Expression and Purification of Serine/Arginine-Rich Splicing Factor 1 from Escherichia coli Expression System

• Published in: 东华大学学报（英文版） Vol. 39; no. 5; pp. 441 - 445
• Main Authors: ZHANG Minmin, ZHANG Yunlong, CHEN Ting, LU Changrui
• Format: Journal Article
• Language: English
• Published: College of Biological Science and Medical Engineering,Donghua University,Shanghai 201620,China 31.10.2022
• Subjects: protein purification; SUMO; serine/arginine-rich splicing factor 1(SRSF1); bioinformatics; chromatography; SUMO protease

Q71; Serine/arginine-rich splicing factor 1 (SRSF1), as a prototype member of the highly conserved serine/arginine family of RNA binding proteins, plays an important role in mRNA alternative splicing, stabilization, nuclear export, and translation. Here, the expression system was established to puri...