肠道损伤对草鱼胆固醇代谢通路基因表达的影响
[目的]有研究显示草鱼肠道健康与胆固醇代谢具有较为紧密的关系,本研究探讨草鱼肠道损伤对胆固醇合成代谢通路基因表达的影响。[方法]以池塘养殖条件下的草鱼为研究对象,在对肠道损伤进行外观形态、组织切片和血清指标评估的基础上,测定了草鱼血清中的甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、总胆汁酸（TBA）和总胆固醇（TC）含量,采用荧光定量PCR（qRT-PCR）方法,定量检测肠道和肝胰脏组织的胆固醇、胆汁酸生物合成酶和调节控制酶的基因表达水平的变化。[结果]肠道损伤的草鱼血清HDL、LDL、TC和TBA的含量显著下降（P〈0.05）,肝胰脏和肠道组织的胆固醇、胆汁酸合成酶相关...
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| Published in | 南京农业大学学报 Vol. 38; no. 3; pp. 497 - 503 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
2015
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| Subjects | |
| Online Access | Get full text |
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| Abstract | [目的]有研究显示草鱼肠道健康与胆固醇代谢具有较为紧密的关系,本研究探讨草鱼肠道损伤对胆固醇合成代谢通路基因表达的影响。[方法]以池塘养殖条件下的草鱼为研究对象,在对肠道损伤进行外观形态、组织切片和血清指标评估的基础上,测定了草鱼血清中的甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、总胆汁酸（TBA）和总胆固醇（TC）含量,采用荧光定量PCR（qRT-PCR）方法,定量检测肠道和肝胰脏组织的胆固醇、胆汁酸生物合成酶和调节控制酶的基因表达水平的变化。[结果]肠道损伤的草鱼血清HDL、LDL、TC和TBA的含量显著下降（P〈0.05）,肝胰脏和肠道组织的胆固醇、胆汁酸合成酶相关基因表达水平极显著上调（P〈0.01）,与此同时,负调控胆汁酸合成的法尼醇X受体FXR和调控胆固醇逆转运途经的血浆胆固醇酯转移蛋白CETP基因表达水平极显著下调（P〈0.01）。[结论]草鱼肠道损伤后,胆固醇、胆汁酸的重吸收可能遭到破坏,刺激了肝胰脏和肠道的胆固醇、胆汁酸生物合成强度,从而维持胆固醇在机体内的代谢平衡。 |
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| AbstractList | [目的]有研究显示草鱼肠道健康与胆固醇代谢具有较为紧密的关系,本研究探讨草鱼肠道损伤对胆固醇合成代谢通路基因表达的影响。[方法]以池塘养殖条件下的草鱼为研究对象,在对肠道损伤进行外观形态、组织切片和血清指标评估的基础上,测定了草鱼血清中的甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、总胆汁酸（TBA）和总胆固醇（TC）含量,采用荧光定量PCR（qRT-PCR）方法,定量检测肠道和肝胰脏组织的胆固醇、胆汁酸生物合成酶和调节控制酶的基因表达水平的变化。[结果]肠道损伤的草鱼血清HDL、LDL、TC和TBA的含量显著下降（P〈0.05）,肝胰脏和肠道组织的胆固醇、胆汁酸合成酶相关基因表达水平极显著上调（P〈0.01）,与此同时,负调控胆汁酸合成的法尼醇X受体FXR和调控胆固醇逆转运途经的血浆胆固醇酯转移蛋白CETP基因表达水平极显著下调（P〈0.01）。[结论]草鱼肠道损伤后,胆固醇、胆汁酸的重吸收可能遭到破坏,刺激了肝胰脏和肠道的胆固醇、胆汁酸生物合成强度,从而维持胆固醇在机体内的代谢平衡。 |
| Author | 黄雨薇 叶元土 蔡春芳 刘汉超 陈科全 张宝彤 萧培珍 彭侃 徐登辉 |
| AuthorAffiliation | 苏州大学基础医学与生物科学学院,江苏苏州215123 北京营养源研究所／水产动物系统营养研究开放实验室,北京100000 |
| Author_xml | – sequence: 1 fullname: 黄雨薇 叶元土 蔡春芳 刘汉超 陈科全 张宝彤 萧培珍 彭侃 徐登辉 |
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| DocumentTitleAlternate | The effect of the gene expression in metabolism of cholesterol synthesis pathway after intestine injuried on Ctenopharyngodon idellus |
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| Notes | HUANG Yuwei, YE Yuantu, CAI Chunfang, LIU Hanchao , CHEN Kequan, ZHANG Baotong, XIAO Peizhen, PENG Kan, XU Denghui（ 1. School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China;2. Beijing Institute of Nutrition Source/Aquatic Animal Nutrition Research Laboratory of Systems,Beijing 100000,China） Ctenopharyngodon ideUus ; intestine injuried; bile acid ; enterohepatic axis ; gene expression [ Objectives] It has also been demonstrated that healthy intestine and cholesterol metabolism have a relatively close relationship. [ Methods ] In order to investigate the effect of the gene expression in cholesterol metabolism pathway after intestine injuried in grass carp, healthy intestine and damaged intestine in grass carp under culture conditions were selected on the basis of intes- tinal damage morphology, tissue biopsy and serum markers assessed, grass carp serum triglyceride （ TG）, high density lipoprotein （HDL） ,low density lipoprotein （ LDL）, total bile acid （TBA） and total cholesterol （ |
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| PublicationTitle | 南京农业大学学报 |
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| PublicationYear | 2015 |
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| StartPage | 497 |
| SubjectTerms | 中肠损伤 基因表达 胆固醇 胆汁酸 草鱼 |
| Title | 肠道损伤对草鱼胆固醇代谢通路基因表达的影响 |
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