利用分子检测和基因编辑快速创制籼粳杂种亲和材料

S511%Q78; [目的]利用分子检测和基因编辑技术,快速精准创制籼粳人工亲和系材料,打破籼粳杂种不育,为实现远缘杂种优势利用提供新范例.[方法]本研究以优质籼稻'黄华占'(HHZ)和优质粳稻'中嘉 8 号'(ZJ8)为测试底盘,利用分子检测技术,检测HHZ和ZJ8 中影响籼粳杂种不育的主效座位基因型,结合基因编辑技术,对影响HHZ与ZJ8 杂种F1 育性的主效座位进行基因敲除,从而快速精准创建具有高结实率的HHZ与ZJ8 籼粳杂种F1.[结果]HHZ与ZJ8 杂种F1 表现出强大的杂种优势,但其小穗育性为半不育.根据籼粳稻亚种间杂种不育已有认知,通过...

Full description

Saved in:

Bibliographic Details
Published in	华南农业大学学报 Vol. 45; no. 4; pp. 487 - 494
Main Authors	黎珠玉, 蒋永捷, 冯耀明, 梅林, 李顺媚, 郑琳, 陈乐天, 刘耀光, 谢勇尧
Format	Journal Article
Language	Chinese
Published	华南农业大学生命科学学院/亚热带农业生物资源保护与利用国家重点实验室/岭南现代农业科学与技术广东省实验室,广东广州 510642 01.07.2024
Subjects	基因编辑杂种不育 Hybrid sterility Molecular detection 杂种亲和 Hybrid compatibility 分子检测水稻 Genome editing Rice
Online Access	Get full text
ISSN	1001-411X
DOI	10.7671/j.issn.1001-411X.202403003

Cover

Abstract	S511%Q78; [目的]利用分子检测和基因编辑技术,快速精准创制籼粳人工亲和系材料,打破籼粳杂种不育,为实现远缘杂种优势利用提供新范例.[方法]本研究以优质籼稻'黄华占'(HHZ)和优质粳稻'中嘉 8 号'(ZJ8)为测试底盘,利用分子检测技术,检测HHZ和ZJ8 中影响籼粳杂种不育的主效座位基因型,结合基因编辑技术,对影响HHZ与ZJ8 杂种F1 育性的主效座位进行基因敲除,从而快速精准创建具有高结实率的HHZ与ZJ8 籼粳杂种F1.[结果]HHZ与ZJ8 杂种F1 表现出强大的杂种优势,但其小穗育性为半不育.根据籼粳稻亚种间杂种不育已有认知,通过分子检测,发现该杂交组合存在一个已克隆的杂种不育主效座位S5.为此,本研究利用基因编辑技术,分别敲除S5 座位籼稻的杀手基因ORF5+和粳稻的帮凶基因ORF4+,与野生型相比,这些编辑材料的重要农艺性状无明显变化.但这些编辑材料分别与野生型ZJ8 和HHZ杂交获得的杂种F1 的小穗育性能够完全恢复到全可育.[结论]利用分子检测和基因编辑能实现籼粳杂交育种的理性设计,快速培育出籼粳亲和型材料,最大程度地保留籼粳稻遗传多样性,为突破籼粳杂种不育和实现籼粳杂种优势的充分利用提供了一种新模式.
AbstractList	S511%Q78; [目的]利用分子检测和基因编辑技术,快速精准创制籼粳人工亲和系材料,打破籼粳杂种不育,为实现远缘杂种优势利用提供新范例.[方法]本研究以优质籼稻'黄华占'(HHZ)和优质粳稻'中嘉 8 号'(ZJ8)为测试底盘,利用分子检测技术,检测HHZ和ZJ8 中影响籼粳杂种不育的主效座位基因型,结合基因编辑技术,对影响HHZ与ZJ8 杂种F1 育性的主效座位进行基因敲除,从而快速精准创建具有高结实率的HHZ与ZJ8 籼粳杂种F1.[结果]HHZ与ZJ8 杂种F1 表现出强大的杂种优势,但其小穗育性为半不育.根据籼粳稻亚种间杂种不育已有认知,通过分子检测,发现该杂交组合存在一个已克隆的杂种不育主效座位S5.为此,本研究利用基因编辑技术,分别敲除S5 座位籼稻的杀手基因ORF5+和粳稻的帮凶基因ORF4+,与野生型相比,这些编辑材料的重要农艺性状无明显变化.但这些编辑材料分别与野生型ZJ8 和HHZ杂交获得的杂种F1 的小穗育性能够完全恢复到全可育.[结论]利用分子检测和基因编辑能实现籼粳杂交育种的理性设计,快速培育出籼粳亲和型材料,最大程度地保留籼粳稻遗传多样性,为突破籼粳杂种不育和实现籼粳杂种优势的充分利用提供了一种新模式.
Abstract_FL	[Objective]By molecular detection and genome editing,a rapid and precise creation of artificial hybrid-compatible lines is carried out to break the indica-japonica hybrid sterility and provide a new paradigm for realizing the utilization of indica-japonica hybrid vigor.[Method]In this study,an elite indica rice variety'Huanghuazhan'(HHZ)and an elite japonica rice variety'Zhongjia 8'(ZJ8)were used as test materials.The genotypes of the major indica-japonica hybrid sterility loci in HHZ and ZJ8 were detected by molecular detection.Then,the genome editing was carried out to knock out the major hybrid sterility gene(s)conferring HHZ-ZJ8 hybrid fertility,thereby rapidly and precisely creating indica-japonica hybrid F1 with high fertility.[Result]The HHZ-ZJ8 hybrid F1 plants showed strong hybrid vigor,but exhibited spikelet semi-sterility.According to genetic mechanism of intersubspecific hybrid sterility in indica and japonica rice,molecular detection revealed that one known major locus S5 exists in the HHZ-ZJ8 hybrids.Then,genome editing was used to knockout S5-ORF5+(killer gene)in HHZ and S5-ORF4+(partner gene)in ZJ8.Compared with the wild type,several important agronomic traits of these edited materials had no obvious changes.However,the spikelet fertility of the F1 plants derived from crossing these edited materials with wild type ZJ8 or HHZ were fully restored.[Conclusion]Rational design of indica-japonica hybrid breeding can be achieved by molecular detection and genome editing,enabling rapid cultivation of indica-japonica artificial hybrid-compatible lines and maximizing the retention of genetic diversity in indica and japonica rice.This study provides a new example for breaking theindica-japonicahybrid sterility and utilizing indica-japonica hybrid vigor.
Author	刘耀光郑琳黎珠玉蒋永捷李顺媚谢勇尧陈乐天冯耀明梅林
AuthorAffiliation	华南农业大学生命科学学院/亚热带农业生物资源保护与利用国家重点实验室/岭南现代农业科学与技术广东省实验室,广东广州 510642
AuthorAffiliation_xml	– name: 华南农业大学生命科学学院/亚热带农业生物资源保护与利用国家重点实验室/岭南现代农业科学与技术广东省实验室,广东广州 510642
Author_FL	LI Zhuyu MEI Lin CHEN Letian ZHENG Lin XIE Yongyao LI Shunmei LIU Yaoguang FENG Yaoming JIANG Yongjie
Author_FL_xml	– sequence: 1 fullname: LI Zhuyu – sequence: 2 fullname: JIANG Yongjie – sequence: 3 fullname: FENG Yaoming – sequence: 4 fullname: MEI Lin – sequence: 5 fullname: LI Shunmei – sequence: 6 fullname: ZHENG Lin – sequence: 7 fullname: CHEN Letian – sequence: 8 fullname: LIU Yaoguang – sequence: 9 fullname: XIE Yongyao
Author_xml	– sequence: 1 fullname: 黎珠玉 – sequence: 2 fullname: 蒋永捷 – sequence: 3 fullname: 冯耀明 – sequence: 4 fullname: 梅林 – sequence: 5 fullname: 李顺媚 – sequence: 6 fullname: 郑琳 – sequence: 7 fullname: 陈乐天 – sequence: 8 fullname: 刘耀光 – sequence: 9 fullname: 谢勇尧
BookMark	eNo9Tz1Lw0AYvqGCtfZPuDkkvpe73CWjlPoBBRcFt5K7XLRFrmAQ61YcJKBCK1KQUio4qIiDFR1K0T_jJfovDCpODzw8n3OooFtaIbSAweaM46Wm3YhjbWMAbFGMt20HHAoEgBRQ8Z-dReU4bggAByjktiKqmuQ-u7wzyYl57KY3nfTl1FycmdHEDK6zaf_zrWfeH746I5MMTPKaPU2z8XM6PM5uzz8m41yZDrtp_2oezUTBXqzKf1hCWyvVzcqaVdtYXa8s16wYA3UtSQQmMiJMcRpRRjhRyg-kFNyjvuKhcGi-UjoecBYw4kpPOBKDZKGvVOhRUkKLv7mHgY4CvVNvtg72dd5Y39X6KGy3xc9tCuCSbyeian8
ClassificationCodes	S511%Q78
ContentType	Journal Article
Copyright	Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml	– notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID	2B. 4A8 92I 93N PSX TCJ
DOI	10.7671/j.issn.1001-411X.202403003
DatabaseName	Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod	fulltext_linktorsrc
Discipline	Agriculture
DocumentTitle_FL	Rapidly creating indica-japonica hybrid compatibility materials by molecular detection and genome editing
EndPage	494
ExternalDocumentID	hnnydxxb202404005
GrantInformation_xml	– fundername: (国家自然科学基金); (国家自然科学基金); (广东省基础与应用基础研究基金自然科学基金杰出青年项目); (岭南现代农业实验室科研项目) funderid: (国家自然科学基金); (国家自然科学基金); (广东省基础与应用基础研究基金自然科学基金杰出青年项目); (岭南现代农业实验室科研项目)
GroupedDBID	-04 2B. 4A8 5XA 5XE 92G 92I 93N ABJNI ACGFS ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CW9 DYU ECGQY OZF PSX TCJ TGD U1G U5N
ID	FETCH-LOGICAL-s1045-c3b13cf36e74f46373ee9accb7849e7db24411c28076a635c8b2c10c6d9eed843
ISSN	1001-411X
IngestDate	Thu May 29 04:04:40 EDT 2025
IsPeerReviewed	false
IsScholarly	false
Issue	4
Keywords	基因编辑杂种不育 Hybrid sterility Molecular detection 杂种亲和 Hybrid compatibility 分子检测水稻 Genome editing Rice
Language	Chinese
LinkModel	OpenURL
MergedId	FETCHMERGED-LOGICAL-s1045-c3b13cf36e74f46373ee9accb7849e7db24411c28076a635c8b2c10c6d9eed843
PageCount	8
ParticipantIDs	wanfang_journals_hnnydxxb202404005
PublicationCentury	2000
PublicationDate	2024-07-01
PublicationDateYYYYMMDD	2024-07-01
PublicationDate_xml	– month: 07 year: 2024 text: 2024-07-01 day: 01
PublicationDecade	2020
PublicationTitle	华南农业大学学报
PublicationTitle_FL	Journal of South China Agricultural University
PublicationYear	2024
Publisher	华南农业大学生命科学学院/亚热带农业生物资源保护与利用国家重点实验室/岭南现代农业科学与技术广东省实验室,广东广州 510642
Publisher_xml	– name: 华南农业大学生命科学学院/亚热带农业生物资源保护与利用国家重点实验室/岭南现代农业科学与技术广东省实验室,广东广州 510642
SSID	ssib002040671 ssib051370473 ssib002806938 ssib036437894 ssib005319396 ssj0048704
Score	1.9607215
Snippet	S511%Q78; [目的]利用分子检测和基因编辑技术,快速精准创制籼粳人工亲和系材料,打破籼粳杂种不育,为实现远缘杂种优势利用提供新范例.[方法]本研究以优质籼稻'黄华占'(HHZ)和优质粳稻'中嘉 8 号'(ZJ8)为测试底盘,利用分子检测技术,检测HHZ和ZJ8...
SourceID	wanfang
SourceType	Aggregation Database
StartPage	487
Title	利用分子检测和基因编辑快速创制籼粳杂种亲和材料
URI	https://d.wanfangdata.com.cn/periodical/hnnydxxb202404005
Volume	45
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LaxRBEB5CctGD-MQ3IvZF2bgz09OPY_fuLEHQkwE9hd3Z2eS0QowQcwoeJKBCFAmISAQPKuLBiB5C0D_j7Oq_sKq6M7MkUaKXodJdXVVdX2W6urenOwguZQiyjlSt1467NS5EUmtLKWvdKMt6iday08OJ4vUbYmqaX7uV3BqbuDyya-neQmcyW9rzu5L_QRXKAFf8SvYfkC2FQgHQgC88AWF47gtjliZMKWY0SyXTnBnlS5RAwjSZrrNUMBPjhgYgLNRarNIRUw0iWswaIiwzdZRjG0xDc8VsynSIVbbFDLTStCui5VVo6wkrqFWIDZGImI1Rl24yFWGJkUw1WcpREdRW2onHWQga3Q1G24kyCQcJqSe0JALYGiQKDCCzDUf5rrNGjBCCKcNMsh1QaL61JA_Mafm-wp9KVyyKLLPkqTrqQClNZmXFQjaYFvKCN5xXNdBpxQL1ICWhGnChHF1YiXi5Cdf9K_xvPwlvBwaAZJve0Q6wnb7Q4Fw0Kmp5GFAm9D5GBgRYef6dekstwKwpzhTFECcXGQKPY3wAkN7j3Df3PdoVnRA3lpSalEIHosrFh8RwsdpXgeegyhgfplACkiPqL8SQM9u7i2C0zhKLsb5nR3Y6pzSSzPZANgjaBM2wre3mFNbwVOFfbWvs1bAsAQKEpFdgcBJ8ZKkdDyer8ZAudCpzBXf0qX8n8pGBn_u00eWQ3F3cvTM9kUK69AQVTJYKJjH6YLCtx1VSVm6Vnev373cXFzvEw-m45olISrcl5fZ0lWpD5ehRlbhjQo-MrTjWxtXRmjH-YK-qsTgJY1nnssxioT909WpppDuwGXtw9c_205eP_V67PzuSpN88HBzys-sLxr0qjwRjS3NHg4Nmdt6fMJQfC9Ji5f3w-bti5WHxcXXwZnnw5VHx7HGxvlm8fD3cWvv57Wnx_cOv5fVi5WWx8nX4aWu48Xnw6sHw7ZMfmxvAOXi1Olh7cTyYbqU3G1M1f5dM7W4Is9ZaFnfCOOvFIpe8x0Us4zzX7SzrSMV1LrsdmOaEYYZng4k2TMIy1YmysJ6JroZZhOLxiWC8f6efnwwu9DRX3bbgCtJc3gUv4hKOiHKR5AnMBvWp4KJ3wowfK-7O7ELx9H6YzgQHqhfT2WB8Yf5efg7mQAud87R2eJ5C4Dc8efeq
linkProvider	CAB International
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%88%A9%E7%94%A8%E5%88%86%E5%AD%90%E6%A3%80%E6%B5%8B%E5%92%8C%E5%9F%BA%E5%9B%A0%E7%BC%96%E8%BE%91%E5%BF%AB%E9%80%9F%E5%88%9B%E5%88%B6%E7%B1%BC%E7%B2%B3%E6%9D%82%E7%A7%8D%E4%BA%B2%E5%92%8C%E6%9D%90%E6%96%99&rft.jtitle=%E5%8D%8E%E5%8D%97%E5%86%9C%E4%B8%9A%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5&rft.au=%E9%BB%8E%E7%8F%A0%E7%8E%89&rft.au=%E8%92%8B%E6%B0%B8%E6%8D%B7&rft.au=%E5%86%AF%E8%80%80%E6%98%8E&rft.au=%E6%A2%85%E6%9E%97&rft.date=2024-07-01&rft.pub=%E5%8D%8E%E5%8D%97%E5%86%9C%E4%B8%9A%E5%A4%A7%E5%AD%A6%E7%94%9F%E5%91%BD%E7%A7%91%E5%AD%A6%E5%AD%A6%E9%99%A2%2F%E4%BA%9A%E7%83%AD%E5%B8%A6%E5%86%9C%E4%B8%9A%E7%94%9F%E7%89%A9%E8%B5%84%E6%BA%90%E4%BF%9D%E6%8A%A4%E4%B8%8E%E5%88%A9%E7%94%A8%E5%9B%BD%E5%AE%B6%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2F%E5%B2%AD%E5%8D%97%E7%8E%B0%E4%BB%A3%E5%86%9C%E4%B8%9A%E7%A7%91%E5%AD%A6%E4%B8%8E%E6%8A%80%E6%9C%AF%E5%B9%BF%E4%B8%9C%E7%9C%81%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E5%B9%BF%E4%B8%9C%E5%B9%BF%E5%B7%9E+510642&rft.issn=1001-411X&rft.volume=45&rft.issue=4&rft.spage=487&rft.epage=494&rft_id=info:doi/10.7671%2Fj.issn.1001-411X.202403003&rft.externalDocID=hnnydxxb202404005
thumbnail_s	http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fhnnydxxb%2Fhnnydxxb.jpg