Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
Se reporta la evaluación del sistema DAVIH-BLOT (Laboratorios DAVIH, Cuba) frente a los paneles de proficiencia enviados al Laboratorio Nacional de Referencia del SIDA, por la Organización Mundial de la Salud (OMS), para el aseguramiento de la calidad en el diagnóstico de anticuerpos al VIH en los a...
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Published in | Revista cubana de medicina tropical Vol. 49; no. 1; pp. 28 - 31 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | Portuguese |
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Centro Nacional de Información de Ciencias Médicas
01.04.1997
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Abstract | Se reporta la evaluación del sistema DAVIH-BLOT (Laboratorios DAVIH, Cuba) frente a los paneles de proficiencia enviados al Laboratorio Nacional de Referencia del SIDA, por la Organización Mundial de la Salud (OMS), para el aseguramiento de la calidad en el diagnóstico de anticuerpos al VIH en los años 1989, 1991 y 1993, así como la comparación del sistema con los de las firmas DuPont y Diagnostic Pasteur en un grupo de 467 sueros positivos por ELISA. En el estudio de las muestras de la OMS el sistema demostró ser altamente sensible y específico. Al compararlos con los estuches HIV-1 Western blot IgG versión 1.2 (DuPont Company, EE.UU.) y New LAV-blot 1 (Diagnostic Pasteur, Francia) tuvo una coincidencia del 100 y 97 %, respectivamente. |
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AbstractList | Se reporta la evaluación del sistema DAVIH-BLOT (Laboratorios DAVIH, Cuba) frente a los paneles de proficiencia enviados al Laboratorio Nacional de Referencia del SIDA, por la Organización Mundial de la Salud (OMS), para el aseguramiento de la calidad en el diagnóstico de anticuerpos al VIH en los años 1989, 1991 y 1993, así como la comparación del sistema con los de las firmas DuPont y Diagnostic Pasteur en un grupo de 467 sueros positivos por ELISA. En el estudio de las muestras de la OMS el sistema demostró ser altamente sensible y específico. Al compararlos con los estuches HIV-1 Western blot IgG versión 1.2 (DuPont Company, EE.UU.) y New LAV-blot 1 (Diagnostic Pasteur, Francia) tuvo una coincidencia del 100 y 97 %, respectivamente. |
Author | RUIBAL BRUNET, IGNACIO PÉREZ GUEVARA, MARÍA TERESA LOBAINA BATELEMY, LEONOR CRUZ SUI, OTTO IZQUIERDO MÁRQUEZ, MARICELA SILVA CABRERA, ELADIO |
AuthorAffiliation | Centro de Investigaciones Científicas de la Defensa Civil. Laboratorio de Investigaciones del SIDA |
AuthorAffiliation_xml | – name: Centro de Investigaciones Científicas de la Defensa Civil. Laboratorio de Investigaciones del SIDA |
Author_xml | – sequence: 1 givenname: OTTO surname: CRUZ SUI fullname: CRUZ SUI, OTTO organization: Centro de Investigaciones Científicas de la Defensa Civil. Laboratorio de Investigaciones del SIDA – sequence: 2 givenname: MARÍA TERESA surname: PÉREZ GUEVARA fullname: PÉREZ GUEVARA, MARÍA TERESA – sequence: 3 givenname: MARICELA surname: IZQUIERDO MÁRQUEZ fullname: IZQUIERDO MÁRQUEZ, MARICELA – sequence: 4 givenname: LEONOR surname: LOBAINA BATELEMY fullname: LOBAINA BATELEMY, LEONOR – sequence: 5 givenname: IGNACIO surname: RUIBAL BRUNET fullname: RUIBAL BRUNET, IGNACIO – sequence: 6 givenname: ELADIO surname: SILVA CABRERA fullname: SILVA CABRERA, ELADIO |
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Keywords | ESTUDIOS DE EVALUACION WESTERN BLOTTING AIDS SERODIAGNOSIS ANTICUERPOS HIV BLOTTING WESTERN SERODIAGNOSTICO DEL SIDA HIV ANTIBODIES EVALUATION STUDIES |
Language | Portuguese |
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References | Glassman, AB; Sherrill, T; Paolini, J 1990; 20 Fang, CT; Darr, F; Kleinman, S; Wehling, RH; Dodd, RY 1986; 26 1990; 65 Healey, DS; Maskill, WJ; Howard, TS; Armstrong, VA; Bolton, WV; Cooper, GJ; et, al 1992; 6 Sarngadharan, MG; Popovic, M; Bruch, L; Shupbach, J; Gallo, RC 1984; 224 Kleinman, S 1990; 114 Dodd, RY; Fang, CT 1990; 114 Lundberg, GD 1988; 260 Downie, JC; Howard, R; Bowcock, B; Cunningham, AL 1989; 26 Schupbach, J; Popovic, M; Gilden, RV; Gonda, MA; Sarngadharan, MG 1984; 224 Blomberg, J; Klasse, PJ 1988; 26 Esteban, JI; Shih, JW; Tai, CC; Bodner, AJ; Kay, JW; Alter, HJ 1985; 2 1989 1992; 41 Constantine, NT; Callaham, JD; Watts, DM 1991 |
References_xml | – volume: 26 start-page: 208 year: 1986 end-page: 9 article-title: Relative especificity of enzyme linked immunosorbent assays for antibodies to Human T cell lymphotropic virus type III, and their relationship to Western blotting publication-title: Transfusion contributor: fullname: Fang, CT; Darr, F; Kleinman, S; Wehling, RH; Dodd, RY – volume: 2 start-page: 1083 year: 1985 end-page: 6 article-title: Importance of Western blot analysis in predicting infectivity of anti HTLV-III/LAV positive blood publication-title: Lancet contributor: fullname: Esteban, JI; Shih, JW; Tai, CC; Bodner, AJ; Kay, JW; Alter, HJ – volume: 6 start-page: 629 issue: 7 year: 1992 end-page: 33 article-title: HIV-1 Western blot: development and assessment of testing to resolve indeterminate reactivity publication-title: AIDS contributor: fullname: Healey, DS; Maskill, WJ; Howard, TS; Armstrong, VA; Bolton, WV; Cooper, GJ; et, al – volume: 26 start-page: 106 issue: 1 year: 1988 end-page: 10 article-title: Specificities and sensitivities of three systems for determination of antibodies to human immunodeficiency virus by electrophoretic immunoblotting publication-title: J Clin Microbiol contributor: fullname: Blomberg, J; Klasse, PJ – volume: 260 start-page: 674 issue: 5 year: 1988 end-page: 9 article-title: Serological diagnosis of human immunodeficiency virus infection by Western blot testing publication-title: JAMA contributor: fullname: Lundberg, GD – volume: 224 start-page: 503 year: 1984 end-page: 5 article-title: Serological analysis of a subgroup of human retroviruses (HTLV-III) associated with AIDS publication-title: Science contributor: fullname: Schupbach, J; Popovic, M; Gilden, RV; Gonda, MA; Sarngadharan, MG – volume: 41 start-page: 1 year: 1992 end-page: 9 article-title: Testing for antibodies to human immunodeficiency virus type 2 in the United States publication-title: MMWR – volume: 65 start-page: 281 issue: 37 year: 1990 end-page: 8 article-title: World Health Organization Proposed WHO criteria for interpreting results from Western blot assays for HIV-1, HIV-2, and HTLV-1/HTLV-II publication-title: Wkly Epidemiol Rec – volume: 224 start-page: 506 year: 1984 end-page: 8 article-title: Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS publication-title: Science contributor: fullname: Sarngadharan, MG; Popovic, M; Bruch, L; Shupbach, J; Gallo, RC – volume: 26 start-page: 291 year: 1989 end-page: 304 article-title: HIV-1 antibody testing strategy: evaluation of ELISA screening and Western blot profiles in a mixed low risk/high risk patient population publication-title: J Virol Methods contributor: fullname: Downie, JC; Howard, R; Bowcock, B; Cunningham, AL – volume: 20 start-page: 343 issue: 5 year: 1990 end-page: 6 article-title: Human immunodeficiency virus Western blot tests: comparisons and considerations publication-title: Ann Clin Lab Sci contributor: fullname: Glassman, AB; Sherrill, T; Paolini, J – start-page: 1 year: 1989 end-page: 13 publication-title: Sistema para la detección de anticuerpos al virus de la inmunodeficiencia humana tipo 1 (VIH 1) en suero o plasma.: Manual de Instrucciones. – volume: 114 start-page: 298 year: 1990 end-page: 303 article-title: The significance of HIV-1 indeterminate Western blot results in blood donor populations publication-title: Arch Pathol Lab Med contributor: fullname: Kleinman, S – volume: 114 start-page: 240 year: 1990 end-page: 5 article-title: The Western immunoblot procedure for HIV antibodies and its interpretation publication-title: Arch Pathol Lab Med contributor: fullname: Dodd, RY; Fang, CT – start-page: 62 year: 1991 end-page: 6 publication-title: Pruebas para la detección del VIH y control de calidad: Guía personal de laboratorio. contributor: fullname: Constantine, NT; Callaham, JD; Watts, DM |
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Title | Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1 |
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