Atividade da catalase e da lactato desidrogenase em tilápias submetidas a estresse de confinamento: efeito da cor do ambiente

Avaliaram-se os efeitos da cor do ambiente sobre o crescimento e a atividade da enzima antioxidante catalase (CAT) e da lactato desidrogenase (LDH) em tilápias do Nilo (n=24; 36,2±3,6g). Oito exemplares foram mortos para determinação da atividade basal das enzimas e os demais permaneceram isolados d...

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Published inCiência rural Vol. 42; no. 5; pp. 894 - 899
Main Authors Pereira-da-Silva, Elyara Maria, Melo, Mariza Pires de, Oliveira, Ricardo Henrique Franco de, Pugine, Silvana Marina Piccoli
Format Journal Article
LanguageEnglish
Published 01.05.2012
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Abstract Avaliaram-se os efeitos da cor do ambiente sobre o crescimento e a atividade da enzima antioxidante catalase (CAT) e da lactato desidrogenase (LDH) em tilápias do Nilo (n=24; 36,2±3,6g). Oito exemplares foram mortos para determinação da atividade basal das enzimas e os demais permaneceram isolados durante 14 dias sob espectro de luz branca ou azul (n=8 peixes/tratamento). A seguir os peixes foram submetidos a um estresse diário de confinamento de 90 minutos (15° ao 28° dia) e pesados semanalmente para cálculo da taxa de crescimento específico (TCE). A TCE negativa confirmou que o confinamento provocou estresse nos peixes, independentemente da cor do ambiente. O aumento da atividade da LDH no músculo vermelho dos peixes mantidos sob luz branca ou azul indicou mudança do metabolismo aeróbio para anaeróbio. O estresse reduziu a atividade da CAT no músculo branco dos peixes mantidos sob a luz branca ou azul. Na musculatura vermelha, esta redução ocorreu apenas nos animais mantidos sob a luz branca. O confinamento aumenta os processos metabólicos anaeróbios e é adequado para estudos sobre os efeitos do estresse. O espectro de luz azul não evita a redução do crescimento e a demanda energética anaeróbia em situações de estresse, mas preserva a atividade da CAT, contribuindo para o bem-estar da tilápia. We assess the effects of the background color on the growth and antioxidant enzyme catalase (CAT) and lactate dehydrogenase (LDH) in Nile tilapia (n=24; 36.2±3.6g). Eight fish were killed for assessment of basal activity of the enzymes and the others remained isolated for 14 days under white or blue light spectrum (n=8 fish/treatment). Then each animal were subjected to a daily stress of confinement of 90 minutes (15th to 28th day) and weighed to calculate the specific growth rate (SGR). The negative SGR confirmed that the confinement stressed in fish, regardless of the background color. The increased activity of LDH in red muscle of fish kept under white or blue light indicated replacement of aerobic metabolism to anaerobic. Stress reduced the CAT activity in white muscle of fish kept under white or light blue. In red muscle this reduction occurred only in animals kept under white light. Restraint causes increase in anaerobic metabolic processes and is suitable for studies on the effects of stress in tilapia. Confinement increases the anaerobic metabolic processes and is suitable for studies on the effects of stress. The blue spectrum of light does not prevent the reduction of growth and the anaerobic energetic demand in stressful situations, but preserves the CAT activity, contributing to the tilápia welfare.
AbstractList Avaliaram-se os efeitos da cor do ambiente sobre o crescimento e a atividade da enzima antioxidante catalase (CAT) e da lactato desidrogenase (LDH) em tilápias do Nilo (n=24; 36,2±3,6g). Oito exemplares foram mortos para determinação da atividade basal das enzimas e os demais permaneceram isolados durante 14 dias sob espectro de luz branca ou azul (n=8 peixes/tratamento). A seguir os peixes foram submetidos a um estresse diário de confinamento de 90 minutos (15° ao 28° dia) e pesados semanalmente para cálculo da taxa de crescimento específico (TCE). A TCE negativa confirmou que o confinamento provocou estresse nos peixes, independentemente da cor do ambiente. O aumento da atividade da LDH no músculo vermelho dos peixes mantidos sob luz branca ou azul indicou mudança do metabolismo aeróbio para anaeróbio. O estresse reduziu a atividade da CAT no músculo branco dos peixes mantidos sob a luz branca ou azul. Na musculatura vermelha, esta redução ocorreu apenas nos animais mantidos sob a luz branca. O confinamento aumenta os processos metabólicos anaeróbios e é adequado para estudos sobre os efeitos do estresse. O espectro de luz azul não evita a redução do crescimento e a demanda energética anaeróbia em situações de estresse, mas preserva a atividade da CAT, contribuindo para o bem-estar da tilápia. We assess the effects of the background color on the growth and antioxidant enzyme catalase (CAT) and lactate dehydrogenase (LDH) in Nile tilapia (n=24; 36.2±3.6g). Eight fish were killed for assessment of basal activity of the enzymes and the others remained isolated for 14 days under white or blue light spectrum (n=8 fish/treatment). Then each animal were subjected to a daily stress of confinement of 90 minutes (15th to 28th day) and weighed to calculate the specific growth rate (SGR). The negative SGR confirmed that the confinement stressed in fish, regardless of the background color. The increased activity of LDH in red muscle of fish kept under white or blue light indicated replacement of aerobic metabolism to anaerobic. Stress reduced the CAT activity in white muscle of fish kept under white or light blue. In red muscle this reduction occurred only in animals kept under white light. Restraint causes increase in anaerobic metabolic processes and is suitable for studies on the effects of stress in tilapia. Confinement increases the anaerobic metabolic processes and is suitable for studies on the effects of stress. The blue spectrum of light does not prevent the reduction of growth and the anaerobic energetic demand in stressful situations, but preserves the CAT activity, contributing to the tilápia welfare.
Author Oliveira, Ricardo Henrique Franco de
Melo, Mariza Pires de
Pereira-da-Silva, Elyara Maria
Pugine, Silvana Marina Piccoli
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