Performance of nested-RT-PCR assays for Zika and Dengue detection

• Published in: Acta scientiarum. Biological sciences Vol. 47; no. 1
• Main Authors: José Henrique Francisco Roma, Rachel Cruz Alves, Stefhano Luis Candido, Valéria Dutra, Luciano Nakazato, Renata Dezengrini-Slhessarenko, Juliana Helena Chavez-Pavoni, Mariângela Ribeiro Resende
• Format: Journal Article
• Language: English
• Published: Universidade Estadual de Maringá 01.08.2025
• Subjects: arboviruses; diagnosis; PCR, sensitivity and specificity

Arboviruses represent a public health problem in developing countries. Accurate diagnosis has been challenging in Dengue virus endemic countries, especially since the introduction of the Zika and Chikungunya viruses. The aim of this study was to determine the sensitivity and specificity of nested Re...