靶向鸡糖皮质激素受体基因的microRNA预测与鉴定
S831.1; [目的]本文旨在预测和筛选靶向调控鸡糖皮质激素受体(glucocorticoid receptor,GR)表达的microRNA.[方法]用TargetScan、PicTar和miRDB软件分别预测靶向鸡GR 3′UTR microRNA并取交集;构建包含鸡GR 3′UTR的重组质粒及突变质粒,通过双荧光素酶试验鉴定microRNA和鸡GR 3′UTR的靶向性;用TargetScan和miRDB软件分别预测候选microRNA的靶基因,用DAVID软件对预测出的共同靶基因进行GO功能分析;用Western blot检测过表达候选microRNA对鸡DF1 细胞 GR蛋白表达的影...
Saved in:
| Published in | 南京农业大学学报 Vol. 47; no. 3; pp. 489 - 496 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095
2024
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-2030 |
| DOI | 10.7685/jnau.202305021 |
Cover
Loading…
| Abstract | S831.1; [目的]本文旨在预测和筛选靶向调控鸡糖皮质激素受体(glucocorticoid receptor,GR)表达的microRNA.[方法]用TargetScan、PicTar和miRDB软件分别预测靶向鸡GR 3′UTR microRNA并取交集;构建包含鸡GR 3′UTR的重组质粒及突变质粒,通过双荧光素酶试验鉴定microRNA和鸡GR 3′UTR的靶向性;用TargetScan和miRDB软件分别预测候选microRNA的靶基因,用DAVID软件对预测出的共同靶基因进行GO功能分析;用Western blot检测过表达候选microRNA对鸡DF1 细胞 GR蛋白表达的影响.[结果]miR124-3p、miR142-3p、miR204/211、miR183、miR18-5p和miR181a/181b是 3 种软件预测靶向GR 3′UTR的交集microRNA;双荧光素酶试验结果表明miR142-3p、miR204/211 和 miR18-5p靶向结合 GR 3′UTR.2 种软件预测 3 个候选microRNA的靶基因中都有GR且富集分子功能不同;在DF1 细胞中过表达 3 个候选microRNA后,miR142-3p和miR204/211 显著降低GR蛋白的表达水平(P<0.05).[结论]miR142-3p、miR204/211 和miR18-5p靶向结合鸡GR 3′UTR,且miR142-3p和miR204/21 可以降低鸡DF1 细胞中GR蛋白的表达水平. |
|---|---|
| AbstractList | S831.1; [目的]本文旨在预测和筛选靶向调控鸡糖皮质激素受体(glucocorticoid receptor,GR)表达的microRNA.[方法]用TargetScan、PicTar和miRDB软件分别预测靶向鸡GR 3′UTR microRNA并取交集;构建包含鸡GR 3′UTR的重组质粒及突变质粒,通过双荧光素酶试验鉴定microRNA和鸡GR 3′UTR的靶向性;用TargetScan和miRDB软件分别预测候选microRNA的靶基因,用DAVID软件对预测出的共同靶基因进行GO功能分析;用Western blot检测过表达候选microRNA对鸡DF1 细胞 GR蛋白表达的影响.[结果]miR124-3p、miR142-3p、miR204/211、miR183、miR18-5p和miR181a/181b是 3 种软件预测靶向GR 3′UTR的交集microRNA;双荧光素酶试验结果表明miR142-3p、miR204/211 和 miR18-5p靶向结合 GR 3′UTR.2 种软件预测 3 个候选microRNA的靶基因中都有GR且富集分子功能不同;在DF1 细胞中过表达 3 个候选microRNA后,miR142-3p和miR204/211 显著降低GR蛋白的表达水平(P<0.05).[结论]miR142-3p、miR204/211 和miR18-5p靶向结合鸡GR 3′UTR,且miR142-3p和miR204/21 可以降低鸡DF1 细胞中GR蛋白的表达水平. |
| Abstract_FL | [Objectives]This study aimed to predict and screen microRNA that targeted the expression of chicken glucocorticoid receptor(GR).[Methods]The microRNA targeting chicken GR 3′UTR was predicted by TargetScan,PicTar and miRDB software,respectively,and the overlapping microRNA was selected as candidates for further experiment.The recombinant plasmid and mutant plasmid containing chicken GR 3′UTR were constructed,and microRNA binding to chicken GR 3′UTR was verified by double luciferase assay.The target genes of candidate microRNA were predicted by TargetScan and miRDB software,respectively,and the GO function of the predicted common target genes was analyzed by DAVID software.Western blot was used to detect the expression of GR protein after overexpression of candidate microRNA in chicken DF1 cells.[Results]miR124-3p,miR142-3p,miR204/211,miR183,miR18-5p,and miR181a/181b were the intersection microRNA of 3 softwares prediction targeting GR 3′UTR.Dual luciferase assay showed that miR142-3p,miR204/211 and miR18-5p targeted GR 3′UTR.The two kinds of software predicted that the target genes of three candidate microRNA contained GR and the enriched molecular functions were different.After overexpression of the three candidate microRNA in DF1 cell,miR142-3p and miR204/211 significantly decreased the expression of GR protein level(P<0.05).[Conclusions]miR142-3p,miR204/211 and miR18-5p target chicken GR 3′UTR,and miR142-3p and miR204/21 can reduce the expression level of GR in chicken DF1 cell. |
| Author | 刘杰 赵敏蝶 赵茹茜 |
| AuthorAffiliation | 南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095 |
| AuthorAffiliation_xml | – name: 南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095 |
| Author_FL | ZHAO Mindie LIU Jie ZHAO Ruqian |
| Author_FL_xml | – sequence: 1 fullname: ZHAO Mindie – sequence: 2 fullname: LIU Jie – sequence: 3 fullname: ZHAO Ruqian |
| Author_xml | – sequence: 1 fullname: 赵敏蝶 – sequence: 2 fullname: 刘杰 – sequence: 3 fullname: 赵茹茜 |
| BookMark | eNrjYmDJy89LZWAQMzTQMzezMNXPykss1TMyMDI2MDUwMmRh4DQ0MDDQNTIwNuBg4C0uzkwyMDU0NgdKWnAy2L2cu-3phIkvdyx8vnna81nrXmxZ8Wx_w_MtC572T3-yd_LT-buezl7wfFZLbmZyUX6Qn-PLRS3PtnY_2dH3snPL03WzeBhY0xJzilN5oTQ3Q6iba4izh66Pv7uns6OPbrGhgYmlrlGSQZKJgaEF0A3GloZGpoYWxkBWikVisolpiomFsVFaaqJxqoGhSUpqYlqyUVKqSZKFkUWagblhkmVKqmWKMTeDJsTc8sS8tMS89Pis_NKiPKCN8XlZeZUpFRVJQP-aAH1oYGkMAMXCX-4 |
| ClassificationCodes | S831.1 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.7685/jnau.202305021 |
| DatabaseName | Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Agriculture |
| DocumentTitle_FL | Prediction and identification of microRNA targeting chicken glucocorticoid receptor gene |
| EndPage | 496 |
| ExternalDocumentID | njnydxxb202403009 |
| GrantInformation_xml | – fundername: (国家自然科学基金); (国家自然科学基金); (国家重点研发计划) funderid: (国家自然科学基金); (国家自然科学基金); (国家重点研发计划) |
| GroupedDBID | -04 123 2B. 4A8 92G 92I 93N ABDBF ABJNI ACGFS ACUHS ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CW9 DYU EOJEC ESX OBODZ OZF PSX TCJ TGD U1G U5N UY8 |
| ID | FETCH-LOGICAL-s1049-2b0b401820339125183033d8ac45d4832fea3e014deafc2be4b828f071b9de9d3 |
| ISSN | 1000-2030 |
| IngestDate | Thu May 29 04:08:26 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Keywords | 鸡 靶向基因 chicken glucocorticoid receptor 糖皮质激素受体 microRNA targeting gene |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-s1049-2b0b401820339125183033d8ac45d4832fea3e014deafc2be4b828f071b9de9d3 |
| PageCount | 8 |
| ParticipantIDs | wanfang_journals_njnydxxb202403009 |
| PublicationCentury | 2000 |
| PublicationDate | 2024 |
| PublicationDateYYYYMMDD | 2024-01-01 |
| PublicationDate_xml | – year: 2024 text: 2024 |
| PublicationDecade | 2020 |
| PublicationTitle | 南京农业大学学报 |
| PublicationTitle_FL | Journal of Nanjing Agricultural University |
| PublicationYear | 2024 |
| Publisher | 南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095 |
| Publisher_xml | – name: 南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095 |
| SSID | ssib051370508 ssj0041892 ssib001101132 ssib002263529 |
| Score | 2.3402903 |
| Snippet | S831.1; [目的]本文旨在预测和筛选靶向调控鸡糖皮质激素受体(glucocorticoid receptor,GR)表达的microRNA.[方法]用TargetScan、PicTar和miRDB软件分别预测靶向鸡GR 3′UTR microRNA并取交集;构建包含鸡GR... |
| SourceID | wanfang |
| SourceType | Aggregation Database |
| StartPage | 489 |
| Title | 靶向鸡糖皮质激素受体基因的microRNA预测与鉴定 |
| URI | https://d.wanfangdata.com.cn/periodical/njnydxxb202403009 |
| Volume | 47 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LaxRBEB5ivOhBfOKbIPYpbJzp7pnpvgg9u7MEwRwkgXgKO69IwBHygJiTh1xE8IGgEBGjJ0-5GCUI4p_JZt1foVU1neyY5BCFZShquuvR38521Wx3tePc5IWQgcQ37nnhNmQmONaATBspJGI64GkaUomNuxPB-JS8M-1PDx37XVu1tLSYjKUrh-4r-R9UgQe44i7Zf0B2TygwgAZ84QoIw_VIGLNYM91iUcBin2mXaQ85kWIGiJBFgukACW2YiVmsWCSZUSwOWNTGJQ7YBjgudldtpkMWSxa1mBYksM0iQ0REbUiOkg9xCd-9CYOqDAcGyQMBEfVWTMV4S2mUDb1Bszb1GJi0tXa1gWlN4gRMN60ETWoNmBYS0WImqBEBU9DL3_2ukGM-fuCOJk-AQ-MyaAJs4JHveMc9pDNY3mSRtoRu1l-I8MGr0P83H61WOI4wNkYTBxRGtTYAJw0qbx8u03LIhwpsJUjmQeGAlkQIkXCx44BDejWpUyH6gr04uV7BRQAag80qDvjCSW_kkQSFaKv26MGxGIXk3q0ONLVzHRYV4K79W8xOhlX5U_vQi9rMJquTnmyQJKtjiPfPv5C7YqmSubKzNMYxu_Xdav_7vprm5Vz5OFteThA-MAA34B7nkOfhzNq6PzWI52HC8Or19bB00iCe9z0Rgoq9vw2lp3S1vMF6VlVpRatu_W0TbdEri045W4smJ087p2waOGKqZ_qMM7Ty4Kxz0szO21I4-Tnndv_9t-7LV_2tj70vb3prG782P-_8fNLbXO--eLv943X3w_fuu_Xe2uru89j_tLrz9dn21vP-083uxtp5Z6odTzbHG_a0k8aCB2l6gyduIl08T0EIjWmHguhSZKqTSj-TMPEWeUfkriezvFOkPMllorgqIEVIdJbrTFxwhstHZX7RGdG5j4tcApFA9M5T2cGqoVlYJHmYykQEl5wb1vsZ-2u2MHMAkstHaXTFOYF09U7yqjO8OL-UX4MofTG5Tkj-AbCTp5A |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E9%9D%B6%E5%90%91%E9%B8%A1%E7%B3%96%E7%9A%AE%E8%B4%A8%E6%BF%80%E7%B4%A0%E5%8F%97%E4%BD%93%E5%9F%BA%E5%9B%A0%E7%9A%84microRNA%E9%A2%84%E6%B5%8B%E4%B8%8E%E9%89%B4%E5%AE%9A&rft.jtitle=%E5%8D%97%E4%BA%AC%E5%86%9C%E4%B8%9A%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5&rft.au=%E8%B5%B5%E6%95%8F%E8%9D%B6&rft.au=%E5%88%98%E6%9D%B0&rft.au=%E8%B5%B5%E8%8C%B9%E8%8C%9C&rft.date=2024&rft.pub=%E5%8D%97%E4%BA%AC%E5%86%9C%E4%B8%9A%E5%A4%A7%E5%AD%A6%E5%8A%A8%E7%89%A9%E5%8C%BB%E5%AD%A6%E9%99%A2%2F%E5%86%9C%E4%B8%9A%E5%86%9C%E6%9D%91%E9%83%A8%E5%8A%A8%E7%89%A9%E7%94%9F%E7%90%86%E7%94%9F%E5%8C%96%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E6%B1%9F%E8%8B%8F+%E5%8D%97%E4%BA%AC+210095&rft.issn=1000-2030&rft.volume=47&rft.issue=3&rft.spage=489&rft.epage=496&rft_id=info:doi/10.7685%2Fjnau.202305021&rft.externalDocID=njnydxxb202403009 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fnjnydxxb%2Fnjnydxxb.jpg |