Small Amplicons Mutation Library for Vaccine Screening by Error-Prone Polymerase Chain Reaction

• Published in: 东华大学学报（英文版） Vol. 40; no. 2; pp. 171 - 176
• Main Authors: CHENG Manman, ZHANG Yunlong, CHEN Ting, ZHANG Minmin, LU Changrui
• Format: Journal Article
• Language: English
• Published: College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China 01.04.2023
• Subjects: error-prone polymerase chain reaction; in vitro transcription; DNA library; RNA library

Q523.8; Library construction is a common method used to screen target genes in molecular biology.Most library constructions are not suitable for a small DNA library(＜100 base pair(bp))and low RNA library output.To maximize the library's complexity,error-prone polymerase chain reaction(PCR)was u...