对糖尿病肾脏疾病湿热证患者与正常人群尿液外泌体微RNA差异表达的研究
R259; 目的 通过外泌体小RNA测序技术,比较慢性肾脏病3～4期糖尿病肾脏疾病(DKD)湿热证患者与正常人群尿液外泌体微RNA(miRNA)的差异表达.方法 2021年4月—2022年4月于安徽中医药大学第一附属医院招募受试者,DKD湿热证患者40例,健康体检正常者40名.2组分别任选10名/例受试者,对尿液外泌体进行miRNA高通量测序,以log2(Fold Change)绝对值≥1及q值<0.05为条件筛选出所有差异表达miRNA,然后进一步筛选出10个miRNA进行验证,筛选条件为:①2组样本中,至少1组组内平均表达量(TPM)>2;②log2(Fold Change)绝...
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| Published in | 北京中医药大学学报 Vol. 45; no. 12; pp. 1205 - 1212 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
安徽中医药大学第一附属医院肾病科 合肥 230031
01.12.2022
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1006-2157 |
| DOI | 10.3969/j.issn.1006-2157.2022.12.004 |
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| Abstract | R259; 目的 通过外泌体小RNA测序技术,比较慢性肾脏病3～4期糖尿病肾脏疾病(DKD)湿热证患者与正常人群尿液外泌体微RNA(miRNA)的差异表达.方法 2021年4月—2022年4月于安徽中医药大学第一附属医院招募受试者,DKD湿热证患者40例,健康体检正常者40名.2组分别任选10名/例受试者,对尿液外泌体进行miRNA高通量测序,以log2(Fold Change)绝对值≥1及q值<0.05为条件筛选出所有差异表达miRNA,然后进一步筛选出10个miRNA进行验证,筛选条件为:①2组样本中,至少1组组内平均表达量(TPM)>2;②log2(Fold Change)绝对值≥2;③DKD湿热证组中至少有1个样本TPM>0.最后通过定量RT-PCR在2组受试者中进行验证.结果 将DKD湿热证组及正常对照组的测序数据与数据库miRNA成熟序列进行对比,正常对照组表达的miRNA为302个,DKD湿热证组表达的miRNA为386个,2组共同表达的miRNA为263个.得出97个有差异表达的miRNA,其中上调的84个,下调的13个.DKD湿热证组患者尿液miR-664a-5p、miR-320b及miR-199a-5p表达水平较正常对照组上调(P<0.001).miR-664a-5p、miR-320b及miR-199a-5p在诊断DKD湿热证中的受试者工作特征曲线下面积分别为0.886、0.923及0.761.结论 慢性肾脏病3～4期DKD湿热证患者尿液外泌体miRNA与正常人群比较存在差异表达;尿液外泌体miR-664a-5p、miR-320b和miR-199a-5p有助于DKD湿热证患者的诊断. |
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| AbstractList | R259; 目的 通过外泌体小RNA测序技术,比较慢性肾脏病3～4期糖尿病肾脏疾病(DKD)湿热证患者与正常人群尿液外泌体微RNA(miRNA)的差异表达.方法 2021年4月—2022年4月于安徽中医药大学第一附属医院招募受试者,DKD湿热证患者40例,健康体检正常者40名.2组分别任选10名/例受试者,对尿液外泌体进行miRNA高通量测序,以log2(Fold Change)绝对值≥1及q值<0.05为条件筛选出所有差异表达miRNA,然后进一步筛选出10个miRNA进行验证,筛选条件为:①2组样本中,至少1组组内平均表达量(TPM)>2;②log2(Fold Change)绝对值≥2;③DKD湿热证组中至少有1个样本TPM>0.最后通过定量RT-PCR在2组受试者中进行验证.结果 将DKD湿热证组及正常对照组的测序数据与数据库miRNA成熟序列进行对比,正常对照组表达的miRNA为302个,DKD湿热证组表达的miRNA为386个,2组共同表达的miRNA为263个.得出97个有差异表达的miRNA,其中上调的84个,下调的13个.DKD湿热证组患者尿液miR-664a-5p、miR-320b及miR-199a-5p表达水平较正常对照组上调(P<0.001).miR-664a-5p、miR-320b及miR-199a-5p在诊断DKD湿热证中的受试者工作特征曲线下面积分别为0.886、0.923及0.761.结论 慢性肾脏病3～4期DKD湿热证患者尿液外泌体miRNA与正常人群比较存在差异表达;尿液外泌体miR-664a-5p、miR-320b和miR-199a-5p有助于DKD湿热证患者的诊断. |
| Author | 张磊 王亿平 程梦 张海银 |
| AuthorAffiliation | 安徽中医药大学第一附属医院肾病科 合肥 230031 |
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| Author_FL | WANG Yiping ZHANG Haiyin ZHANG Lei CHENG Meng |
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| Keywords | 尿液 微RNA 糖尿病肾脏疾病 外泌体 湿热证 差异 |
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| Snippet | R259; 目的 通过外泌体小RNA测序技术,比较慢性肾脏病3～4期糖尿病肾脏疾病(DKD)湿热证患者与正常人群尿液外泌体微RNA(miRNA)的差异表达.方法... |
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| Title | 对糖尿病肾脏疾病湿热证患者与正常人群尿液外泌体微RNA差异表达的研究 |
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