上海市杨浦区学龄前儿童犬弓首蛔虫的感染情况及环境中犬弓首蛔虫的存在情况调查
R532; 目的·调查上海市杨浦区学龄前儿童犬弓首蛔虫的血清阳性率以及犬弓首蛔虫在公共环境中的存在情况.方法·采用横断面研究设计,对上海市杨浦区7家幼儿园的789名大班儿童进行了犬弓首蛔虫感染相关的问卷调查,用酶联免疫吸附法检测其中605名儿童血清中犬弓首蛔虫特异性IgG抗体的阳性率.并在这些幼儿园附近的小区和公园共采集67份土壤样品和69份犬粪便样品,提取基因组DNA,用PCR扩增法检测犬弓首蛔虫核糖体DNA第2内转录间隔区(internal transcribed spacer 2,ITS-2)序列基因.结果·上海市杨浦区儿童犬弓首蛔虫特异性抗体的血清阳性率为1.0％.土壤和犬粪便样品的犬...
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| Published in | 上海交通大学学报（医学版） Vol. 39; no. 12; pp. 1451 - 1455 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
上海交通大学医学院附属新华医院眼科,上海,200092%中国农业科学院上海兽医研究所,上海,200241
28.12.2019
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-8115 |
| DOI | 10.3969/j.issn.1674-8115.2019.12.018 |
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| Abstract | R532; 目的·调查上海市杨浦区学龄前儿童犬弓首蛔虫的血清阳性率以及犬弓首蛔虫在公共环境中的存在情况.方法·采用横断面研究设计,对上海市杨浦区7家幼儿园的789名大班儿童进行了犬弓首蛔虫感染相关的问卷调查,用酶联免疫吸附法检测其中605名儿童血清中犬弓首蛔虫特异性IgG抗体的阳性率.并在这些幼儿园附近的小区和公园共采集67份土壤样品和69份犬粪便样品,提取基因组DNA,用PCR扩增法检测犬弓首蛔虫核糖体DNA第2内转录间隔区(internal transcribed spacer 2,ITS-2)序列基因.结果·上海市杨浦区儿童犬弓首蛔虫特异性抗体的血清阳性率为1.0％.土壤和犬粪便样品的犬弓首蛔虫DNA阳性率分别为4.5％和27.5％.问卷调查显示90.0％以上的儿童洗手习惯好、家长预防意识强;6.8％的儿童家中有狗,77.8％的狗接受过驱虫治疗;83.5％的儿童家附近有流浪狗,但仅2.0％的儿童接触过流浪狗.结论·该地区学龄前儿童犬弓首蛔虫特异性抗体的血清阳性率较低,好的卫生习惯与预防意识可能是主要原因.但环境中仍存在较高的犬弓首蛔虫感染风险,因此推广实施预防性的公共卫生措施对我国学龄前儿童眼弓蛔虫病的早期防治有重大意义. |
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| AbstractList | R532; 目的·调查上海市杨浦区学龄前儿童犬弓首蛔虫的血清阳性率以及犬弓首蛔虫在公共环境中的存在情况.方法·采用横断面研究设计,对上海市杨浦区7家幼儿园的789名大班儿童进行了犬弓首蛔虫感染相关的问卷调查,用酶联免疫吸附法检测其中605名儿童血清中犬弓首蛔虫特异性IgG抗体的阳性率.并在这些幼儿园附近的小区和公园共采集67份土壤样品和69份犬粪便样品,提取基因组DNA,用PCR扩增法检测犬弓首蛔虫核糖体DNA第2内转录间隔区(internal transcribed spacer 2,ITS-2)序列基因.结果·上海市杨浦区儿童犬弓首蛔虫特异性抗体的血清阳性率为1.0％.土壤和犬粪便样品的犬弓首蛔虫DNA阳性率分别为4.5％和27.5％.问卷调查显示90.0％以上的儿童洗手习惯好、家长预防意识强;6.8％的儿童家中有狗,77.8％的狗接受过驱虫治疗;83.5％的儿童家附近有流浪狗,但仅2.0％的儿童接触过流浪狗.结论·该地区学龄前儿童犬弓首蛔虫特异性抗体的血清阳性率较低,好的卫生习惯与预防意识可能是主要原因.但环境中仍存在较高的犬弓首蛔虫感染风险,因此推广实施预防性的公共卫生措施对我国学龄前儿童眼弓蛔虫病的早期防治有重大意义. |
| Author | 张翔 李旌 卢祖鹏 赵培泉 米荣升 |
| AuthorAffiliation | 上海交通大学医学院附属新华医院眼科,上海,200092%中国农业科学院上海兽医研究所,上海,200241 |
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| Author_FL | ZHAO Pei-quan LI Jing MI Rong-sheng LU Zu-peng ZHANG Xiang |
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| Keywords | 眼弓蛔虫病 环境 血清流行病学 犬弓首蛔虫 学龄前儿童 |
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| Title | 上海市杨浦区学龄前儿童犬弓首蛔虫的感染情况及环境中犬弓首蛔虫的存在情况调查 |
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