Cloning of Small Heat-shock Protein （sHSP） Gene from Sugarcaneand Analysis of Its Expression under Drought Stress

The present experiment was conducted to clone small heat-shock protein （sHSP） in sugarcane to explore mechanism of stress resistance in sugarcane. sHSP was amplified using RT-PCR from sugarcane leaves, the characteristics of the deduced protein wereanalyzed using bioinformatics software, and its exp...

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Bibliographic Details
Published in农业生物技术：英文版 Vol. 6; no. 2; pp. 6 - 10
Main Author Panxia LIANG Jiangtao ZHOU Xing HUANG Yangrui LI
Format Journal Article
LanguageEnglish
Published 2017
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Summary:The present experiment was conducted to clone small heat-shock protein （sHSP） in sugarcane to explore mechanism of stress resistance in sugarcane. sHSP was amplified using RT-PCR from sugarcane leaves, the characteristics of the deduced protein wereanalyzed using bioinformatics software, and its expression was analyzed using quantitative real-time PCR. The results showed that the eDNA of HSP gene obtained by RT-PCR which was 659 bp in full length with a 459 bp open reading frame, encodes a putative sHSP protein with 152 amino acids. Homology comparison between the amino acid sequences of the sHSP protein and 14 di[- ferent species indicated that the sHSP protein had 69% to 96% identity in amino acid sequence with other plants. The deduced amino acid sequence not only conrained a typical sHSP domain, but also was very conservative. The results of quantitative real-time PCR analysis showed that the mRNA of sHSP decreased inilially and then increased with time under drought stress. These results suggested that sHSP is expressed under induction of drought stress, anddrought resistance in sugarcane is enhanced by Si application.
Bibliography:Sugarcane ; Drought ; Silicon ; HSP
The present experiment was conducted to clone small heat-shock protein （sHSP） in sugarcane to explore mechanism of stress resistance in sugarcane. sHSP was amplified using RT-PCR from sugarcane leaves, the characteristics of the deduced protein wereanalyzed using bioinformatics software, and its expression was analyzed using quantitative real-time PCR. The results showed that the eDNA of HSP gene obtained by RT-PCR which was 659 bp in full length with a 459 bp open reading frame, encodes a putative sHSP protein with 152 amino acids. Homology comparison between the amino acid sequences of the sHSP protein and 14 di[- ferent species indicated that the sHSP protein had 69% to 96% identity in amino acid sequence with other plants. The deduced amino acid sequence not only conrained a typical sHSP domain, but also was very conservative. The results of quantitative real-time PCR analysis showed that the mRNA of sHSP decreased inilially and then increased with time under drought stress. These results suggested that sHSP is expressed under induction of drought stress, anddrought resistance in sugarcane is enhanced by Si application.
ISSN:2164-4993