Application of Flow Cytometry in Agricultural Research
Flow cytometry is a biological technique for counting and sorting out tiny particles suspended in fluid based on flow cytometer through the detection of scattering or coupled fluorescence signals to obtain a series of important biophysical and biochemical related characteristics of suspended particl...
Saved in:
Published in | Guangdong nong ye ke xue Vol. 49; no. 11; pp. 66 - 73 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Guangdong Academy of Agricultural Sciences
01.11.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Flow cytometry is a biological technique for counting and sorting out tiny particles suspended in fluid based on flow cytometer through the detection of scattering or coupled fluorescence signals to obtain a series of important biophysical and biochemical related characteristics of suspended particles (usually cells, bacteria and other tiny particles) in a quick, accurate, objective and high-throughput way, and to automatically analyze and sort out specific populations according to the pre-selected parameter range of cells, bacteria and other tiny particles. Flow cytometry is widely used in many scientific fields, especially biotechnology and medicine. Although the application of flow cytometry in agricultural research started relatively late, great progress has been made. With the continuous improvement of the performance of flow cytometer, the continuous development and improvement of labeling methods and detection technologies, the application prospect of flow cytometry will become wider, and it will also play an increasingly important role in the agricultural field. In this review, it introduces the working principle and functional classification of flow cytometer, discusses the application and research progress of flow cytometry in agricultural fields such as crop genome and protoplast analysis, stress resistance research, animal immunity and trace element analysis, sperm quality and sex control, toxin toxicity analysis, pathogenic bacteria and virus analysis, and then looks forward to the development prospect of this research field, providing a new direction for the potential application of flow cytometry in agricultural fields. |
---|---|
AbstractList | Flow cytometry is a biological technique for counting and sorting out tiny particles suspended in fluid based on flow cytometer through the detection of scattering or coupled fluorescence signals to obtain a series of important biophysical and biochemical related characteristics of suspended particles (usually cells, bacteria and other tiny particles) in a quick, accurate, objective and high-throughput way, and to automatically analyze and sort out specific populations according to the pre-selected parameter range of cells, bacteria and other tiny particles. Flow cytometry is widely used in many scientific fields, especially biotechnology and medicine. Although the application of flow cytometry in agricultural research started relatively late, great progress has been made. With the continuous improvement of the performance of flow cytometer, the continuous development and improvement of labeling methods and detection technologies, the application prospect of flow cytometry will become wider, and it will also play an increasingly important role in the agricultural field. In this review, it introduces the working principle and functional classification of flow cytometer, discusses the application and research progress of flow cytometry in agricultural fields such as crop genome and protoplast analysis, stress resistance research, animal immunity and trace element analysis, sperm quality and sex control, toxin toxicity analysis, pathogenic bacteria and virus analysis, and then looks forward to the development prospect of this research field, providing a new direction for the potential application of flow cytometry in agricultural fields. |
Author | Minying MO Jianling ZHANG Lei WANG Zixuan DENG Dizhou LUO Yinghui SU Yuanjie FANG Xiaoai ZHANG |
Author_xml | – sequence: 1 fullname: Lei WANG organization: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Guangzhou 510640, China – sequence: 2 fullname: Dizhou LUO organization: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Guangzhou 510640, China – sequence: 3 fullname: Zixuan DENG organization: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Guangzhou 510640, China – sequence: 4 fullname: Minying MO organization: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Guangzhou 510640, China – sequence: 5 fullname: Jianling ZHANG organization: Jiangmen Customs Technical Center, Jiangmen 529085, China – sequence: 6 fullname: Yinghui SU organization: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China – sequence: 7 fullname: Yuanjie FANG organization: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Guangzhou 510640, China – sequence: 8 fullname: Xiaoai ZHANG organization: Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Guangzhou 510640, China |
BookMark | eNqtjLFuwjAUAD1QqbTlH7ww4vrZwQkjQkVlRQzdrBfHoY6MHdlGKH8Pqqp-QW856YZ7IbMQgyVkCZyBqlXzPjCXc2DAebVq6uqLCS4EA2CcNzMy_-vPZJHzwB9sNlKsxZyo7Th6Z7C4GGjs6d7HG91NJV5sSRN1gW7PyZmrL9eEnh5ttpjM9xt56tFnu_j1KznsP067z1UXcdBjchdMk47o9E-I6awxFWe81WuJUrZcgAVTtS2g6VVXd0opqKATUv7n6w6yclnn |
ContentType | Journal Article |
DBID | DOA |
DOI | 10.16768/j.issn.1004-874X.2022.11.008 |
DatabaseName | DOAJ Directory of Open Access Journals |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Agriculture |
EndPage | 73 |
ExternalDocumentID | oai_doaj_org_article_53a33b021e1c4bb1acf6d7d666141d23 |
GroupedDBID | -04 5XA 5XE 92G 92I ABDBF ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CW9 GROUPED_DOAJ TCJ TGD U1G U5N |
ID | FETCH-doaj_primary_oai_doaj_org_article_53a33b021e1c4bb1acf6d7d666141d233 |
IEDL.DBID | DOA |
ISSN | 1004-874X |
IngestDate | Tue Oct 22 15:14:45 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 11 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-doaj_primary_oai_doaj_org_article_53a33b021e1c4bb1acf6d7d666141d233 |
OpenAccessLink | https://doaj.org/article/53a33b021e1c4bb1acf6d7d666141d23 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_53a33b021e1c4bb1acf6d7d666141d23 |
PublicationCentury | 2000 |
PublicationDate | 2022-11-01 |
PublicationDateYYYYMMDD | 2022-11-01 |
PublicationDate_xml | – month: 11 year: 2022 text: 2022-11-01 day: 01 |
PublicationDecade | 2020 |
PublicationTitle | Guangdong nong ye ke xue |
PublicationYear | 2022 |
Publisher | Guangdong Academy of Agricultural Sciences |
Publisher_xml | – name: Guangdong Academy of Agricultural Sciences |
SSID | ssj0000993252 |
Score | 4.508988 |
Snippet | Flow cytometry is a biological technique for counting and sorting out tiny particles suspended in fluid based on flow cytometer through the detection of... |
SourceID | doaj |
SourceType | Open Website |
StartPage | 66 |
SubjectTerms | agricultural microorganism agriculture animal husbandry crop flow cytometry |
Title | Application of Flow Cytometry in Agricultural Research |
URI | https://doaj.org/article/53a33b021e1c4bb1acf6d7d666141d23 |
Volume | 49 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEB5kBdGD-MTnkoMeu9s0D7vHdrWsgp4UeivJJhEXbaVUZP-9SVPd3jzoMQ8m4QuT-UImXwAuzEQIJjUPBKYmoCbSwcRIFagrJWwhZqpV179_4LMnepezvPfVl8sJ8_LAHrgxI4IQaSORxnMqJRZzw60h7uIKVpHX-cSsd5haeN5DIuavOkNqXZ7mG3Dpdghu-fV40XrY6KfNnhOjaOTUPN03kz3x_jbKZDuw3dFDlPhp7cKaLvdgK3muO4kMvQ88Wd05o8qg7LX6RNNlU73ppl6ilxKtultT36l1B3Cb3TxOZ4Ebtnj3IhOFk31uKywYRQdG8RsY5BAGZVXqI0BccKatsxJJOA2JiGNMJbX8IlREW454DOnfxzv5DyOnsOng9w_8zmDQ1B_63Eb6Rg5hPUmv02zYLu4Xugep4Q |
link.rule.ids | 315,786,790,870,2115,27955,27956 |
linkProvider | Directory of Open Access Journals |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Application+of+Flow+Cytometry+in+Agricultural+Research&rft.jtitle=Guangdong+nong+ye+ke+xue&rft.au=Lei+WANG&rft.au=Dizhou+LUO&rft.au=Zixuan+DENG&rft.au=Minying+MO&rft.date=2022-11-01&rft.pub=Guangdong+Academy+of+Agricultural+Sciences&rft.issn=1004-874X&rft.volume=49&rft.issue=11&rft.spage=66&rft.epage=73&rft_id=info:doi/10.16768%2Fj.issn.1004-874X.2022.11.008&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_53a33b021e1c4bb1acf6d7d666141d23 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1004-874X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1004-874X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1004-874X&client=summon |