Abstract WMP72: Peptide Composition of Stroke Causing Emboli Correlate With Serum Predictors of Atherosclerosis
Abstract only Introduction: The specific protein composition of stroke-causing emboli is unknown. Because ischemic stroke has a varied etiology, it is possible that the composition of the thrombus from which an embolus originated will have distinctive molecular characteristics reflective of the unde...
Saved in:
Published in | Stroke (1970) Vol. 48; no. suppl_1 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.02.2017
|
Online Access | Get full text |
Cover
Loading…
Abstract | Abstract only
Introduction:
The specific protein composition of stroke-causing emboli is unknown. Because ischemic stroke has a varied etiology, it is possible that the composition of the thrombus from which an embolus originated will have distinctive molecular characteristics reflective of the underlying pathophysiology. In this study, we used mass spectrometry to evaluate the protein composition of retrieved emboli from patients with differing stroke etiologies and correlated the protein levels to serum predictors of atherosclerosis.
Methods:
Emboli from 20 consecutive acute stroke patients were retrieved by embolectomy during routine stroke care. Thrombus proteins were extracted, digested and multidimentional fractionation of peptides was performed. Fractionated peptides underwent nano-liquid chromatography with tandem mass spectrometry. Spectra were searched using Mascot software in which results with p<0.05 (95% confidence interval) were considered significant and indicating identity. The results were correlated to A1C, LDL, and ESR taken on admission.
Results:
Demographics: 55% had atrial fibrillation, 20% had significant proximal vessel atherosclerosis, 10% were cryptogenic, and three had other identified etiologies (left ventricular thrombus, dissection, endocarditis). Eighty-one common proteins (eg hemoglobin, fibrin, actin) were found in all 20 emboli. Serum LDL levels correlated with Septin-2 (r
s
=0.78, p=0.028), Phosphoglycerate Kinase 1 (r
s
=0.75, p=0.036), Integrin Alpha-M (r
s
=0.68, p=0.033) and Glucose-6-phosphate dehydrogenase (r
s
=0.63, p=0.05). Septin-7 levels inversely correlated to ESR (r
s
= -0.84, p=0.01). No significant protein correlations to A1C were found.
Conclusion:
To our knowledge, this study is the first mass spectrometry analysis of thrombi retrieved from acute stroke patients and the first to correlate the thrombus proteome to clinical features of the patient. Notably, we found proteins associated with inflammation (eg Integrin Alpha–M) in emboli from patients with high LDL. Although these findings are tempered by a small sample size, we provide preliminary support for the feasibility of utilizing proteomic analysis of emboli to discover proteins that may be used as markers for stroke etiology. |
---|---|
AbstractList | Abstract only
Introduction:
The specific protein composition of stroke-causing emboli is unknown. Because ischemic stroke has a varied etiology, it is possible that the composition of the thrombus from which an embolus originated will have distinctive molecular characteristics reflective of the underlying pathophysiology. In this study, we used mass spectrometry to evaluate the protein composition of retrieved emboli from patients with differing stroke etiologies and correlated the protein levels to serum predictors of atherosclerosis.
Methods:
Emboli from 20 consecutive acute stroke patients were retrieved by embolectomy during routine stroke care. Thrombus proteins were extracted, digested and multidimentional fractionation of peptides was performed. Fractionated peptides underwent nano-liquid chromatography with tandem mass spectrometry. Spectra were searched using Mascot software in which results with p<0.05 (95% confidence interval) were considered significant and indicating identity. The results were correlated to A1C, LDL, and ESR taken on admission.
Results:
Demographics: 55% had atrial fibrillation, 20% had significant proximal vessel atherosclerosis, 10% were cryptogenic, and three had other identified etiologies (left ventricular thrombus, dissection, endocarditis). Eighty-one common proteins (eg hemoglobin, fibrin, actin) were found in all 20 emboli. Serum LDL levels correlated with Septin-2 (r
s
=0.78, p=0.028), Phosphoglycerate Kinase 1 (r
s
=0.75, p=0.036), Integrin Alpha-M (r
s
=0.68, p=0.033) and Glucose-6-phosphate dehydrogenase (r
s
=0.63, p=0.05). Septin-7 levels inversely correlated to ESR (r
s
= -0.84, p=0.01). No significant protein correlations to A1C were found.
Conclusion:
To our knowledge, this study is the first mass spectrometry analysis of thrombi retrieved from acute stroke patients and the first to correlate the thrombus proteome to clinical features of the patient. Notably, we found proteins associated with inflammation (eg Integrin Alpha–M) in emboli from patients with high LDL. Although these findings are tempered by a small sample size, we provide preliminary support for the feasibility of utilizing proteomic analysis of emboli to discover proteins that may be used as markers for stroke etiology. |
Author | Rao, Neal M Whitelegge, Julian P Liebeskind, David S Abdaljaleel, Maram Y Restrepo-Jimenez, Lucas Yong, William H Cohn, Whitaker Capri, Joseph Gornbein, Jeffrey A |
Author_xml | – sequence: 1 givenname: Neal M surname: Rao fullname: Rao, Neal M organization: Neurology, David Geffen Sch Of Medicine at UCLA, Los Angeles, CA – sequence: 2 givenname: Joseph surname: Capri fullname: Capri, Joseph organization: Univ of California, Los Angeles, Los Angeles, CA – sequence: 3 givenname: Whitaker surname: Cohn fullname: Cohn, Whitaker organization: Univ of California, Los Angeles, Los Angeles, CA – sequence: 4 givenname: Maram Y surname: Abdaljaleel fullname: Abdaljaleel, Maram Y organization: Pathology, David Geffen Sch Of Medicine at UCLA, Los Angeles, CA – sequence: 5 givenname: Lucas surname: Restrepo-Jimenez fullname: Restrepo-Jimenez, Lucas organization: Neurology, David Geffen Sch Of Medicine at UCLA, Los Angeles, CA – sequence: 6 givenname: Jeffrey A surname: Gornbein fullname: Gornbein, Jeffrey A organization: Biomathematics, David Geffen Sch Of Medicine at UCLA, Los Angeles, CA – sequence: 7 givenname: William H surname: Yong fullname: Yong, William H organization: Dept of Pathology and Laboratory Medicine, David Geffen Sch Of Medicine at UCLA, Los Angeles, CA – sequence: 8 givenname: David S surname: Liebeskind fullname: Liebeskind, David S organization: Neurology, David Geffen Sch Of Medicine at UCLA, Los Angeles, CA – sequence: 9 givenname: Julian P surname: Whitelegge fullname: Whitelegge, Julian P organization: NPI-Semel Institute, David Geffen Sch of Medicine at UCLA, Los Angeles, CA |
BookMark | eNqdj0FqwzAQRUVJoE6bG3ShC9iVZCWxuwshpZuCIYUshePIjVrJEjMypbevXHKCbObDzDz4b0Fmgx80IU-cFZyv-TNGKGRV4BiCVbz4cWEj7kjGV0Lmci2qGckYK-tcyLq-JwvEL8aYKKtVRvz2lOi2i_T43mzEC210iOas6c674NFE4wfqe3qI4L_Tth3RDJ90707emvQEoG0bNT2aeKEHDaOjDeiz6aIHnMBtvGjw2NlpGnwk8761qJfXfCDydf-xe8u7dEbQvQpgXAu_ijM1yalUT8lKXeXUv1x5I_YHKLVjBw |
ContentType | Journal Article |
DBID | AAYXX CITATION |
DOI | 10.1161/str.48.suppl_1.wmp72 |
DatabaseName | CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | CrossRef |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1524-4628 |
ExternalDocumentID | 10_1161_str_48_suppl_1_wmp72 |
GroupedDBID | --- .3C .55 .GJ .XZ .Z2 01R 0R~ 123 1J1 2WC 3O- 40H 4Q1 4Q2 4Q3 53G 5RE 5VS 6PF 71W 77Y 7O~ A9M AAAAV AAAXR AAGIX AAHPQ AAIQE AAJCS AAMOA AAMTA AAQKA AAQQT AARTV AASCR AASOK AAUEB AAXQO AAYEP AAYJJ AAYXX ABASU ABBUW ABDIG ABJNI ABQRW ABVCZ ABXVJ ABZAD ACCJW ACDDN ACEWG ACGFS ACGOD ACILI ACLDA ACWDW ACWRI ACXJB ACXNZ ADBBV ADFPA ADGGA ADHPY ADNKB AE3 AE6 AEBDS AEETU AENEX AFDTB AFEXH AFFNX AFSOK AFUWQ AGINI AHMBA AHOMT AHQNM AHRYX AHVBC AIJEX AINUH AJIOK AJNWD AJNYG AJZMW AKULP ALMA_UNASSIGNED_HOLDINGS ALMTX AMJPA AMKUR AMNEI AOHHW AWKKM AYCSE BAWUL BCGUY BOYCO BQLVK BS7 C45 CITATION CS3 DIK DIWNM DU5 DUNZO E.X E3Z EBS EEVPB EJD ERAAH EX3 F2K F2L F2M F2N F5P FCALG FL- FW0 GNXGY GQDEL GX1 H0~ H13 HLJTE HZ~ IKREB IKYAY IN~ IPNFZ J5H JF9 JG8 JK3 JK8 K8S KD2 KMI KQ8 L-C L7B M18 N4W N9A N~7 N~B N~M O9- OAG OAH OB3 OCUKA ODA ODMTH OGROG OHYEH OJAPA OK1 OL1 OLG OLH OLU OLV OLW OLY OLZ OPUJH ORVUJ OUVQU OVD OVDNE OVIDH OVLEI OVOZU OWBYB OWU OWV OWW OWX OWY OWZ OXXIT P-K P2P PQQKQ R58 RAH RHF RIG RLZ S4R S4S T8P TEORI TSPGW V2I VVN W3M W8F WH7 WOQ WOW X3V X3W X7M XXN XYM YCJ YFH YHZ YQJ YYP ZB8 ZGI ZZMQN |
ID | FETCH-crossref_primary_10_1161_str_48_suppl_1_wmp723 |
ISSN | 0039-2499 |
IngestDate | Fri Dec 06 01:40:16 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | suppl_1 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-crossref_primary_10_1161_str_48_suppl_1_wmp723 |
ParticipantIDs | crossref_primary_10_1161_str_48_suppl_1_wmp72 |
PublicationCentury | 2000 |
PublicationDate | 2017-02-00 |
PublicationDateYYYYMMDD | 2017-02-01 |
PublicationDate_xml | – month: 02 year: 2017 text: 2017-02-00 |
PublicationDecade | 2010 |
PublicationTitle | Stroke (1970) |
PublicationYear | 2017 |
SSID | ssj0002385 |
Score | 4.5211353 |
Snippet | Abstract only
Introduction:
The specific protein composition of stroke-causing emboli is unknown. Because ischemic stroke has a varied etiology, it is possible... |
SourceID | crossref |
SourceType | Aggregation Database |
Title | Abstract WMP72: Peptide Composition of Stroke Causing Emboli Correlate With Serum Predictors of Atherosclerosis |
Volume | 48 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8NAEF5qBfEiPvHNHrxJYtO8Wm9FKkVJqVKxt7DbpFT7SElTBP-Ef9mZ3W26liLWyxKW7STNfJmdmZ0HIVc-FnFjXdvo9lwwUCo9y2CRYxue78cc1AfPEp3ngqbXeHEeOm6nUPjSopZmGTe7nyvzSv7DVZgDvmKW7BqczYnCBFwDf2EEDsP4Jx7XODoqutn1a9Dyy2jctzBIJYrFZ67CsURaSpYmA5hlM-EaqI94MnyDRalIZYlBNmR9FBsYFZ_i0Y3owYNKKuqHyRTuC-PbVFdlFU0s9FT1S5pH4ZkJ92sTSxZrZxwTmdOujhzm00lfiD3s08cGi0jhGo_Y8B02LxlEELCUjdRWoVwUsO2V8nAPJXZtPMaRnZDMWEnasmNgYqwuip2KBrkptjUNrdVy3kM5D-_YdCqmWmh-jCayDdDPstpL210ehCjMH88KgUroVEJFJRRUNsgmVlbEZgyPT4vy86DfyJYY6v-oXEygcrPqWTRdR1Na2rtkR1kbtCahs0cK8XifbAUqnuKAJHMEUYGgW6rwQzX80KRHJa-pwg-V-KE5fijihwr80AV-8IdL-Dkkzn29fdcw5g8cTmS1k_C312QfkeI4GcfHhHpu2YNN3LUi33Z6rss4fPgRWB_ccrlbrp6Q9Uifrrn-jGwvoHdOilk6iy9AX8z4peDhN1H6dxw |
link.rule.ids | 314,780,784,27924,27925 |
linkProvider | Colorado Alliance of Research Libraries |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Abstract+WMP72%3A+Peptide+Composition+of+Stroke+Causing+Emboli+Correlate+With+Serum+Predictors+of+Atherosclerosis&rft.jtitle=Stroke+%281970%29&rft.au=Rao%2C+Neal+M&rft.au=Capri%2C+Joseph&rft.au=Cohn%2C+Whitaker&rft.au=Abdaljaleel%2C+Maram+Y&rft.date=2017-02-01&rft.issn=0039-2499&rft.eissn=1524-4628&rft.volume=48&rft.issue=suppl_1&rft_id=info:doi/10.1161%2Fstr.48.suppl_1.wmp72&rft.externalDBID=n%2Fa&rft.externalDocID=10_1161_str_48_suppl_1_wmp72 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0039-2499&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0039-2499&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0039-2499&client=summon |