不同强度运动改善PTSD小鼠恐惧记忆泛化及促海马神经再生效应研究

R395.4%R455%R749; 目的:探讨不同强度运动缓解创伤后应激障碍(PTSD)恐惧记忆泛化的作用及促海马神经再生中枢调控机制.方法:采用随机数字法将雄性C57 BL/6J小鼠分为对照(control)组、PTSD建模(PTSD)组、建模后高强度运动(PTSD-High)组和建模后低强度运动(PTSD-Low)组.采用条件性足部电击(CF)和单次-持续应激(SPS)相结合的方法,构建PTSD复合应激模型.利用条件性恐惧实验测试小鼠对恐惧记忆相似情境的辨别能力,评估小鼠恐惧记忆泛化程度,通过免疫荧光双标实验观察并量化小鼠海马DG区新生的未成熟神经元,使用ELISA测定血清脂联素的分泌水平...

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Published in中国病理生理杂志 Vol. 40; no. 6; pp. 1017 - 1024
Main Authors 金硕, 张晓晓, 吉宸萱, 孙丽娜
Format Journal Article
LanguageChinese
Published 北京师范大学体育与运动学院,北京 100875%北京师范大学体育与运动学院,北京 100875 2024
北京师范大学认知神经科学与学习国家重点实验室,北京 100875
Subjects
exercise intensity
adiponectin
post-traumatic stress disorder
恐惧记忆泛化
adult hippocampal neurogenesis
运动强度
fear memory generalization
创伤后应激障碍
脂联素
海马神经再生
Online AccessGet full text
ISSN1000-4718
DOI10.3969/j.issn.1000-4718.2024.06.007

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Abstract R395.4%R455%R749; 目的:探讨不同强度运动缓解创伤后应激障碍(PTSD)恐惧记忆泛化的作用及促海马神经再生中枢调控机制.方法:采用随机数字法将雄性C57 BL/6J小鼠分为对照(control)组、PTSD建模(PTSD)组、建模后高强度运动(PTSD-High)组和建模后低强度运动(PTSD-Low)组.采用条件性足部电击(CF)和单次-持续应激(SPS)相结合的方法,构建PTSD复合应激模型.利用条件性恐惧实验测试小鼠对恐惧记忆相似情境的辨别能力,评估小鼠恐惧记忆泛化程度,通过免疫荧光双标实验观察并量化小鼠海马DG区新生的未成熟神经元,使用ELISA测定血清脂联素的分泌水平.结果:(1)在条件性恐惧实验三个类似情境中,control组与PTSD-High组的不动时间均明显低于PTSD组.(2)免疫荧光双标染色图片显示,PTSD组小鼠海马DG区的新生神经元与未成熟神经元荧光信号降低,新生未成熟神经元纤维短而少.统计表明,与PTSD组相比,control、PTSD-High和PTSD-Low组小鼠新生未成熟神经元的细胞密度和树突分支点及长度均明显升高.(3)ELISA结果显示,control和PTSD-High组小鼠血清中脂联素水平均明显高于PTSD组和PTSD-Low组.结论:PTSD小鼠恐惧记忆泛化与海马神经再生水平下降有关.运动可能通过促进脂联素的分泌、促进海马神经再生改善恐惧记忆泛化.不同强度运动对比发现高强度运动改善效果更好.
AbstractList R395.4%R455%R749; 目的:探讨不同强度运动缓解创伤后应激障碍(PTSD)恐惧记忆泛化的作用及促海马神经再生中枢调控机制.方法:采用随机数字法将雄性C57 BL/6J小鼠分为对照(control)组、PTSD建模(PTSD)组、建模后高强度运动(PTSD-High)组和建模后低强度运动(PTSD-Low)组.采用条件性足部电击(CF)和单次-持续应激(SPS)相结合的方法,构建PTSD复合应激模型.利用条件性恐惧实验测试小鼠对恐惧记忆相似情境的辨别能力,评估小鼠恐惧记忆泛化程度,通过免疫荧光双标实验观察并量化小鼠海马DG区新生的未成熟神经元,使用ELISA测定血清脂联素的分泌水平.结果:(1)在条件性恐惧实验三个类似情境中,control组与PTSD-High组的不动时间均明显低于PTSD组.(2)免疫荧光双标染色图片显示,PTSD组小鼠海马DG区的新生神经元与未成熟神经元荧光信号降低,新生未成熟神经元纤维短而少.统计表明,与PTSD组相比,control、PTSD-High和PTSD-Low组小鼠新生未成熟神经元的细胞密度和树突分支点及长度均明显升高.(3)ELISA结果显示,control和PTSD-High组小鼠血清中脂联素水平均明显高于PTSD组和PTSD-Low组.结论:PTSD小鼠恐惧记忆泛化与海马神经再生水平下降有关.运动可能通过促进脂联素的分泌、促进海马神经再生改善恐惧记忆泛化.不同强度运动对比发现高强度运动改善效果更好.
Abstract_FL AIM:To explore the intensity-dependent effects of exercises to alleviate fear memory generaliza-tion in post-traumatic stress disorder(PTSD)and the underlying mechanism.METHODS:Male C57 BL/6J mice were randomly divided into the control group,PTSD group,high-intensity exercise(PTSD-High)group,and low-intensity exer-cise(PTSD-Low)group.PTSD model were created via using a combination of conditioned foot shock(CF)and single-pro-longed stress(SPS).The contextual fear test was used to test the mice's ability to discriminate safety situations from fear condition.Immunofluorescence observed and quantified the newborn immature neurons in the DG area of the mice's hippo-campus.ELISA was used to determine the secretion level of serum tissue adiponectin.RESULTS:(1)The immobility times for the PTSD-High and Control groups were significantly lower than those of the PTSD group.(2)Immunofluores-cence analysis showed that the cell density,dendritic branching points and length of newborn immature neurons were ele-vated in High and Low groups compared to the PTSD group.(3)Adiponectin levels in the serum of the control and PTSD-High group were significantly higher than those in the PTSD group and PTSD-Low group.CONCLUSION:Fear memory establishment in PTSD mice is associated with decreased hippocampal neurogenesis.High-intensity exercise ameliorated fear memory by enhancing adiponectin secretion and promoting hippocampal neurogenesis.High-intensity exercise exerted better improvement of brain functions to PTSD model.
Author 孙丽娜
张晓晓
吉宸萱
金硕
AuthorAffiliation 北京师范大学体育与运动学院,北京 100875%北京师范大学体育与运动学院,北京 100875;北京师范大学认知神经科学与学习国家重点实验室,北京 100875
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Author_FL SUN Lina
JI Chenxuan
JIN Shuo
ZHANG Xiaoxiao
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DocumentTitle_FL Different exercise intensity alleviates fear memory generalization in PTSD mice by promoting adiponectin mediated adult hippocampal neurogene-sis
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Keywords exercise intensity
adiponectin
post-traumatic stress disorder
恐惧记忆泛化
adult hippocampal neurogenesis
运动强度
fear memory generalization
创伤后应激障碍
脂联素
海马神经再生
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Title 不同强度运动改善PTSD小鼠恐惧记忆泛化及促海马神经再生效应研究
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