Comparative proteomic analysis provides new insights into ear leaf senescence of summer maize（Zea mays L.） under fi eld condition

As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular mechanisms of leaf senescence, protein expression in senescing maize ear leaves gro...

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Published in农业科学学报：英文版 no. 5; pp. 1005 - 1016
Main Author WEI Shan-shan WANG Xiang-yu LIU Peng ZHANG Ji-wang ZHAO Bin DONG Shu-ting
Format Journal Article
LanguageEnglish
Published 2016
Subjects
RNA结合蛋白
双向凝胶电泳
叶片衰老
基质辅助激光解吸
夏玉米
学分
比较蛋白质组
穗位叶
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Summary:As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular mechanisms of leaf senescence, protein expression in senescing maize ear leaves grown under field conditions was analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry（MALDI-TOF/TOF MS）. A total of 60 senescence-associated proteins were identified. The identified proteins are involved in many biological processes, especially energy, metabolism and protein synthesis. Several of the identified proteins have not been previously reported as senescence-associated, including glycine-rich RNA-binding protein.
Bibliography:WEI Shan-shan, WANG Xiang-yu, LIU Peng, ZHANG Ji-wang, ZHAO Bin, DONG Shu-ting（ State Key Laboratory of Crop Biology/National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources/ College of Agronomy, Shandong Agricultural University, Tai＇an 271018, P.R. China）
As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular mechanisms of leaf senescence, protein expression in senescing maize ear leaves grown under field conditions was analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry（MALDI-TOF/TOF MS）. A total of 60 senescence-associated proteins were identified. The identified proteins are involved in many biological processes, especially energy, metabolism and protein synthesis. Several of the identified proteins have not been previously reported as senescence-associated, including glycine-rich RNA-binding protein.
10-1039/S
maize （Zea mays L.）, ear leaf, proteomics, senescence
ISSN:2095-3119
2352-3425