沙漠小球藻GTD4C-2胞内酸性糖的提取优化、纯化及抗氧化活性分析
Q71; 本研究通过中心复合设计,采用响应曲面法优化沙漠小球藻GTD4C-2(Chlorella sp.GTD4C-2)胞内多糖(in-tracellular polysaccharide,IPSs)提取工艺.得到最佳提取条件:提取时间:1 h;提取温度:80℃;固液比(g/mL):1:25.通过最佳提取条件,提取的IPSs进行离子色谱分离纯化,而后采用高效凝胶色谱、气相色谱与质谱联用和傅里叶红外技术对IPSs进行结构分析.此外,还对其进行DPPH和羟基自由基清除能力检测.得到分子量稳定的IPS-2和IPS-3两种酸性糖,分子量分别为53.1 kDa和25.6 kDa;硫酸基含量分别为(5.6...
Saved in:
| Published in | 天然产物研究与开发 Vol. 36; no. 7; pp. 1112 - 1120 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
石河子大学生命科学学院,石河子 832003
28.07.2024
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-6880 |
| DOI | 10.16333/j.1001-6880.2024.7.002 |
Cover
| Abstract | Q71; 本研究通过中心复合设计,采用响应曲面法优化沙漠小球藻GTD4C-2(Chlorella sp.GTD4C-2)胞内多糖(in-tracellular polysaccharide,IPSs)提取工艺.得到最佳提取条件:提取时间:1 h;提取温度:80℃;固液比(g/mL):1:25.通过最佳提取条件,提取的IPSs进行离子色谱分离纯化,而后采用高效凝胶色谱、气相色谱与质谱联用和傅里叶红外技术对IPSs进行结构分析.此外,还对其进行DPPH和羟基自由基清除能力检测.得到分子量稳定的IPS-2和IPS-3两种酸性糖,分子量分别为53.1 kDa和25.6 kDa;硫酸基含量分别为(5.69±0.11)％和(8.46±0.03)％;且单糖组成均为葡萄糖、半乳糖.体外抗氧化能力(以vC为阳性对照):VC＞IPSs＞IPS-3＞IPS-2.从沙漠小球藻中分离得到的多糖具有抗氧化能力,且粗提IPSs抗氧化活性强于IPS-2和IPS-3.沙漠小球藻多糖在食品,药品和保健品等方面具有广阔的应用前景. |
|---|---|
| AbstractList | Q71; 本研究通过中心复合设计,采用响应曲面法优化沙漠小球藻GTD4C-2(Chlorella sp.GTD4C-2)胞内多糖(in-tracellular polysaccharide,IPSs)提取工艺.得到最佳提取条件:提取时间:1 h;提取温度:80℃;固液比(g/mL):1:25.通过最佳提取条件,提取的IPSs进行离子色谱分离纯化,而后采用高效凝胶色谱、气相色谱与质谱联用和傅里叶红外技术对IPSs进行结构分析.此外,还对其进行DPPH和羟基自由基清除能力检测.得到分子量稳定的IPS-2和IPS-3两种酸性糖,分子量分别为53.1 kDa和25.6 kDa;硫酸基含量分别为(5.69±0.11)％和(8.46±0.03)％;且单糖组成均为葡萄糖、半乳糖.体外抗氧化能力(以vC为阳性对照):VC＞IPSs＞IPS-3＞IPS-2.从沙漠小球藻中分离得到的多糖具有抗氧化能力,且粗提IPSs抗氧化活性强于IPS-2和IPS-3.沙漠小球藻多糖在食品,药品和保健品等方面具有广阔的应用前景. |
| Abstract_FL | The optimization of intracellular polysaccharides(IPSs)extraction from Chlorella sp.GTD4C-2 was performed u-sing response surface methodology based on a central composite design.The optimal conditions for extraction were determined as follows:an extraction time of 1 h,an extraction temperature of 80 ℃,and a solid-to-liquid ratio of 1:25(g/mL).Subse-quently,the extracted IPSs were further purified through ion exchange chromatography.Structural analysis was carried out u-sing high-performance gel permeation chromatography,gas chromatography-mass spectrometry,and Fourier-transform infrared spectroscopy.Additionally,the DPPH radical scavenging activity and hydroxyl radical scavenging capacity of the IPSs were e-valuated.The results revealed two stable molecular weight acidic polysaccharides:IPS-2(53.1 kDa)and IPS-3(25.6 kDa),both containing glucose and galactose as monosaccharide components.The sulfate content was found to be(5.69±0.11)％for IPS-2 and(8.46±0.03)％for IPS-3.In vitro antioxidant activity showed that vitamin C exhibited the highest antioxidant capacity followed by the crude extract of IPSs,then IPS-3,and finally IPS-2.In conclusion,polysaccharides extracted from Chlorella sp.GTD4C-2 demonstrated significant antioxidant activity with stronger antioxidative properties observed in the crude extract compared to isolated compounds(IPS-2 and IPS-3).These findings suggest promising applications for Chlorella sp.GTD4C-2 polysaccharides in various fields such as food products,pharmaceuticals,and health supplements. |
| Author | 周永顺 高剑峰 陈福龙 王璐 韩凯 张凯月 |
| AuthorAffiliation | 石河子大学生命科学学院,石河子 832003 |
| AuthorAffiliation_xml | – name: 石河子大学生命科学学院,石河子 832003 |
| Author_FL | WANG Lu ZHANG Kai-yue CHEN Fu-long GAO Jian-feng HAN Kai ZHOU Yong-shun |
| Author_FL_xml | – sequence: 1 fullname: ZHANG Kai-yue – sequence: 2 fullname: ZHOU Yong-shun – sequence: 3 fullname: HAN Kai – sequence: 4 fullname: WANG Lu – sequence: 5 fullname: CHEN Fu-long – sequence: 6 fullname: GAO Jian-feng |
| Author_xml | – sequence: 1 fullname: 张凯月 – sequence: 2 fullname: 周永顺 – sequence: 3 fullname: 韩凯 – sequence: 4 fullname: 王璐 – sequence: 5 fullname: 陈福龙 – sequence: 6 fullname: 高剑峰 |
| BookMark | eNo9kEFLAkEcxedgkJmfoXuw23_-s-6Ox7CyQOiiZ5lxdyKLFdxCvBmmhATraa0O0s1jUh0i_Ta1s_Yt2ig6vR-Px3vwNkjGb_keIVsUTGozxnaaJgWghs05mAhomY4JgBmS_bfXST4ITiUAcBsReZbU9PO9Xj7G8zAZ91eTRbm6Z5UMXPWn8XDwNXjTvVnyEiUP1zocx2H0ubyLb6OP3lXy_pRCHI70aKLns5T16yINxzdDPR1vkjUlzgMv_6c5UjvYr5YOjcpx-ai0WzECCgU0GEjbahSLKBQI6iIqwVxlNbhCRpV0hcMRlMe5yxzFpMtcBywmpScBC1JxliPbv70d4Svhn9Sbrcu2ny7WL9qNTrfZPVM_R4CT_sC-AWb5cQM |
| ClassificationCodes | Q71 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.16333/j.1001-6880.2024.7.002 |
| DatabaseName | Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry |
| DocumentTitle_FL | Extraction optimization and purification of intracellular acidic polysaccharides from Chlorella sp.GTD4C-2 and their antioxidant activity |
| EndPage | 1120 |
| ExternalDocumentID | trcwyjykf202407002 |
| GrantInformation_xml | – fundername: (国家自然科学基金); (国家自然科学基金) funderid: (国家自然科学基金); (国家自然科学基金) |
| GroupedDBID | -01 2B. 4A8 5XA 5XC 8VB 92E 92I 93N ABJNI ABOCM ACGFS AHQJS AKVCP ALMA_UNASSIGNED_HOLDINGS CCEZO CCVFK CW9 EBU EOJEC GROUPED_DOAJ K1G OBODZ PSX QWB TCJ TGP TH9 TUS U1G U5L ZL0 ~8M |
| ID | FETCH-LOGICAL-s1052-30b64c992af0a1d22fa3df4c8f231fbda7820fe88d37f3bd3d7043bbeb025bf83 |
| ISSN | 1001-6880 |
| IngestDate | Thu May 29 04:03:03 EDT 2025 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 7 |
| Keywords | 工艺优化 沙漠小球藻 Chlorella sp 多糖 process optimization 响应曲面 intracellular polysaccharides 抗氧化活性 response surface antioxidant activity |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-s1052-30b64c992af0a1d22fa3df4c8f231fbda7820fe88d37f3bd3d7043bbeb025bf83 |
| PageCount | 9 |
| ParticipantIDs | wanfang_journals_trcwyjykf202407002 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-07-28 |
| PublicationDateYYYYMMDD | 2024-07-28 |
| PublicationDate_xml | – month: 07 year: 2024 text: 2024-07-28 day: 28 |
| PublicationDecade | 2020 |
| PublicationTitle | 天然产物研究与开发 |
| PublicationTitle_FL | Natural Product Research and Development |
| PublicationYear | 2024 |
| Publisher | 石河子大学生命科学学院,石河子 832003 |
| Publisher_xml | – name: 石河子大学生命科学学院,石河子 832003 |
| SSID | ssib000862228 ssj0039521 ssib051371382 ssib053273375 |
| Score | 2.3616984 |
| Snippet | Q71; 本研究通过中心复合设计,采用响应曲面法优化沙漠小球藻GTD4C-2(Chlorella sp.GTD4C-2)胞内多糖(in-tracellular polysaccharide,IPSs)提取工艺.得到最佳提取条件:提取时间:1... |
| SourceID | wanfang |
| SourceType | Aggregation Database |
| StartPage | 1112 |
| Title | 沙漠小球藻GTD4C-2胞内酸性糖的提取优化、纯化及抗氧化活性分析 |
| URI | https://d.wanfangdata.com.cn/periodical/trcwyjykf202407002 |
| Volume | 36 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnR1Na9VAMNR60Iv4id8UdE-Smuzuy-4ek9fEIuiphd5K8pIoCk9oX5H2VKktUoTXU6seirceLepBbH-LF3159V84s9nkBSyiXsJkZnZ2PrKZ3WU_LOt2mkg3c_OWTfEiQJ5mni3jLLcZlx0uVJJLF_cOP3joTc_y-3OtubET3xqrlpZ6yWRn5dh9Jf8TVcBBXHGX7D9EthYKCIAhvvCECMPzr2JMQo8ElCilgTbxHRK2SOAQGZFQEAUAI6EkSpAguDczxds2xXfAqhBZpUdki4QKn4FEKdIhvsDCAfB4WopPJNekCCViqUiTOFappMa0NYZhcenq4j7xowZJl5K-luOjQqhxWVfNAxiQGTTUAJJEJQEDCpc3flZdaaT6nPgKqwMNg1Il3-gvlSGBUxTXgKp4QKY2H_SXtUX1t2copTOl0HZA_cArmyzKJb40ZqDvoD6XjKY9EKEirYORMqIIrF8G2ru0MstMvVCOc7pmKzs2Fs0VYTzKaCMAlk-ZYKALhMH4ZcA48pcaBlPacoHwiKcGFH47PoV29oda7sDv2HFYI33hAjlPlndjVfmtPGDGtGPRSFaQ5mij4wNvzrFJ1WM4649ZtRI_id6YxMM_6agfUa_u7C10ni8_WX6aIxekFDzr9SQVolxGYaY8quE0HQ1_Wy4TeCRm_c6gb81Gh2gz1Sr3UVZqmPWaqN_d47XTW_G6edx91Og1zpy1zpjh3oRftt1z1tjK4_PWqXZ1y-IFa7b4-LY4fD_Y7w-31o52DkwrPVrbHWys_1z_UqzuDT9tD9-9LPpbg_72j8M3g9fb31dfDL9-AGDQ3yw2d4r9PYCLzwfAPHi1UexuXbRmo3CmPW2bq07sRRjgUJs5icc7StE4d2I3pTSPWZrzjsxh_JUnaYzHWuaZlCkTOUtSlgqHsyTJEhizwA-VXbLGu8-62WVrwvOy2I2zWPAk5TC-kSzvCJFm1EmSPHOdK9Yt45J58ytbnP89Ylf_iuuadXrULq5b472FpewGdNJ7yU0d6V806bF- |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E6%B2%99%E6%BC%A0%E5%B0%8F%E7%90%83%E8%97%BBGTD4C-2%E8%83%9E%E5%86%85%E9%85%B8%E6%80%A7%E7%B3%96%E7%9A%84%E6%8F%90%E5%8F%96%E4%BC%98%E5%8C%96%E3%80%81%E7%BA%AF%E5%8C%96%E5%8F%8A%E6%8A%97%E6%B0%A7%E5%8C%96%E6%B4%BB%E6%80%A7%E5%88%86%E6%9E%90&rft.jtitle=%E5%A4%A9%E7%84%B6%E4%BA%A7%E7%89%A9%E7%A0%94%E7%A9%B6%E4%B8%8E%E5%BC%80%E5%8F%91&rft.au=%E5%BC%A0%E5%87%AF%E6%9C%88&rft.au=%E5%91%A8%E6%B0%B8%E9%A1%BA&rft.au=%E9%9F%A9%E5%87%AF&rft.au=%E7%8E%8B%E7%92%90&rft.date=2024-07-28&rft.pub=%E7%9F%B3%E6%B2%B3%E5%AD%90%E5%A4%A7%E5%AD%A6%E7%94%9F%E5%91%BD%E7%A7%91%E5%AD%A6%E5%AD%A6%E9%99%A2%2C%E7%9F%B3%E6%B2%B3%E5%AD%90+832003&rft.issn=1001-6880&rft.volume=36&rft.issue=7&rft.spage=1112&rft.epage=1120&rft_id=info:doi/10.16333%2Fj.1001-6880.2024.7.002&rft.externalDocID=trcwyjykf202407002 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Ftrcwyjykf%2Ftrcwyjykf.jpg |