Inhibition effect of some chemical anesthetics and hypothermia on the activity of glucose 6-phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vivo

Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık metabolizması üzerine olan olumsuz etkileri çoğunlukla dikkate alınmamaktadır. Bu çalışmada, Tranquil, tricaine methanesulfonate (MS-222) gibi kimyas...

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Published inTurkish journal of veterinary & animal sciences Vol. 28; no. 5; pp. 813 - 817
Main Authors HİSAR, Olcay, ÇİLTAŞ, Abdulkadir, ERDOĞAN, Orhan, KOCAMAN, Esat Mahmut
Format Journal Article
LanguageEnglish
Published TÜBİTAK 2004
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Abstract Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık metabolizması üzerine olan olumsuz etkileri çoğunlukla dikkate alınmamaktadır. Bu çalışmada, Tranquil, tricaine methanesulfonate (MS-222) gibi kimyasal anestezilerin ve soğukla muamelenin glikoz 6-fosfat dehidrogenaz (G6PDH) aktivitesi üzerine inhibisyon etkileri araştırıldı. Kontrol, başlangıç, l. saat ve 4. saatte enzim aktiviteleri sırasıyla soğukla muamelede 41,334 ±3,190 EU $(g Hb)^{-1}$, 34,586 ± 5,086 EU $(g Hb)^{-1}$, 40,768 ± 3,430 EU $(g Hb)^{-1}$ ve 41,957 ±3,157 EU $(g Hb)^{-1}$, Tranquilde 39,470 ± 2,250 EU $(g Hb)^{-1}$, 35,807 ± 2,000 EU $(g Hb)^{-1}$, 32,756 ± 7,822 EU $(g Hb)^{-1}$ ve 34,882 ± 3,452 EU $(g Hb)^{-1}$, MS-222'de ise 52,910 ± 2,490 EU $(g Hb)^{-1}$, 27,044 ± 2,750 EU $(g Hb)^{-1}$, 39,901 ± 4,477 EU $(g Hb)^{-1}$ ve 42,629 ± 2,593 EU $(g Hb)^{-1}$olarak tespit edildi. Hypotermia, Tranquil ve MS-222, G6PDH aktivitesi üzerinde inhibisyon etkisi gösterdi. Hypotermia ve Tranquilin inhibisyon etkileri istatistiki olarak önemsiz (P > 0,05), fakat MS-222'nin inhibisyon etkisi başlangıçta istatistiki olarak oldukça yüksek bulundu (P < 0,01). MS-222'nin inhibisyon etkisi muameleden sonraki 1. ve 4. saatte azaldı (P < 0,05). Many chemical anesthetics and drugs have been used in fish stripping, surgery and transportation, but their undesirable side effects on the body metabolism during treatment are not usually taken into consideration. In this study, we investigated the inhibition effect of some chemical anesthetics, such as Tranquil and tricaine methanesulfonate (MS-222), and hypothermia on glucose 6-phosphate dehydrogenase (G6PDH) activity. The control, initial, l h and 4 h values were 41.334 ± 3.190 EU $(g Hb)^{-1}$, 34.586 ± 5.086 EU $(g Hb)^{-1}$, 40.768 ± 3.430 EU $(g Hb)^{-1}$ and 41.957 ± 3.157 EU $(g Hb)^{-1}$, respectively, in hypothermia. These values were 39.470 ± 2.250 EU (g Ho)'1, 35.807 ± 2.000 EU $(g Hb)^{-1}$, 32.756 ± 7.822 EU $(g Hb)^{-1}$ and 34.882 ± 3.452 EU (g Hb)-1 with Tranquil and 52.910 ± 2.490 EU $(g Hb)^{-1}$, 27.044 ± 2.750 EU (g Mb)'1, 39.901 ± 4.477 EU $(g Hb)^{-1}$ and 42.629 ± 2.593 EU $(g Hb)^{-1}$ with MS-222, respectively. Hypothermia, Tranquil and MS-222 showed inhibition effects on G6PDH activity. The inhibition effects of hypothermia and Tranquil were not statistically significant (P > 0.05), but MS-222 caused statistically quite high inhibition initially (P < 0.01). The inhibition effect of MS-222 diminished 1 h and 4 h after treatment (P < 0.05).
AbstractList Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık metabolizması üzerine olan olumsuz etkileri çoğunlukla dikkate alınmamaktadır. Bu çalışmada, Tranquil, tricaine methanesulfonate (MS-222) gibi kimyasal anestezilerin ve soğukla muamelenin glikoz 6-fosfat dehidrogenaz (G6PDH) aktivitesi üzerine inhibisyon etkileri araştırıldı. Kontrol, başlangıç, l. saat ve 4. saatte enzim aktiviteleri sırasıyla soğukla muamelede 41,334 ±3,190 EU $(g Hb)^{-1}$, 34,586 ± 5,086 EU $(g Hb)^{-1}$, 40,768 ± 3,430 EU $(g Hb)^{-1}$ ve 41,957 ±3,157 EU $(g Hb)^{-1}$, Tranquilde 39,470 ± 2,250 EU $(g Hb)^{-1}$, 35,807 ± 2,000 EU $(g Hb)^{-1}$, 32,756 ± 7,822 EU $(g Hb)^{-1}$ ve 34,882 ± 3,452 EU $(g Hb)^{-1}$, MS-222'de ise 52,910 ± 2,490 EU $(g Hb)^{-1}$, 27,044 ± 2,750 EU $(g Hb)^{-1}$, 39,901 ± 4,477 EU $(g Hb)^{-1}$ ve 42,629 ± 2,593 EU $(g Hb)^{-1}$olarak tespit edildi. Hypotermia, Tranquil ve MS-222, G6PDH aktivitesi üzerinde inhibisyon etkisi gösterdi. Hypotermia ve Tranquilin inhibisyon etkileri istatistiki olarak önemsiz (P > 0,05), fakat MS-222'nin inhibisyon etkisi başlangıçta istatistiki olarak oldukça yüksek bulundu (P < 0,01). MS-222'nin inhibisyon etkisi muameleden sonraki 1. ve 4. saatte azaldı (P < 0,05). Many chemical anesthetics and drugs have been used in fish stripping, surgery and transportation, but their undesirable side effects on the body metabolism during treatment are not usually taken into consideration. In this study, we investigated the inhibition effect of some chemical anesthetics, such as Tranquil and tricaine methanesulfonate (MS-222), and hypothermia on glucose 6-phosphate dehydrogenase (G6PDH) activity. The control, initial, l h and 4 h values were 41.334 ± 3.190 EU $(g Hb)^{-1}$, 34.586 ± 5.086 EU $(g Hb)^{-1}$, 40.768 ± 3.430 EU $(g Hb)^{-1}$ and 41.957 ± 3.157 EU $(g Hb)^{-1}$, respectively, in hypothermia. These values were 39.470 ± 2.250 EU (g Ho)'1, 35.807 ± 2.000 EU $(g Hb)^{-1}$, 32.756 ± 7.822 EU $(g Hb)^{-1}$ and 34.882 ± 3.452 EU (g Hb)-1 with Tranquil and 52.910 ± 2.490 EU $(g Hb)^{-1}$, 27.044 ± 2.750 EU (g Mb)'1, 39.901 ± 4.477 EU $(g Hb)^{-1}$ and 42.629 ± 2.593 EU $(g Hb)^{-1}$ with MS-222, respectively. Hypothermia, Tranquil and MS-222 showed inhibition effects on G6PDH activity. The inhibition effects of hypothermia and Tranquil were not statistically significant (P > 0.05), but MS-222 caused statistically quite high inhibition initially (P < 0.01). The inhibition effect of MS-222 diminished 1 h and 4 h after treatment (P < 0.05).
Author HİSAR, Olcay
ERDOĞAN, Orhan
KOCAMAN, Esat Mahmut
ÇİLTAŞ, Abdulkadir
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DocumentTitleAlternate Bazı kimyasal anestezikler ve soğukla muamelenin gökuşağı alabalığı (Oncorhynchus mykiss) eritrositlerindeki glikoz 6-Fosfat dehitrogenaz enzimi aktivitesi (in vivo) üzerine inhibisyon etkileri
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17. Çiltaş, A., Erdoğan, O., Hisar, O., Çiftçi, M.: Effects of chloramine-t and CuS04 on enzyme activity of glucose 6- phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Israeli J. Aquacult- Bamidgeh., 2003; 55: 187-196.
10. Barroso, J.B., Peragön, J., Contreras-Jurado, C, Garcîa- Salguero, L., Corpas, F.J., Esteban, F.J., Peinado, M.A., De la Higuera, M., Lupianez, J.: Impact of starvation-refeeding on kinetics and protein expression of trout liver NADPH-production systems, Am. J. Physiol., 1998; 274: 1578-1587.
20. Evelo, C.T.A., Spooren, A.A.M.G., Bisschops, R.A.G., Baars, L.G.M., Neis, J.M.: Two mechanisms for toxic effects of hydroxylamines in human erythrocytes: involvement of free radicals and risk of potentiation. Blood Cells Mol. Dis., 1998; 24: 280-295.
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Snippet Balıkların sağımında, cerrahisinde ve taşınmasında birçok kimyasal anestezik ve ilaçlar kullanılmaktadır. Fakat muamele esnasında anasteziklerin balık...
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SourceType Open Access Repository
StartPage 813
SubjectTerms alyuvarlar
anaesthetics
anestetikler
Animal Physiology and Biochemistry (Excluding Nutrition)
Aquaculture(Animals)
Aquatic Biology and Ecology
engelleme
enzim aktivitesi
enzyme activity
erythrocytes
glucose-6-phosphate dehydrogenase
glukoz-6-fosfat dehidrogenaz
gökkuşağı alabalığı
Hayvan Fizyolojisi ve Biyokimyası (Beslenme Dışında)
hipotermi
hypothermia
inhibition
Oncorhynchus mykiss
rainbow trout
Sucul Biyoloji ve Ekoloji
Sucul Kültür(Hayvanlar)
Veterinary Pharmacology and Anaestesiology
Veteriner Farmakoloji ve Anesteziyoloji
Title Inhibition effect of some chemical anesthetics and hypothermia on the activity of glucose 6-phosphate dehydrogenase from rainbow trout (Oncorhynchus mykiss) erythrocytes in vivo
Volume 28
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