seven samples were identified with ABO subtype alleles, with genotypes as A102/B101+c.538C>T, Aw26/B102, A205/O02, A205/B101(2 cases), Aw26/O02, B(A)06/O01, B101/O01(3 cases), A102/O01(2 cases), A102/B101 and B101/O02. Additionally, three other family members were also found to carry B(A)06 allele in a pedigree investigation. [Conclusion] For samples showing weakened antigens in ABO blood type i