Influence of 12C6＋ ion irradiation on mutant avermitilis

• Published in: 中国物理C：英文版 Vol. 36; no. 11; pp. 1140 - 1144
• Main Author: 王曙阳 陈积红 李文建 梁剑平 薄永恒 马晓琪 刘敬
• Format: Journal Article
• Language: English
• Published: 2012
• Subjects: 照射; 突变体; 菌丝体生长; 菌株培养; 菌落形态; 重离子辐照; 阿维菌素B1a; 阿维链霉菌
• ISSN: 1674-1137

The effects of 12C＋6 ion irradiation on colony morphology and mycelia morphology, as well as on mutation rate have been studied in the B1a high-product strains （ZJAV-Y1-203） mutated by heavy ion irradiation and compared with that in the original strain （ZJAV-A-1）. After irradiating the rate of a straw hat colony type having a high ability of producing B1a in ZJAV-Y1-203 strains was higher than that found in ZJAV-A-1 strains. When strains were cultured in a liquid medium for 24 hours, the mycelium becoming thinner could be observed in all of the irradiated ZJAV- Y1-203 groups, but only in the ZJAV-A-1 groups irradiated at the dose of 50 Gy or more. The early growth of mycelium was inhibited in the ZJAV- Y1-203 group irradiated with a high dose. The highest positive mutation rate （23.5%） of ZJAV - Y1 - 203 was reached at the lower dose of 30 Gy while the highest positive mutation rate of 34.2% in ZJAV-A-1 appeared at 50 Gy. These results indicate that the effects of heavy ion irradiation still exist even in the mutated Streptomyces avermitilis, and only the dose is lower and the effects not so strong compared with the one that is first irradiated with optimized heavy ion doses. This is evidence of the one directional mutation being controlled by many more factors in a organism.