Quantitative study of protein coronas on gold nano- particles with different surface modifications
Protein coronas provide the biological identity of nanomaterials in vivo. Here we have used dynamic light scattering （DLS） and transmission electron microscopy （TEM） to investigate the adsorption of serum proteins, including bovine serum albumin （BSA）, transferrin （TRF） and fibrinogen （FIB）, on gold...
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| Published in | 纳米研究：英文版 no. 3; pp. 345 - 352 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
2014
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Protein coronas provide the biological identity of nanomaterials in vivo. Here we have used dynamic light scattering （DLS） and transmission electron microscopy （TEM） to investigate the adsorption of serum proteins, including bovine serum albumin （BSA）, transferrin （TRF） and fibrinogen （FIB）, on gold nanoparticles （AuNPs） with different surface modifications （citrate, thioglycolic acid, cysteine, polyethylene glycol （PEG, Mw = 2 k and 5 k））. AuNPs with PEG（5 k） surface modification showed no protein adsorption. AuNPs with non-PEG surface modifications showed aggregation with FIB. AuNPs with citrate and thioglycolic acid surface modifications showed 6-8 nm thick BSA and TRF coronas （corres- ponding to monolayer or bilayer proteins）, in which the microscopic dissociation constants of BSA and TRF protein coronas are in the range of 104 to 104 M. |
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| AbstractList | Protein coronas provide the biological identity of nanomaterials in vivo. Here we have used dynamic light scattering （DLS） and transmission electron microscopy （TEM） to investigate the adsorption of serum proteins, including bovine serum albumin （BSA）, transferrin （TRF） and fibrinogen （FIB）, on gold nanoparticles （AuNPs） with different surface modifications （citrate, thioglycolic acid, cysteine, polyethylene glycol （PEG, Mw = 2 k and 5 k））. AuNPs with PEG（5 k） surface modification showed no protein adsorption. AuNPs with non-PEG surface modifications showed aggregation with FIB. AuNPs with citrate and thioglycolic acid surface modifications showed 6-8 nm thick BSA and TRF coronas （corres- ponding to monolayer or bilayer proteins）, in which the microscopic dissociation constants of BSA and TRF protein coronas are in the range of 104 to 104 M. |
| Author | Menghua Cui Renxiao Liu Zhaoyi Deng Guanglu Ge Ying Liu Liming Xie |
| AuthorAffiliation | CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology,Beijing 100190, China |
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| Notes | Protein coronas provide the biological identity of nanomaterials in vivo. Here we have used dynamic light scattering （DLS） and transmission electron microscopy （TEM） to investigate the adsorption of serum proteins, including bovine serum albumin （BSA）, transferrin （TRF） and fibrinogen （FIB）, on gold nanoparticles （AuNPs） with different surface modifications （citrate, thioglycolic acid, cysteine, polyethylene glycol （PEG, Mw = 2 k and 5 k））. AuNPs with PEG（5 k） surface modification showed no protein adsorption. AuNPs with non-PEG surface modifications showed aggregation with FIB. AuNPs with citrate and thioglycolic acid surface modifications showed 6-8 nm thick BSA and TRF coronas （corres- ponding to monolayer or bilayer proteins）, in which the microscopic dissociation constants of BSA and TRF protein coronas are in the range of 104 to 104 M. 11-5974/O4 protein corona,gold nanoparticle,dynamic light scattering,transmission electronmicroscopy,surface modification |
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| Snippet | Protein coronas provide the biological identity of nanomaterials in vivo. Here we have used dynamic light scattering （DLS） and transmission electron microscopy... |
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| SubjectTerms | 柠檬酸盐 牛血清白蛋白 电晕 纤维蛋白原 蛋白质 表面修饰 透射电子显微镜 金纳米粒子 |
| Title | Quantitative study of protein coronas on gold nano- particles with different surface modifications |
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