Viabilidade de Tritrichomonas foetus após congelamento com diferentes crioprotetores
Este trabalho teve como objetivo avaliar a viabilidade de um isolado de Tritrichomonas foetus criopreservada com glicerol e dimetilsulfóxido (DMSO) a -196ºC. Isolados do protozoário foram descongeladas dois dias após o congelamento e 6, 12, 18 e 26 meses, para avaliação de sua viabilidade. Em todos...
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| Published in | Revista brasileira de parasitologia veterinaria Vol. 17; no. 3; pp. 161 - 162 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
01.09.2008
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| Online Access | Get full text |
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| Abstract | Este trabalho teve como objetivo avaliar a viabilidade de um isolado de Tritrichomonas foetus criopreservada com glicerol e dimetilsulfóxido (DMSO) a -196ºC. Isolados do protozoário foram descongeladas dois dias após o congelamento e 6, 12, 18 e 26 meses, para avaliação de sua viabilidade. Em todos os tempos analisados, o congelamento foi viável em 60% e 90% das amostras congeladas com glicerol a 10% e DMSO a 12%, respectivamente.
The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196ºC. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60% and 90% of freeze samples with glycerol at 10% and DMSO at 12%, respectively. |
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| AbstractList | Este trabalho teve como objetivo avaliar a viabilidade de um isolado de Tritrichomonas foetus criopreservada com glicerol e dimetilsulfóxido (DMSO) a -196ºC. Isolados do protozoário foram descongeladas dois dias após o congelamento e 6, 12, 18 e 26 meses, para avaliação de sua viabilidade. Em todos os tempos analisados, o congelamento foi viável em 60% e 90% das amostras congeladas com glicerol a 10% e DMSO a 12%, respectivamente.
The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196ºC. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60% and 90% of freeze samples with glycerol at 10% and DMSO at 12%, respectively. |
| Author | Lobato, Francisco Carlos F. Salvarani, Felipe M. Martins, Nelson Éder Assis, Ronnie A. de Costa, Geraldo Márcio da Lima, Catarina G.R.D. Silva, Rodrigo Otávio S. Pires, Prhiscylla S. |
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| Cites_doi | 10.1111/j.1550-7408.1962.tb02632.x 10.1016/0035-9203(78)90144-X 10.2307/3274682 10.1128/CMR.17.4.794-803.2004 10.1111/j.1550-7408.1966.tb01893.x 10.1016/0304-4017(89)90083-6 10.1007/s00436-007-0559-y 10.1111/j.1550-7408.1969.tb02304.x |
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| References | LEVINE ND. (ref8) 1962; 9 MATSUO J. (ref9) 2007; 101 DIAMOND L.S. (ref3) 1957; 43 SCHWEBKE J.R. (ref11) 2004; 17 BONDURANT R.H. (ref1) 2005; 21 KULDA J. (ref6) 1969; 16 CAMPERO C.M. (ref2) 1989; 31 LEVINE N.D. (ref7) 1966; 13 DIAMOND L.S. (ref4) 1978; 72 RAE D.O. (ref10) 2006; 22 GUIDA H.G. (ref5) 1960 |
| References_xml | – volume: 9 start-page: 347 issn: 0022-3921 issue: 3 year: 1962 ident: ref8 article-title: Survival of Tritrichomonas foetus stored at -28 and -95 degrees C after freezing in the presence of glycerol publication-title: Journal of Protozoology doi: 10.1111/j.1550-7408.1962.tb02632.x contributor: fullname: LEVINE ND. – volume: 72 start-page: 431 issn: 0035-9203 issue: 4 year: 1978 ident: ref4 article-title: A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba publication-title: Transactions of the Royal Society of Tropical Medicine and Hygiene doi: 10.1016/0035-9203(78)90144-X contributor: fullname: DIAMOND L.S. – volume: 43 start-page: 488 issn: 0022-3395 issue: 4 year: 1957 ident: ref3 article-title: The establishment of various trichomonads of animals and man in axenic cultures publication-title: Journal of Parasitology doi: 10.2307/3274682 contributor: fullname: DIAMOND L.S. – volume: 22 start-page: 595 issue: 3 year: 2006 ident: ref10 article-title: Tritrichomonas foetus publication-title: Veterinary Clinics of North America: Food Animal Practice contributor: fullname: RAE D.O. – year: 1960 ident: ref5 contributor: fullname: GUIDA H.G. – volume: 17 start-page: 794 issn: 0893-8512 issue: 4 year: 2004 ident: ref11 article-title: Trichomoniasis publication-title: Clinical Microbiology Reviews doi: 10.1128/CMR.17.4.794-803.2004 contributor: fullname: SCHWEBKE J.R. – volume: 13 start-page: 199 issn: 0022-3921 issue: 2 year: 1966 ident: ref7 article-title: Frozen storage of Tritrichomonas foetus for 5.6 years publication-title: Journal of Protozoology doi: 10.1111/j.1550-7408.1966.tb01893.x contributor: fullname: LEVINE N.D. – volume: 31 start-page: 339 issn: 0304-4017 issue: 3-4 year: 1989 ident: ref2 article-title: Use of DMSO for the cryopreservation of Trichomonas foetus in liquid nitrogen publication-title: Veterinary Parasitology doi: 10.1016/0304-4017(89)90083-6 contributor: fullname: CAMPERO C.M. – volume: 101 start-page: 907 issn: 0932-0113 issue: 4 year: 2007 ident: ref9 article-title: A simple and rapid method for cryopreservation of Trichomonas vaginalis publication-title: Parasitology Research doi: 10.1007/s00436-007-0559-y contributor: fullname: MATSUO J. – volume: 21 start-page: 383 issue: 2 year: 2005 ident: ref1 article-title: Venereal diseases of cattle: natural history, diagnosis, and the role of vaccines in their control publication-title: Veterinary Clinics of North America: Food Animal Practice contributor: fullname: BONDURANT R.H. – volume: 16 start-page: 479 issn: 0022-3921 issue: 3 year: 1969 ident: ref6 article-title: Behavior and pathogenicity of Tritrichomonas foetus in chick liver cell cultures publication-title: Journal of Protozoology doi: 10.1111/j.1550-7408.1969.tb02304.x contributor: fullname: KULDA J. |
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