NEW PERSPECTIVES ON THE DETERMINATION OF PHOSPHATASE ACTIVITY IN ECTOMYCORRHIZAE OF NOTHOFAGUS OBLIQUA FORESTS IN SOUTHERN CHILE

• Published in: Gayana. Botánica. Vol. 60; no. 1; pp. 41 - 46
• Main Authors: Alvarez, Maricel, Härtel, Steffen, Godoy, Roberto, Heyser, Wolfgang
• Format: Journal Article
• Language: English; Portuguese
• Published: Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción 2003
• Subjects: PLANT SCIENCES; quantitative fluorescence microscopy; ELF-97; phosphatase activity; microscopía cuantitiva de fluorescencia; ectomicorrizas; Actividad fosfatasa; Confocal microscopy; ectomycorrhizae of Nothofagus obliqua; microscopía confocal

We present a method to localise and quantify surface-bound phosphatase activity (SBPA) in mycorrhizal fungi and mycorrhizae of Nothofagus obliqua (Mirb.) Oerst., using image processed confocal fluorescent microscopy. ELF-97 is a hydrophilic substrate which turns into a strongly fluorescent precipita...