Inhibition effect of some chemical anesthetics and hypothermia on the activity of glucose 6-phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vivo
Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık metabolizması üzerine olan olumsuz etkileri çoğunlukla dikkate alınmamaktadır. Bu çalışmada, Tranquil, tricaine methanesulfonate (MS-222) gibi kimyas...
Saved in:
Published in | Turkish journal of veterinary & animal sciences Vol. 28; no. 5; pp. 813 - 817 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
TÜBİTAK
2004
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık metabolizması üzerine olan olumsuz etkileri çoğunlukla dikkate alınmamaktadır.
Bu çalışmada, Tranquil, tricaine methanesulfonate (MS-222) gibi kimyasal anestezilerin ve soğukla muamelenin glikoz 6-fosfat dehidrogenaz (G6PDH) aktivitesi üzerine inhibisyon etkileri araştırıldı. Kontrol, başlangıç, l. saat ve 4. saatte enzim aktiviteleri sırasıyla soğukla muamelede 41,334 ±3,190 EU $(g Hb)^{-1}$, 34,586 ± 5,086 EU $(g Hb)^{-1}$, 40,768 ± 3,430 EU $(g Hb)^{-1}$ ve 41,957 ±3,157 EU $(g Hb)^{-1}$, Tranquilde 39,470 ± 2,250 EU $(g Hb)^{-1}$, 35,807 ± 2,000 EU $(g Hb)^{-1}$, 32,756 ± 7,822 EU $(g Hb)^{-1}$ ve 34,882 ± 3,452 EU $(g Hb)^{-1}$, MS-222'de ise 52,910 ± 2,490 EU $(g Hb)^{-1}$, 27,044 ± 2,750 EU $(g Hb)^{-1}$, 39,901 ± 4,477 EU $(g Hb)^{-1}$ ve 42,629 ± 2,593 EU $(g Hb)^{-1}$olarak tespit edildi.
Hypotermia, Tranquil ve MS-222, G6PDH aktivitesi üzerinde inhibisyon etkisi gösterdi. Hypotermia ve Tranquilin inhibisyon etkileri istatistiki olarak önemsiz (P > 0,05), fakat MS-222'nin inhibisyon etkisi başlangıçta istatistiki olarak oldukça yüksek bulundu (P < 0,01). MS-222'nin inhibisyon etkisi muameleden sonraki 1. ve 4. saatte azaldı (P < 0,05).
Many chemical anesthetics and drugs have been used in fish stripping, surgery and transportation, but their undesirable side effects on the body metabolism during treatment are not usually taken into consideration.
In this study, we investigated the inhibition effect of some chemical anesthetics, such as Tranquil and tricaine methanesulfonate (MS-222), and hypothermia on glucose 6-phosphate dehydrogenase (G6PDH) activity. The control, initial, l h and 4 h values were 41.334 ± 3.190 EU $(g Hb)^{-1}$, 34.586 ± 5.086 EU $(g Hb)^{-1}$, 40.768 ± 3.430 EU $(g Hb)^{-1}$ and 41.957 ± 3.157 EU $(g Hb)^{-1}$, respectively, in hypothermia. These values were 39.470 ± 2.250 EU (g Ho)'1, 35.807 ± 2.000 EU $(g Hb)^{-1}$, 32.756 ± 7.822 EU $(g Hb)^{-1}$ and 34.882 ± 3.452 EU (g Hb)-1 with Tranquil and 52.910 ± 2.490 EU $(g Hb)^{-1}$, 27.044 ± 2.750 EU (g Mb)'1, 39.901 ± 4.477 EU $(g Hb)^{-1}$ and 42.629 ± 2.593 EU $(g Hb)^{-1}$ with MS-222, respectively. Hypothermia, Tranquil and MS-222 showed inhibition effects on G6PDH activity. The inhibition effects of hypothermia and Tranquil were not statistically significant (P > 0.05), but MS-222 caused statistically quite high inhibition initially (P < 0.01). The inhibition effect of MS-222 diminished 1 h and 4 h after treatment (P < 0.05). |
---|---|
AbstractList | Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık metabolizması üzerine olan olumsuz etkileri çoğunlukla dikkate alınmamaktadır.
Bu çalışmada, Tranquil, tricaine methanesulfonate (MS-222) gibi kimyasal anestezilerin ve soğukla muamelenin glikoz 6-fosfat dehidrogenaz (G6PDH) aktivitesi üzerine inhibisyon etkileri araştırıldı. Kontrol, başlangıç, l. saat ve 4. saatte enzim aktiviteleri sırasıyla soğukla muamelede 41,334 ±3,190 EU $(g Hb)^{-1}$, 34,586 ± 5,086 EU $(g Hb)^{-1}$, 40,768 ± 3,430 EU $(g Hb)^{-1}$ ve 41,957 ±3,157 EU $(g Hb)^{-1}$, Tranquilde 39,470 ± 2,250 EU $(g Hb)^{-1}$, 35,807 ± 2,000 EU $(g Hb)^{-1}$, 32,756 ± 7,822 EU $(g Hb)^{-1}$ ve 34,882 ± 3,452 EU $(g Hb)^{-1}$, MS-222'de ise 52,910 ± 2,490 EU $(g Hb)^{-1}$, 27,044 ± 2,750 EU $(g Hb)^{-1}$, 39,901 ± 4,477 EU $(g Hb)^{-1}$ ve 42,629 ± 2,593 EU $(g Hb)^{-1}$olarak tespit edildi.
Hypotermia, Tranquil ve MS-222, G6PDH aktivitesi üzerinde inhibisyon etkisi gösterdi. Hypotermia ve Tranquilin inhibisyon etkileri istatistiki olarak önemsiz (P > 0,05), fakat MS-222'nin inhibisyon etkisi başlangıçta istatistiki olarak oldukça yüksek bulundu (P < 0,01). MS-222'nin inhibisyon etkisi muameleden sonraki 1. ve 4. saatte azaldı (P < 0,05).
Many chemical anesthetics and drugs have been used in fish stripping, surgery and transportation, but their undesirable side effects on the body metabolism during treatment are not usually taken into consideration.
In this study, we investigated the inhibition effect of some chemical anesthetics, such as Tranquil and tricaine methanesulfonate (MS-222), and hypothermia on glucose 6-phosphate dehydrogenase (G6PDH) activity. The control, initial, l h and 4 h values were 41.334 ± 3.190 EU $(g Hb)^{-1}$, 34.586 ± 5.086 EU $(g Hb)^{-1}$, 40.768 ± 3.430 EU $(g Hb)^{-1}$ and 41.957 ± 3.157 EU $(g Hb)^{-1}$, respectively, in hypothermia. These values were 39.470 ± 2.250 EU (g Ho)'1, 35.807 ± 2.000 EU $(g Hb)^{-1}$, 32.756 ± 7.822 EU $(g Hb)^{-1}$ and 34.882 ± 3.452 EU (g Hb)-1 with Tranquil and 52.910 ± 2.490 EU $(g Hb)^{-1}$, 27.044 ± 2.750 EU (g Mb)'1, 39.901 ± 4.477 EU $(g Hb)^{-1}$ and 42.629 ± 2.593 EU $(g Hb)^{-1}$ with MS-222, respectively. Hypothermia, Tranquil and MS-222 showed inhibition effects on G6PDH activity. The inhibition effects of hypothermia and Tranquil were not statistically significant (P > 0.05), but MS-222 caused statistically quite high inhibition initially (P < 0.01). The inhibition effect of MS-222 diminished 1 h and 4 h after treatment (P < 0.05). |
Author | HİSAR, Olcay ERDOĞAN, Orhan KOCAMAN, Esat Mahmut ÇİLTAŞ, Abdulkadir |
Author_xml | – fullname: HİSAR, Olcay – fullname: ÇİLTAŞ, Abdulkadir – fullname: ERDOĞAN, Orhan – fullname: KOCAMAN, Esat Mahmut |
BookMark | eNotjL1OwzAUhTMUibbwBgx3hCGSHSdxOqKKP6lSF5grx76uTRO7sp0iPxZvSBBM5zs6-s6qWDjvcFEsKSOkJLTqrotVjJ-EVKxuybL4fnPG9jZZ7wC1RpnAa4h-RJAGRyvFAMJhTAaTlXFmBSaf_dzDaAXM2owgZLIXm_KvfBwm6SNCW56Nj2cjEoJCk1XwR3RiXnTwIwRhXe-_IAU_JbjfO-mDyU6aKcKYTzbGB8CQkwle5oQRrIOLvfib4kqLIeLtf66Lj-en9-1rudu_vG0fd2WkvE1lI3rKe0pFrTeEcME2qlMN71A2gihNCKNcEbWRlWIV53XVMKko1zVD1KpFti7u_n6nQZx6Ox7OwY4i5APraNuwH_5Db4Q |
ContentType | Journal Article |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Veterinary Medicine |
DocumentTitleAlternate | Bazı kimyasal anestezikler ve soğukla muamelenin gökuşağı alabalığı (Oncorhynchus mykiss) eritrositlerindeki glikoz 6-Fosfat dehitrogenaz enzimi aktivitesi (in vivo) üzerine inhibisyon etkileri |
EndPage | 817 |
ExternalDocumentID | 38165 |
GroupedDBID | 123 29Q 2WC 53G ABDBF ABPTK ADBBV AENEX ALMA_UNASSIGNED_HOLDINGS B0M BAWUL C1A DIK E3Z EAP EMK EPL ESX GIZ KKE OBODZ OK1 RNS TUS XEI |
ID | FETCH-LOGICAL-s176t-5ab17b11a4f9007a39d8d578ec5a0df00317d0d9c2d32774253cd17f43eefd6e3 |
ISSN | 1300-0128 |
IngestDate | Tue Jan 05 18:08:19 EST 2021 |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 5 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-s176t-5ab17b11a4f9007a39d8d578ec5a0df00317d0d9c2d32774253cd17f43eefd6e3 |
Notes | TTAR |
PageCount | 5 |
ParticipantIDs | ulakbim_primary_38165 |
PublicationCentury | 2000 |
PublicationDate | 2004 |
PublicationDateYYYYMMDD | 2004-01-01 |
PublicationDate_xml | – year: 2004 text: 2004 |
PublicationDecade | 2000 |
PublicationTitle | Turkish journal of veterinary & animal sciences |
PublicationYear | 2004 |
Publisher | TÜBİTAK |
Publisher_xml | – name: TÜBİTAK |
References | 18. Çiftçi, M.: Bazı ilaçların insane eritrositlerinde bulunan glukoz 6 fosfat dehidrogenaz enzimi üzerine etkilerinin incelenmesi. Doctorate Thesis. Atatürk Uni. Graduate School of Natural and Applied Sciences. 1998. 17. Çiltaş, A., Erdoğan, O., Hisar, O., Çiftçi, M.: Effects of chloramine-t and CuS04 on enzyme activity of glucose 6- phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Israeli J. Aquacult- Bamidgeh., 2003; 55: 187-196. 10. Barroso, J.B., Peragön, J., Contreras-Jurado, C, Garcîa- Salguero, L., Corpas, F.J., Esteban, F.J., Peinado, M.A., De la Higuera, M., Lupianez, J.: Impact of starvation-refeeding on kinetics and protein expression of trout liver NADPH-production systems, Am. J. Physiol., 1998; 274: 1578-1587. 20. Evelo, C.T.A., Spooren, A.A.M.G., Bisschops, R.A.G., Baars, L.G.M., Neis, J.M.: Two mechanisms for toxic effects of hydroxylamines in human erythrocytes: involvement of free radicals and risk of potentiation. Blood Cells Mol. Dis., 1998; 24: 280-295. 11. Çiftçi, M., Küfrevioğlu Ö.İ., Gündoğdu M., Özmen, I.: Effects of some antibiotics on enzyme activity of glucose-6-phosphate dehydrogenase from human erythrocytes. Pharmacol. Res., 2000; 41: 109-113. 6. Miksicek, R.J., Towle, H.C.: Changes in the rats of synthesis and messenger RNA levels of hepatic glucose 6-phosphate dehydrogenases following induction by diet or thyroid hormone. J. Biol. Chem., 1982; 257: 11829-11835. 23. Kurtuluş, E.B., Tuncer, Ü.: A mouse model for evaluating the induction of liver glucose-6-phosphate dehydrogenase by halothane. Turk. J. Vet. Anim. Sci., 2000; 24: 511-515. 9. Barroso, J.B., Peragön, J., Garcia-Salguero, L., Higuera, M., Lupianez, J.: The influence of dietary protein on the kinetics of NADPH-production systems in various tissues of rainbow trout, Aquaculture, 1994; 124: 47-59. 21. Gabryelak, T., Zalesna, G., Roche, H., Peres, G.: The effect of MS 222 an anaesthetic on the peroxide metabolism enzymes in erythrocytes of freshwater and marine fish species. Comp. Biochem. Physiol. Part C: Comp. Pharma. Toxicol., 1989; 92: 5-8 8. Kletzien, R.F., Prosko, C.R., Stumpo, DJ. McClung, K., Dreher, K.L.: Molecular cloning of DNA sequences complementary to rat liver glucose 6-phosphate dehydrogenase mRNA. Nutritional regulation of mRNA levels, J. Biol. Chem., 1985; 260: 5621-5624. 24. Kumar, P., Prasad, A.K., Dutta, K.K.: Steroiddgenic alterations in testes and sera of rats exposed to trichloroethylene (TCE) by inhalation. Human Exp. Toxicol., 2000: 19: 117-121. 13. Ninfali P., Orsenigo T., Barociani L., Rapa, S.: Rapid purification of glucose-6-phosphate dehydrogenase from mammal's erythrocyte. Prep. Biochem., 1990; 20: 297-309. 7. Barroso, J. B., Peragön, J., Garcîa-Salguero, L, Higuera, M., Lupiânez, J.: Variations in the kinetic behaviour of the NADPH- production systems in different tissues of the trout when fed on an amino-acid-based diet at different frequencies. Int. J. Biochem. Cell Biol., 1999:31:277-290. 3. Weksler B.B., Moore A., Tepler, J.: Hematology. In: T.E. Andreoli, C.CJ. Carpenter, F. Plum and L.H. Smith, Eds. Cecil essentials of medicine. 2nd ed. Philadelphia: WB Saunders Company. 1990; 341-363. 5. Tomlinson, J.E., Nakayama, R., Holten, D.: Repression of pentose phosphate pathway dehydrogenase synthesis and mRNA by dietary fat in rats. J. Nutr., 1988; 118: 408-415. 19. Çiftçi, M., Küfrevioğlu, Ö.İ., Gündoğdu, M., Özmen, L Effects of some antibiotics on enzyme activity of glucose-6-phosphate dehydrogenase from human erythrocytes. Pharmacol. Res., 2000;41: 107-111. 22. Fabacher, D.L.: Hepatic microsomes from freshwater fish-II. reduction of benzo(a)pyrene metabolism by the fish anesthetics quinaldine sulfate and tricaine. Comp. Biochem. Physiol. Part C: Comp. Pharma. Toxicol., 1982; 73: 285-288. 4. Ogawa, K., Solt, D.B., Farber, E.: Phenotypic diversity as an early property of putative preneoplastic hepatocyte populations in liver carcinogenesis. Cancer Res., 1980; 40: 725-733. 16. Winzer, K., Van Noorden, C.J.F., Kohler.A.: Glucose-6-phosphate dehydrogenase: the key to sex-related xenobiotic toxicity in hepatocytes of European flounder (Platichthys fiesus L). Aqua. Toxicol., 2002; 56: 275-288. 2. Deutsch J: GIucose-6-phosphate dehydrogenase. In: Bergmeyer, H.U., Bergmeyer, J. Eds. Methods of enzymatic analysis. Verlagsgerellschaff, VCH. 1983; 3: 190-196. 1. Kuo, W., Lin, J., Tang, T.K.: Human glucose-6-phosphate dehydrogenase (G6PD) gene transforms nih 3t3 cells and induces tumors in nude mice. Int. J. Cancer, 2000; 85: 857-864. 12. Summerfelt, R.C., Smith, L.S.: Anesthesia, Surgery, and Related Techniques: In Schreck, C.B., Moyle, P.B., Eds. Methods for Fish Biology. AFS, Bethesta, Maryland, USA. 1990; 213-272. 14. Delgado, C, Tejedor, C, Luquue, J.: Partial purification of glucose-6-phosphate dehydrogenase from rat erythrocyte haemolysate by partitioning in aqueous two-phase system. J. Chromatogr., 1990; 498: 159-168. 15. Beutler E: Red cell metabolism manual of biocheWal methods. London: Academic Press 1971. |
References_xml | |
SSID | ssj0023460 |
Score | 1.6352609 |
Snippet | Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık... |
SourceID | ulakbim |
SourceType | Open Access Repository |
StartPage | 813 |
SubjectTerms | alyuvarlar anaesthetics anestetikler Animal Physiology and Biochemistry (Excluding Nutrition) Aquaculture(Animals) Aquatic Biology and Ecology engelleme enzim aktivitesi enzyme activity erythrocytes glucose-6-phosphate dehydrogenase glukoz-6-fosfat dehidrogenaz gökkuşağı alabalığı Hayvan Fizyolojisi ve Biyokimyası (Beslenme Dışında) hipotermi hypothermia inhibition Oncorhynchus mykiss rainbow trout Sucul Biyoloji ve Ekoloji Sucul Kültür(Hayvanlar) Veterinary Pharmacology and Anaestesiology Veteriner Farmakoloji ve Anesteziyoloji |
Title | Inhibition effect of some chemical anesthetics and hypothermia on the activity of glucose 6-phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vivo |
Volume | 28 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFLWq8cILgjEEDJAfmASKgpLm-zHtOhXGqAQZ2tvk2I4StUmqfFQK_4rfwZ_iOnabDIEEvESW28RR7onvvfY5Nwi95sQGv8eJznyYAu2AOjphNNYD7ltG4jmJ7wuh8NUnd3ltf7hxbiaTHyPWUtvE7-i33-pK_seq0Ad2FSrZf7Ds4aLQAW2wLxzBwnD8Kxu_L9Is7jlXipchQr-6zLlGhzoAMO2n_FCMOe22veYqz4jaJ-jLaewUM2PPYHf1bVrW2xQiUY3xtGNVCXcBHk_qUSq5K6Q1laA19xVLIYtNu4Kmba3lHUSltVhv4FUnvsNAu6bnfWm7bFeOw-GordaipNKofsVO8HOkSFigkhRZvtdt0oHvuBRB8Mz4EvY8jtWGDlwgsfXve_L3j1F4NnfOgp7yGcas3awJOPFDGvH5fCX-GCzCnu2wqtLhZblczcEh9f2LmjTaFUnztrmzSjKsj0Zi1GA-k8NG4eVosreEqt5U4nTlDab-CPXOaGr3pWZ2HyVIxekvtbrFLqwoeGAbFsy598LZ-ezikP5bttKsq1Eh42o3ZB1n-SiiiR6iByoVwaHE1SM04cUxOv56eP74SvEuHqPvA9KwRBouEyyQhvdIwyOkQZvhEdIwnAZNvEeaOFkhDY-Qhu8gDQukYYU03CMNvxnjDEucvcVjlOGswAJlJ-j6YhHNl7r62Idem57b6A6JTS82TWInAcStxAqYz8CdcOoQgyXC-XjMYAGdMmsKOcvUsSgzvcS2OE-Yy60n6KgoC_4UYZcyZ-q7JDDixDZiN3b9IAkI5N4xhUiNPkMn6sHfbmVJl9vecM__0H-K7ku-llh4e4GOmqrlLyEUbeJXysY_AUwul8M |
link.rule.ids | 315,783,787,4033 |
linkProvider | EBSCOhost |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Inhibition+effect+of+some+chemical+anesthetics+and+hypothermia+on+the+activity+of+glucose+6-phosphate+dehydrogenase+from+rainbow+trout+%28Oncorhynchus+mykiss%29+erythrocytes+in+vivo&rft.jtitle=Turkish+journal+of+veterinary+%26+animal+sciences&rft.au=H%C4%B0SAR%2C+Olcay&rft.au=%C3%87%C4%B0LTA%C5%9E%2C+Abdulkadir&rft.au=ERDO%C4%9EAN%2C+Orhan&rft.au=KOCAMAN%2C+Esat+Mahmut&rft.date=2004&rft.pub=T%C3%9CB%C4%B0TAK&rft.issn=1300-0128&rft.volume=28&rft.issue=5&rft.spage=813&rft.epage=817&rft.externalDocID=38165 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1300-0128&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1300-0128&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1300-0128&client=summon |