Selection of Powdery Mildew Resistant Brassica Genotypes Based on Disease Indexing and Microsatellite Markers
Powdery mildew disease of oilseed mustard caused by Erysiphe cruciferarum is a primary reason of yield reduction not only in India but also throughout the world. Identification and cultivation of resistant mustard genotypes against powdery mildew is the only way to overcome this challenge. In the pr...
Saved in:
Published in | Current Journal of Applied Science and Technology Vol. 42; no. 16; pp. 54 - 66 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
28.06.2023
|
Online Access | Get full text |
Cover
Loading…
Abstract | Powdery mildew disease of oilseed mustard caused by Erysiphe cruciferarum is a primary reason of yield reduction not only in India but also throughout the world. Identification and cultivation of resistant mustard genotypes against powdery mildew is the only way to overcome this challenge. In the present investigation, we targeted to screen 75 Brassica genotypes against powdery mildew based on disease indexing under field conditions and gene-specific molecular markers. Disease reaction on both the cotyledonary and true leaves was screened with using a modified 0-9 scale score as well as with nineteen disease linked microsatellite markers. In disease indexing under field conditions, genotypes viz., L-4 and PC-5 were identified as immune, China and RP-9 were considered as highly resistant and GSC-7 and PC-6 were recognized as resistant whilst genotypes i.e., RB-50, Pusa Bold, WRR-10 and GSL-1 were accredited as moderately resistant. Molecular markers based UPGMA dendrogram classified Rohini, WRR-22, PC-6, PusaBold, China, WRR-8, GSL-1, WRR-7, RH-749, L-4 and RB-50 as highly resistant mustard genotypes. In addition, disease linked marker cnu_m616 had the highest polymorphic information content (0.75) with greatest ability to differentiate resistant genotypes from susceptible genotypes, may be employed directly in mustard breeding programmes in future. |
---|---|
AbstractList | Powdery mildew disease of oilseed mustard caused by Erysiphe cruciferarum is a primary reason of yield reduction not only in India but also throughout the world. Identification and cultivation of resistant mustard genotypes against powdery mildew is the only way to overcome this challenge. In the present investigation, we targeted to screen 75 Brassica genotypes against powdery mildew based on disease indexing under field conditions and gene-specific molecular markers. Disease reaction on both the cotyledonary and true leaves was screened with using a modified 0-9 scale score as well as with nineteen disease linked microsatellite markers. In disease indexing under field conditions, genotypes viz., L-4 and PC-5 were identified as immune, China and RP-9 were considered as highly resistant and GSC-7 and PC-6 were recognized as resistant whilst genotypes i.e., RB-50, Pusa Bold, WRR-10 and GSL-1 were accredited as moderately resistant. Molecular markers based UPGMA dendrogram classified Rohini, WRR-22, PC-6, PusaBold, China, WRR-8, GSL-1, WRR-7, RH-749, L-4 and RB-50 as highly resistant mustard genotypes. In addition, disease linked marker cnu_m616 had the highest polymorphic information content (0.75) with greatest ability to differentiate resistant genotypes from susceptible genotypes, may be employed directly in mustard breeding programmes in future. |
Author | Yadav, Rakesh Shrivastava, Aditi Tripathi, Niraj Parihar, Prerana Singh, J. Tripathi, Manoj Kumar Tiwari, Sushma Chauhan, Shailja Singh, Purnima Tiwari, Prakash N. |
Author_xml | – sequence: 1 givenname: Aditi surname: Shrivastava fullname: Shrivastava, Aditi – sequence: 2 givenname: Manoj Kumar surname: Tripathi fullname: Tripathi, Manoj Kumar – sequence: 3 givenname: Sushma surname: Tiwari fullname: Tiwari, Sushma – sequence: 4 givenname: Niraj surname: Tripathi fullname: Tripathi, Niraj – sequence: 5 givenname: Prakash N. surname: Tiwari fullname: Tiwari, Prakash N. – sequence: 6 givenname: Purnima surname: Singh fullname: Singh, Purnima – sequence: 7 givenname: Prerana surname: Parihar fullname: Parihar, Prerana – sequence: 8 givenname: Rakesh surname: Yadav fullname: Yadav, Rakesh – sequence: 9 givenname: Shailja surname: Chauhan fullname: Chauhan, Shailja – sequence: 10 givenname: J. surname: Singh fullname: Singh, J. |
BookMark | eNqdj81KAzEUhYNUsGofQbgvUCd_ddpt_V8URN2H6-SORKdJyQ3WeXtnRNC1q3MW5zvwHYtJTJGEOFPyfFUbWzVvyKXSUpvqw-qgLqwy8kBMtV3UcyW1nfzpR2LGHF6ktbUxq-VyKrZP1FFTQoqQWnhIe0-5h03oPO3hkThwwVhgnXEAG4Rbiqn0O2JYI5OHgbsKTEOH--jpM8RXwOiHhyYnxkJdFwrBBvM7ZT4Vhy12TLOfPBGLm-vny7v5OOZMrdvlsMXcOyXdqOe-9dyo5371zH-5LwnJYAc |
ContentType | Journal Article |
DBID | AAYXX CITATION |
DOI | 10.9734/cjast/2023/v42i164130 |
DatabaseName | CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | CrossRef |
DeliveryMethod | fulltext_linktorsrc |
EISSN | 2457-1024 |
EndPage | 66 |
ExternalDocumentID | 10_9734_cjast_2023_v42i164130 |
GroupedDBID | AAYXX CITATION M~E |
ID | FETCH-crossref_primary_10_9734_cjast_2023_v42i1641303 |
ISSN | 2457-1024 |
IngestDate | Fri Aug 23 03:40:10 EDT 2024 |
IsPeerReviewed | false |
IsScholarly | true |
Issue | 16 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-crossref_primary_10_9734_cjast_2023_v42i1641303 |
ParticipantIDs | crossref_primary_10_9734_cjast_2023_v42i164130 |
PublicationCentury | 2000 |
PublicationDate | 2023-06-28 |
PublicationDateYYYYMMDD | 2023-06-28 |
PublicationDate_xml | – month: 06 year: 2023 text: 2023-06-28 day: 28 |
PublicationDecade | 2020 |
PublicationTitle | Current Journal of Applied Science and Technology |
PublicationYear | 2023 |
SSID | ssib044733988 |
Score | 4.556649 |
Snippet | Powdery mildew disease of oilseed mustard caused by Erysiphe cruciferarum is a primary reason of yield reduction not only in India but also throughout the... |
SourceID | crossref |
SourceType | Aggregation Database |
StartPage | 54 |
Title | Selection of Powdery Mildew Resistant Brassica Genotypes Based on Disease Indexing and Microsatellite Markers |
Volume | 42 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT-MwELYKXLggECAeC_KBW5XSOk6zOfIUQipaqSBxi-LEEakgQWlaHgd-4_6knbEdN0C12u4lqlx5lPb7NDMez4OQI9blohv3uBO7UeBwwXwnAqwdnsrAC2QPu0NitsVN_-qOX997963W70bW0qQSnfh9bl3J_6AKa4ArVskugKwVCgvwGfCFJyAMz3_CeKiG2BiX71fxksjyrT3IHhOJQf8xuoY5nv_BQQYssMV0gSHXcfsUbFeC9wTn-n4G1EQiX-t6xQEm6Y0j1auzkqqcx-TJ254GpqvTHHe2VhYo6HvcfvhQZtMI3muq47lJVmU2eFBmaj6yLiHKi1Fb5X_br7OXSJfFDyfjh5k5aW67ycpo1AxkMBwqUReGK33HuOeDVdAl1R05Z80obM6axGyqX483DLme5vLVRAS-ywHXeBRhTc0l050rppxlcHDsmTuiT225v5hLm8QIxycUFipRIQoKZ2KWyArzAw-TTAcfF7WG49x33UANQ7W_TJeVoaRjJQnDUe7xTFLDYWp4PrfrZM1gTE80_zZIS-ab5MlyjxYpNdyjmnvUco_W3KOWe1Rxj8I-wz1ac48CZehn7lHDvS3iXV7cnl059TuGz7pLSvjXv8fdJst5kcsdQkXXS10wdV0RSx5xT_xM4YzAUiGSmPfj_i7pLCZ7b9EN-2R1xscfZLkqJ_IAXM1KHCr0_gA8DYfM |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | ISSN International Centre |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Selection+of+Powdery+Mildew+Resistant+Brassica+Genotypes+Based+on+Disease+Indexing+and+Microsatellite+Markers&rft.jtitle=Current+Journal+of+Applied+Science+and+Technology&rft.au=Shrivastava%2C+Aditi&rft.au=Tripathi%2C+Manoj+Kumar&rft.au=Tiwari%2C+Sushma&rft.au=Tripathi%2C+Niraj&rft.date=2023-06-28&rft.issn=2457-1024&rft.eissn=2457-1024&rft.volume=42&rft.issue=16&rft.spage=54&rft.epage=66&rft_id=info:doi/10.9734%2Fcjast%2F2023%2Fv42i164130&rft.externalDBID=n%2Fa&rft.externalDocID=10_9734_cjast_2023_v42i164130 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2457-1024&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2457-1024&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2457-1024&client=summon |