High specificity detection of Pb^2＋ ions by p-SCN-Bz-DTPA immunogen and p-NH2-Bn-DTPA coating antigen

• Published in: 中国环境科学与工程前沿：英文版 no. 5; pp. 729 - 736
• Main Author: Ruozhen YU Zheng FANG Wei MENG Zhenguang YAN Lina DU Hong WANG Zhengtao LIU
• Format: Journal Article
• Language: English
• Published: 2014
• Subjects: 免疫原; 包被抗原; 特异性检测; 酶联免疫吸附测定; 重金属离子; 金属螯合物; 铅离子; 间接竞争ELISA

Only one bifunctional metal-chelator was used to prepare immunogen and coating antigen in all of the previous researches. However, the antibody-specific recognition to the spacer arm of the bifunctional metal- chelator might lower the specificity of heavy metal ions immunoassay. Two different bifunc...