氯化钙浓度对念珠菌溶血活性的影响

目的通过将固定浓度的念珠菌菌悬液分别滴加到含有不同浓度CaCl2的羊血培养基上培养，观察念珠菌溶血活性的差异，评估CaCl2对念珠菌溶血活性的影响。方法选择25株念珠菌，其中包括21株临床分离株和4株标准株，配制4个不同浓度CaCl2的羊血培养基，分别为0%、0.5%、1%和2.5%（g/mL），于37℃、5%体积分数CO2培养。观察24h、48h、72h的溶血反应并拍照、记录。结果念珠菌在不同浓度CaCl2的羊血培养基中的溶血指数存在差异，差异具有统计学意义（F=24.929，P<0.001）。其中不含CaCl2的溶血指数为2.749±0.103，含0.5%、1%和2.5%依次...

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Published in口腔疾病防治 Vol. 25; no. 8; pp. 494 - 497
Main Author 万蕾;卢海宾;罗刚
Format Journal Article
LanguageChinese
Published 2017
Subjects
念珠菌;毒力因素;溶血活性;电解质;氯化钙
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Abstract 目的通过将固定浓度的念珠菌菌悬液分别滴加到含有不同浓度CaCl2的羊血培养基上培养，观察念珠菌溶血活性的差异，评估CaCl2对念珠菌溶血活性的影响。方法选择25株念珠菌，其中包括21株临床分离株和4株标准株，配制4个不同浓度CaCl2的羊血培养基，分别为0%、0.5%、1%和2.5%（g/mL），于37℃、5%体积分数CO2培养。观察24h、48h、72h的溶血反应并拍照、记录。结果念珠菌在不同浓度CaCl2的羊血培养基中的溶血指数存在差异，差异具有统计学意义（F=24.929，P<0.001）。其中不含CaCl2的溶血指数为2.749±0.103，含0.5%、1%和2.5%依次为2.247±0.079、2.013±0.092和2.150±0.066。结论不同浓度的CaCl2会影响羊血培养基上念珠菌的溶血活性。
AbstractList 目的通过将固定浓度的念珠菌菌悬液分别滴加到含有不同浓度CaCl2的羊血培养基上培养，观察念珠菌溶血活性的差异，评估CaCl2对念珠菌溶血活性的影响。方法选择25株念珠菌，其中包括21株临床分离株和4株标准株，配制4个不同浓度CaCl2的羊血培养基，分别为0%、0.5%、1%和2.5%（g/mL），于37℃、5%体积分数CO2培养。观察24h、48h、72h的溶血反应并拍照、记录。结果念珠菌在不同浓度CaCl2的羊血培养基中的溶血指数存在差异，差异具有统计学意义（F=24.929，P<0.001）。其中不含CaCl2的溶血指数为2.749±0.103，含0.5%、1%和2.5%依次为2.247±0.079、2.013±0.092和2.150±0.066。结论不同浓度的CaCl2会影响羊血培养基上念珠菌的溶血活性。
Author 万蕾;卢海宾;罗刚
AuthorAffiliation 南方医科大学口腔医院牙周种植科,广东广州510280;广州医科大学附属口腔医院·广州口腔疾病研究所·口腔医学重点实验室,广东广州510280
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DocumentTitleAlternate Influence of concentrations of CaCl2on hemolytic activity of candida strains
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Objective To investigate the influence of different concentrations of CaCl2on the hemolytic activity of candida strains.Methods A total of25candida strains were selected,including21candida strains recovered from clinical specimens,and4reference strains of C.albicans(ATCC90028)(American Type Culture Collection,Manassas,VA,USA),C.glabrata(ATCC90030),C.krusei(ATCC6258),and C.tropicalis(ATCC13803)respectively.CaCl2was added at concentrations of0%,0.5%,1%and2.5g%(wt/vol).Plates were cultured at37℃in a5%CO2atmosphere for24?72h,and the hemolytic index(Hi)was analyzed.Results Comparsions of the hemolytic indices among the groups treated with0.5%CaCl2(Hi=2.247±0.079),1%CaCl2(Hi=2.013±0.092),2.5%CaCl2(Hi=2.150±0.066)and the control(Hi=2.749±0.103)reached statistical significance(P<0.001).Conclusion CaCl2may produce a decrease activity in the hemolysis of candida species.
Candida species; Virulence factors; Hemolytic activity; Electrolytes; CaCl2
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SubjectTerms 念珠菌;毒力因素;溶血活性;电解质;氯化钙
Title 氯化钙浓度对念珠菌溶血活性的影响
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