Matrine alleviates paraquat-induced pulmonary fibrosis in mouse models

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 42; no. 9; pp. 1385 - 1390
• Main Author: LI Mei-qi, DENG Jun, WANG Song-ping
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.09.2022
• Subjects: pulmonary fibrosis| paraquat| matrine| epithelial-mesenchymal transition
• ISSN: 1001-6325
• DOI: 10.16352/j.issn.1001-6325.2022.09.1385

Objective To study the effect of matrine on paraquat(PQ)-induced pulmonary fibrosis in mice and its possible mechanism. Methods Mice were divided into the control group, the paraquat(PQ) group and the matrine group randomly. The pulmonary fibrosis model was induced by intraperitoneal injection of paraquat (18 mg/kg). Twenty-four hours after modeling, the treatment group was intragastrically administered with matrine (100 mg/kg). Twenty-eight days later, the lung tissues of each group were collected; HE staining and Masson staining were used to observe the severity of inflammation and fibrosis in the lung tissue; Ashcroft scale was used to evaluate pulmonary fibrosis, and the content of hydroxyproline(HYP) in lung tissue of mice was detected by alkaline water method; Western blot was used to detect the expression of collagen Ⅰ and collagen Ⅲ; The expression of epithelial-mesenchymal transition(EMT)-related proteins[ alpha-smooth muscle action(α-SMA), E-cardherin and vimentin] was detected by immunofluorescence