放置时间对大鼠血清样本中电解质含量及pH值的影响
R-332%Q95-33; 目的 观察SD大鼠血清样本放置时间对血清中K+、Na+、Ca2+、Cl-含量和pH值检测结果的影响.方法 取雌雄SD大鼠各20只,腹腔静脉采血后分离血清,样本加盖存放于2～8℃,在0h、2h、4h、6h、24h、48 h和120 h共7个时间点分别测定K+、Na+、Ca2+、Cl-含量和pH值.结果 K+、Na+含量各时间点比较差异无统计学意义(P>0.05);与0 h检测结果比较,放置48 h和120 h的Cl-含量明显升高(P<0.05),放置48 h和120 h的Ca 2+含量明显降低(P<0.05),放置24 h、48 h、120 h的pH...
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| Published in | 实验动物与比较医学 Vol. 42; no. 4; pp. 301 - 305 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
海南省疾病预防控制中心,海口570203
25.08.2022
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1674-5817 |
| DOI | 10.12300/j.issn.1674-5817.2022.023 |
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| Abstract | R-332%Q95-33; 目的 观察SD大鼠血清样本放置时间对血清中K+、Na+、Ca2+、Cl-含量和pH值检测结果的影响.方法 取雌雄SD大鼠各20只,腹腔静脉采血后分离血清,样本加盖存放于2～8℃,在0h、2h、4h、6h、24h、48 h和120 h共7个时间点分别测定K+、Na+、Ca2+、Cl-含量和pH值.结果 K+、Na+含量各时间点比较差异无统计学意义(P>0.05);与0 h检测结果比较,放置48 h和120 h的Cl-含量明显升高(P<0.05),放置48 h和120 h的Ca 2+含量明显降低(P<0.05),放置24 h、48 h、120 h的pH值明显升高(P<0.05);而在6 h内这3个指标均无明显差异(P>0.05).结论 SD大鼠血清样本放置时间对血清中Ca2+、Cl-、pH检测结果有明显影响,样本应加盖贮存于2～8℃,并在6h内完成检测. |
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| AbstractList | R-332%Q95-33; 目的 观察SD大鼠血清样本放置时间对血清中K+、Na+、Ca2+、Cl-含量和pH值检测结果的影响.方法 取雌雄SD大鼠各20只,腹腔静脉采血后分离血清,样本加盖存放于2～8℃,在0h、2h、4h、6h、24h、48 h和120 h共7个时间点分别测定K+、Na+、Ca2+、Cl-含量和pH值.结果 K+、Na+含量各时间点比较差异无统计学意义(P>0.05);与0 h检测结果比较,放置48 h和120 h的Cl-含量明显升高(P<0.05),放置48 h和120 h的Ca 2+含量明显降低(P<0.05),放置24 h、48 h、120 h的pH值明显升高(P<0.05);而在6 h内这3个指标均无明显差异(P>0.05).结论 SD大鼠血清样本放置时间对血清中Ca2+、Cl-、pH检测结果有明显影响,样本应加盖贮存于2～8℃,并在6h内完成检测. |
| Author | 冯丁山 张晓昕 黄业宇 吴爱琴 王湛 苏林梁 |
| AuthorAffiliation | 海南省疾病预防控制中心,海口570203 |
| AuthorAffiliation_xml | – name: 海南省疾病预防控制中心,海口570203 |
| Author_FL | WANG Zhan HUANG Yeyu WU Aiqin FENG Dingshan SU Linliang ZHANG Xiaoxin |
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| Snippet | R-332%Q95-33; 目的 观察SD大鼠血清样本放置时间对血清中K+、Na+、Ca2+、Cl-含量和pH值检测结果的影响.方法 取雌雄SD大鼠各20只,腹腔静脉采血后分离血清,样本加盖存放于2～8℃,在0h、2h、4h、6h、24h、48 h和120... |
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| Title | 放置时间对大鼠血清样本中电解质含量及pH值的影响 |
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