膀胱移行细胞癌来源exosome的制备及相关蛋白分子的鉴定
目的分离纯化膀胱移行细胞癌T24细胞来源的exosome,对其形态学和蛋白组成进行初步分析,为进一步利用exosome治疗膀胱移行细胞癌奠定基础。方法采用超滤和蔗糖密度梯度离心法分离T24细胞释放的exosome,电镜观察形态大小,SDS-PAGE分离蛋白并用软件分析,Western blot检测热休克蛋白70（HSP70）、细胞黏附分子（ICAM-1）、细胞角蛋白20（CK20）的表达。结果成功的分离出了exosome,其大小为30～90nm,类圆碟形小囊泡,Western blot证实exosome表达HSP70、ICAM-1和CK20分子。结论 T24细胞可以分泌exosome,并负载了...
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| Published in | Zhongliu fangzhi yanjiu Vol. 37; no. 6; pp. 636 - 639 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
2010
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| Subjects | |
| Online Access | Get full text |
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| Abstract | 目的分离纯化膀胱移行细胞癌T24细胞来源的exosome,对其形态学和蛋白组成进行初步分析,为进一步利用exosome治疗膀胱移行细胞癌奠定基础。方法采用超滤和蔗糖密度梯度离心法分离T24细胞释放的exosome,电镜观察形态大小,SDS-PAGE分离蛋白并用软件分析,Western blot检测热休克蛋白70（HSP70）、细胞黏附分子（ICAM-1）、细胞角蛋白20（CK20）的表达。结果成功的分离出了exosome,其大小为30～90nm,类圆碟形小囊泡,Western blot证实exosome表达HSP70、ICAM-1和CK20分子。结论 T24细胞可以分泌exosome,并负载了HSP70、ICAM-1等免疫相关蛋白,为肿瘤免疫治疗提供了理论基础。 |
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| AbstractList | 目的分离纯化膀胱移行细胞癌T24细胞来源的exosome,对其形态学和蛋白组成进行初步分析,为进一步利用exosome治疗膀胱移行细胞癌奠定基础。方法采用超滤和蔗糖密度梯度离心法分离T24细胞释放的exosome,电镜观察形态大小,SDS-PAGE分离蛋白并用软件分析,Western blot检测热休克蛋白70（HSP70）、细胞黏附分子（ICAM-1）、细胞角蛋白20（CK20）的表达。结果成功的分离出了exosome,其大小为30～90nm,类圆碟形小囊泡,Western blot证实exosome表达HSP70、ICAM-1和CK20分子。结论 T24细胞可以分泌exosome,并负载了HSP70、ICAM-1等免疫相关蛋白,为肿瘤免疫治疗提供了理论基础。 |
| Author | 张家模 吴小候 张尧 夏雨果 罗春丽 |
| AuthorAffiliation | 重庆医科大学附属第一医院泌尿外科,400016 重庆医科大学检验系 |
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| DocumentTitleAlternate | Preparation and Identification of exosome Derived from Transitional Cell Carcinoma of Bladder |
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| Notes | HSP7O 42-1241/R Exosome Transitional cell carcinoma of bladder CK20 R73-3 ICAM-1 Exosome; Transitional cell carcinoma of bladder; CK20; HSP7O; ICAM-1 |
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| Snippet | 目的分离纯化膀胱移行细胞癌T24细胞来源的exosome,对其形态学和蛋白组成进行初步分析,为进一步利用exosome治疗膀胱移行细胞癌奠定基础。方法采用超滤和蔗糖密度梯度离心法分离T24细胞释放的exosome,电镜观察形态大小,SDS-PAGE分离蛋白并用软件分析,Western... |
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| StartPage | 636 |
| SubjectTerms | CK20 exosome HSP70 ICAM-1 膀胱移行细胞癌 |
| Title | 膀胱移行细胞癌来源exosome的制备及相关蛋白分子的鉴定 |
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