PRAME Gene Expression in Acute Leukemia and Its Clinical Significance

• Published in: 临床肿瘤和癌症研究：英文版 Vol. 9; no. 1; pp. 73 - 76
• Main Author: Kai Ding Xiao-ming Wang Rong Fu Er-bao Ruan Hui Liu Zong-hong Shao
• Format: Journal Article
• Language: English
• Published: 2012
• Subjects: E基因; PRAM; 临床意义; 基因表达水平; 急性白血病; 染色体核型; 淋巴细胞白血病; 白细胞计数
• ISSN: 1674-5361; 1868-324X

Objective To investigate the expression of the preferentially expressed antigen of melanoma （PRAME） gene in acute leukemia and its clinical significance. Methods The level of expressed PRAME mRNA in bone marrow mononuclear cells from 34 patients with acute leukemia （AL） and in 12 bone marrow samples from healthy volunteers was measured via RT-PCR. Correlation analyses between PRAME gene expression and the clinical characteristics （gender, age, white blood count, immunophenotype of leukemia, percentage of blast cells, and karyotype） of the patients were performed. Results The PRAME gene was expressed in 38.2% of all 34 patients, in 40.7% of the patients with acute myelogenous leukemia （AML, n=27）, and in 28.6% of the patients with acute lymphoblastic leukemia （ALL, n=7）, but was not expressed in the healthy volunteers. The difference in the expression levels between AML and ALL patients was statistically significant. The rate of gene expression was 80% in M~, 33.3% in M2, and 28.6% in M~. Gene expression was also found to be correlated with CDl5 and CD33 expression and abnormal karyotype, but not with age, gender; white blood count or percentage of blast cells. Conclusions The PRAME gene is highly expressed in acute leukemia and could be a useful marker to monitor minimal residual disease. This gene is also a candidate target for the immunotherapy of acute leukemia.