Effects of baicalein on protein expression of Th1/2 cells, obestatin and 5-hydroxytryptamine in rats with ulcerative

• Published in: Zhongguo lin chuang yan jiu Vol. 37; no. 6; pp. 859 - 900
• Main Authors: KUANG Zhe, GUO Huili, ZHANG Yongchao, MIAO Zhiwei
• Format: Journal Article
• Language: Chinese
• Published: The Editorial Department of Chinese Journal of Clinical Research 01.06.2024
• Subjects: 5-hydroxytryptamine; baicalein; brain-gut peptide; colon mucosa damage index; disease activity index; interferon; interleukin; obestatin; sulfasalazine; t helper cells; ulcerative colitis
• ISSN: 1674-8182
• DOI: 10.13429/j.cnki.cjcr.2024.06.016

Objective To investigate the effects of baicalein on T helper cells (Th) 1/2, obestatin and 5-hydroxytryptamine (5-HT) in rats with ulcerative colitis. Methods Forty SPF grade male SD rats were randomly divided into normal group (group N), model group (group M), sulfasalazine group (group S), and baicalein group (group B), with 10 rats in each group. The ulcerative colitis models were established in group M, S and B by trinitrobenzenesulfonic acid. After successful modeling, 0.3 g/kg sulfasalazine was given to group S by gavage, and 50 mg/kg baicalein was given to group B by gavage. Group N and group M were given the same volume of normal saline by gavage at the same time. The disease activity index (DAI) score and colon mucosa damage index (CMDI) score of rats were evaluated, and the pathological morphology of colon tissue was detected by hematoxylin and eosin staining. The protein expression of Th1 cytokine interferon-γ (IFN-γ) and Th2 cytokine interleukin-4 (IL-4) in colon tissues was detected by western b