三七悬浮细胞生物合成绿原酸类物质及其生物活性

TS201.3; 利用三七(Panax notoginseng)悬浮细胞生物合成绿原酸类物质(CGAs),并对其生物活性进行了研究.对三七细胞中CGAs进行定性分析,优化了细胞培养条件以及 6种诱导模式,测定了三七细胞CGAs的抗氧化及α-葡萄糖苷酶活性抑制能力.结果表明三七愈伤组织内含有4种CGAs;最优工艺条件为:B5+40 g/L蔗糖,pH 7.5,15%接种量;经30 μmol/L 茉莉酸甲酯诱导3d后,CGAs产量(684.07 mg/L)比原工艺(475.18 mg/L)提高了0.44倍;三七细胞CGAs具有良好的抗氧化及α-葡萄糖苷酶活性抑制能力....

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Published in华东理工大学学报（自然科学版） Vol. 49; no. 4; pp. 515 - 528
Main Authors 周倍, 刘楠, 刘泽波, 庄英萍, 王泽建, 郭美锦
Format Journal Article
LanguageChinese
Published 华东理工大学生物反应器工程国家重点实验室, 上海 200237 01.08.2023
Subjects
Panax notoginseng calli
工艺优化
悬浮培养
bioactivity
绿原酸生物合成
chlorogenic acid biosynthesis
三七愈伤组织细胞
process optimization
生物活性
suspension culture
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Abstract TS201.3; 利用三七(Panax notoginseng)悬浮细胞生物合成绿原酸类物质(CGAs),并对其生物活性进行了研究.对三七细胞中CGAs进行定性分析,优化了细胞培养条件以及 6种诱导模式,测定了三七细胞CGAs的抗氧化及α-葡萄糖苷酶活性抑制能力.结果表明三七愈伤组织内含有4种CGAs;最优工艺条件为:B5+40 g/L蔗糖,pH 7.5,15%接种量;经30 μmol/L 茉莉酸甲酯诱导3d后,CGAs产量(684.07 mg/L)比原工艺(475.18 mg/L)提高了0.44倍;三七细胞CGAs具有良好的抗氧化及α-葡萄糖苷酶活性抑制能力.
AbstractList TS201.3; 利用三七(Panax notoginseng)悬浮细胞生物合成绿原酸类物质(CGAs),并对其生物活性进行了研究.对三七细胞中CGAs进行定性分析,优化了细胞培养条件以及 6种诱导模式,测定了三七细胞CGAs的抗氧化及α-葡萄糖苷酶活性抑制能力.结果表明三七愈伤组织内含有4种CGAs;最优工艺条件为:B5+40 g/L蔗糖,pH 7.5,15%接种量;经30 μmol/L 茉莉酸甲酯诱导3d后,CGAs产量(684.07 mg/L)比原工艺(475.18 mg/L)提高了0.44倍;三七细胞CGAs具有良好的抗氧化及α-葡萄糖苷酶活性抑制能力.
Author 刘楠
庄英萍
刘泽波
郭美锦
王泽建
周倍
AuthorAffiliation 华东理工大学生物反应器工程国家重点实验室, 上海 200237
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Author_FL LIU Zebo
LIU Nan
ZHOU Bei
WANG Zejian
GUO Meijin
ZHUANG Yingping
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  fullname: 周倍
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DocumentTitle_FL Biosynthesis and Bioactivity of Chlorogenic Acids in Panax notoginseng Suspension Cells
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Keywords Panax notoginseng calli
工艺优化
悬浮培养
bioactivity
绿原酸生物合成
chlorogenic acid biosynthesis
三七愈伤组织细胞
process optimization
生物活性
suspension culture
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Snippet TS201.3; 利用三七(Panax notoginseng)悬浮细胞生物合成绿原酸类物质(CGAs),并对其生物活性进行了研究.对三七细胞中CGAs进行定性分析,优化了细胞培养条件以及...
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StartPage 515
Title 三七悬浮细胞生物合成绿原酸类物质及其生物活性
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