等离子体作用结合氧限制模型选育辅酶Q10高产菌株

TS201.3; 将常温常压等离子体(Atmospheric and Room Temperature Plasma,ARTP)诱变和无水亚硫酸钠构建的氧限制筛选模型相结合,通过适合辅酶Q10生产菌株的24孔板高通量快速培养技术,选育了类球红细菌Rhodobacter sphaeroides耐氧限的高性能突变株.实验结果表明:该菌株诱变筛选最合适的ARTP诱变时间为25 s,氧限制平板上无水亚硫酸钠的最佳质量浓度为0.4 g/L,24孔板上该菌氧传递的最优装液量为2.0 mL、发酵周期为48 h,在上述条件下,最终选育出了氧限制条件下辅酶Q10合成效率高的突变菌株R.sphaeroides F...

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Published in华东理工大学学报（自然科学版） Vol. 47; no. 3; pp. 308 - 315
Main Authors 张幸子, 王晓惠, 王泽建, 陈必钦, 李丹, 郭美锦, 储炬, 庄英萍
Format Journal Article
LanguageChinese
Published 华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心(上海),上海 200237%内蒙古金达威药业有限公司,呼和浩特 010000 30.06.2021
Subjects
高通量筛选
氧限制模型
辅酶Q10
常温常压等离子体
类球红细菌
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Abstract TS201.3; 将常温常压等离子体(Atmospheric and Room Temperature Plasma,ARTP)诱变和无水亚硫酸钠构建的氧限制筛选模型相结合,通过适合辅酶Q10生产菌株的24孔板高通量快速培养技术,选育了类球红细菌Rhodobacter sphaeroides耐氧限的高性能突变株.实验结果表明:该菌株诱变筛选最合适的ARTP诱变时间为25 s,氧限制平板上无水亚硫酸钠的最佳质量浓度为0.4 g/L,24孔板上该菌氧传递的最优装液量为2.0 mL、发酵周期为48 h,在上述条件下,最终选育出了氧限制条件下辅酶Q10合成效率高的突变菌株R.sphaeroides F5D13.在5 L发酵罐分批培养过程中,该高产菌株表现出了较强的氧亲和力,单位菌体的合成效率比出发菌株提升了18％.
AbstractList TS201.3; 将常温常压等离子体(Atmospheric and Room Temperature Plasma,ARTP)诱变和无水亚硫酸钠构建的氧限制筛选模型相结合,通过适合辅酶Q10生产菌株的24孔板高通量快速培养技术,选育了类球红细菌Rhodobacter sphaeroides耐氧限的高性能突变株.实验结果表明:该菌株诱变筛选最合适的ARTP诱变时间为25 s,氧限制平板上无水亚硫酸钠的最佳质量浓度为0.4 g/L,24孔板上该菌氧传递的最优装液量为2.0 mL、发酵周期为48 h,在上述条件下,最终选育出了氧限制条件下辅酶Q10合成效率高的突变菌株R.sphaeroides F5D13.在5 L发酵罐分批培养过程中,该高产菌株表现出了较强的氧亲和力,单位菌体的合成效率比出发菌株提升了18％.
Author 庄英萍
郭美锦
王晓惠
王泽建
李丹
张幸子
陈必钦
储炬
AuthorAffiliation 华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心(上海),上海 200237%内蒙古金达威药业有限公司,呼和浩特 010000
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CHU Ju
LI Dan
ZHANG Xingzi
WANG Zejian
GUO Meijin
WANG Xiaohui
ZHUANG Yingping
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DocumentTitle_FL Screening of High-Yield Coenzyme Q10 Producing Strain by Using Atmospheric and Room Temperature Plasma and Oxygen-Limited Model
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Keywords 高通量筛选
氧限制模型
辅酶Q10
常温常压等离子体
类球红细菌
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Snippet TS201.3; 将常温常压等离子体(Atmospheric and Room Temperature Plasma,ARTP)诱变和无水亚硫酸钠构建的氧限制筛选模型相结合,通过适合辅酶Q10生产菌株的24孔板高通量快速培养技术,选育了类球红细菌Rhodobacter...
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StartPage 308
Title 等离子体作用结合氧限制模型选育辅酶Q10高产菌株
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