小麦耐热相关转录因子基因TabZIP28的分离及功能分析

碱性亮氨酸拉链（basic leucine zipper,b ZIP）是植物中广泛存在的一类转录因子,参与多种胁迫响应与生长发育过程。本研究从小麦（Triticum aestivum）中克隆到一个热胁迫诱导的b ZIP家族转录因子基因Tab ZIP28（Gen Bank登录号：KT753298.1）,ORF长度为1 713 bp,编码570个氨基酸。生物信息学分析结果表明,Tab ZIP28与拟南芥b ZIP家族转录因子中B亚组的3个基因Atb ZIP17、Atb ZIP28和Atb ZIP49归为一类。氨基酸序列比对结果表明,该蛋白具有b ZIP和跨膜结构域（transmembrane do...

Full description

Saved in:
Bibliographic Details
Published in农业生物技术学报 Vol. 24; no. 2; pp. 157 - 167
Main Author 耿晓丽 臧新山 王飞 张丽媛 田雪军 倪中福 姚颖垠 胡兆荣 辛明明 彭惠茹 孙其信
Format Journal Article
LanguageChinese
Published 2016
Subjects
b
ZIP28
小麦
拟南芥
耐热性
转基因
Online AccessGet full text

Cover

More Information
Summary:碱性亮氨酸拉链（basic leucine zipper,b ZIP）是植物中广泛存在的一类转录因子,参与多种胁迫响应与生长发育过程。本研究从小麦（Triticum aestivum）中克隆到一个热胁迫诱导的b ZIP家族转录因子基因Tab ZIP28（Gen Bank登录号：KT753298.1）,ORF长度为1 713 bp,编码570个氨基酸。生物信息学分析结果表明,Tab ZIP28与拟南芥b ZIP家族转录因子中B亚组的3个基因Atb ZIP17、Atb ZIP28和Atb ZIP49归为一类。氨基酸序列比对结果表明,该蛋白具有b ZIP和跨膜结构域（transmembrane domain,TMD）两个保守结构域以及规范的位点1蛋白酶（site 1 protease,S1P）剪切位点。对该基因起始密码子ATG上游1 699bp的序列进行顺式作用元件分析,发现该基因的启动子区域包含众多激素和逆境胁迫响应元件。通过q RT-PCR对该基因在逆境胁迫下的表达模式进行分析,结果表明,Tab ZIP28在热胁迫处理1 h即上调表达且达到最大值;用20%PEG 6000模拟干旱环境处理小麦幼苗后,Tab ZIP28在处理6 h达到最大值,并在12 h时急剧下降;对5 mmol/L H2O2处理响应比较缓慢,在处理12 h才上调表达;该基因不受到二硫苏糖醇（dithiothreitol,DTT）处理的诱导表达。在拟南芥（Arabidopsis thaliana）中过量表达Tab ZIP28基因,转基因株系在高温胁迫后的成活率和种子发芽率较野生型明显提高,说明该基因可能对植物的耐热性有贡献,可以作为耐热性育种的候选基因。
Bibliography:Basic leucine zipper（bZIP） transcription factor family widely distributes in plant and involves in multiple stress response and developmental stages. In this study,we cloned a heat induced transcription factor gene Tab ZIP28（Gen Bank accession No. KT753298.1） from wheat（Triticum aestivum）,the length of ORF was 1 713 bp,encoding a protein of 570 amino acid residues. Sequence analysis indicated that Tab ZIP28 possessed a conserved b ZIP domain,a transmembrane domain（TMD） and a canonical site 1 protease（S1P）cleavage site. The promoter region of Tab ZIP28 contained several stress related cis- element. The expression patterns of Tab ZIP28 were analyzed by q RT-PCR under stress conditions. The results showed that Tab ZIP28 was induced by heat and peaked at 1 h; the transcript level of Tab ZIP28 was increased to the highest level by 20% PEG treatment for 6 h and reduced rapidly after 12 h; Tab ZIP28 was found to be slightly up-regulated by treatment of 5 mmol/L H2O2 for 12 h and Tab ZIP28 could not be induced by DTT
ISSN:1674-7968