35份狗牙根种质材料指纹图谱构建及染色体倍性鉴定
S688.4; [目的]通过鉴定来自不同地区的35份狗牙根(Cynodon spp.)种质资源,为其种质资源的开发与利用提供理论基础.[方法]以29份狗牙根优良种质材料为研究对象,以6份源自国外的优异品种为对照,利用流式细胞术(FCM)检测染色体倍性,通过相关序列扩增多态性(SRAP)和简单重复序列(SSR)分子标记技术进行遗传多样性分析并构建DNA指纹图谱.[结果]共筛选出10对SRAP引物,扩增出155条清晰的谱带,其中多态性条带有132条,多态性比率为85.16％;筛选出10对SSR引物,扩增出 166条清晰的谱带,多态性条带有144条,多态性比率为90.00％;35份狗牙根材料的遗传相...
Saved in:
| Published in | 南京农业大学学报 Vol. 46; no. 1; pp. 42 - 54 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
南京农业大学草业学院,江苏南京210095
2023
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-2030 |
| DOI | 10.7685/jnau.202201035 |
Cover
| Abstract | S688.4; [目的]通过鉴定来自不同地区的35份狗牙根(Cynodon spp.)种质资源,为其种质资源的开发与利用提供理论基础.[方法]以29份狗牙根优良种质材料为研究对象,以6份源自国外的优异品种为对照,利用流式细胞术(FCM)检测染色体倍性,通过相关序列扩增多态性(SRAP)和简单重复序列(SSR)分子标记技术进行遗传多样性分析并构建DNA指纹图谱.[结果]共筛选出10对SRAP引物,扩增出155条清晰的谱带,其中多态性条带有132条,多态性比率为85.16％;筛选出10对SSR引物,扩增出 166条清晰的谱带,多态性条带有144条,多态性比率为90.00％;35份狗牙根材料的遗传相似系数(GS)为0.524～0.927,变幅为0.403,表明这些材料之间存在丰富的遗传变异.基于GS进行聚类分析,在GS为0.699处,将35份狗牙根材料分为4类.利用7对SRAP引物和5对SSR引物分别构建35份狗牙根材料的DNA指纹图谱.通过FCM鉴定出各自的染色体倍性,其中二倍体1份、三倍体28份、四倍体6份.[结论]应用FCM、SSR和SRAP等技术能够充分揭示狗牙根不同种质材料间存在的遗传差异,可用于狗牙根种质资源的遗传多样性分析、种质鉴定等研究工作,为后续狗牙根新品种选育奠定基础. |
|---|---|
| AbstractList | S688.4; [目的]通过鉴定来自不同地区的35份狗牙根(Cynodon spp.)种质资源,为其种质资源的开发与利用提供理论基础.[方法]以29份狗牙根优良种质材料为研究对象,以6份源自国外的优异品种为对照,利用流式细胞术(FCM)检测染色体倍性,通过相关序列扩增多态性(SRAP)和简单重复序列(SSR)分子标记技术进行遗传多样性分析并构建DNA指纹图谱.[结果]共筛选出10对SRAP引物,扩增出155条清晰的谱带,其中多态性条带有132条,多态性比率为85.16％;筛选出10对SSR引物,扩增出 166条清晰的谱带,多态性条带有144条,多态性比率为90.00％;35份狗牙根材料的遗传相似系数(GS)为0.524～0.927,变幅为0.403,表明这些材料之间存在丰富的遗传变异.基于GS进行聚类分析,在GS为0.699处,将35份狗牙根材料分为4类.利用7对SRAP引物和5对SSR引物分别构建35份狗牙根材料的DNA指纹图谱.通过FCM鉴定出各自的染色体倍性,其中二倍体1份、三倍体28份、四倍体6份.[结论]应用FCM、SSR和SRAP等技术能够充分揭示狗牙根不同种质材料间存在的遗传差异,可用于狗牙根种质资源的遗传多样性分析、种质鉴定等研究工作,为后续狗牙根新品种选育奠定基础. |
| Author | 何法慧 杨志民 于景金 左倩倩 |
| AuthorAffiliation | 南京农业大学草业学院,江苏南京210095 |
| AuthorAffiliation_xml | – name: 南京农业大学草业学院,江苏南京210095 |
| Author_FL | HE Fahui YU Jingjin YANG Zhimin ZUO Qianqian |
| Author_FL_xml | – sequence: 1 fullname: HE Fahui – sequence: 2 fullname: ZUO Qianqian – sequence: 3 fullname: YU Jingjin – sequence: 4 fullname: YANG Zhimin |
| Author_xml | – sequence: 1 fullname: 何法慧 – sequence: 2 fullname: 左倩倩 – sequence: 3 fullname: 于景金 – sequence: 4 fullname: 杨志民 |
| BookMark | eNotjz1Lw0AYgG-oYK1d_Q8Oqe_d5b1LRqmfUHDpXi5JIwa5gqFYtw6CRC0KBaWKH1udRKVLW-qvaS7xX1jQ6dmeh2eFFHRLNwlZo1CRwsGNSKt2hQFjQIFjgRQpAFgMOCyTchwfeYCUS0BwimSL43w6y67us2RgXsfZsJeP3szTrbkbmOuLbDJOH7_zj0_zfJ5OJ-nNpXnp58nXfNZPuz3THf4ko_T9YZUsheo4bpb_WSL1ne16dc-qHezuVzdrVkzBRotyKgLBuKccGzn4LkreDD2FVDHhitARwvVdIWloM9cOfSG9QCJFn3JUruQlsv6nPVU6VPqwEbXaJ3oRbOhInwWdjreY5otpQP4L9Kpkug |
| ClassificationCodes | S688.4 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.7685/jnau.202201035 |
| DatabaseName | Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Agriculture |
| DocumentTitle_FL | Fingerprint construction and chromosome ploidy identification of 35 germplasms in bermudagrass |
| EndPage | 54 |
| ExternalDocumentID | njnydxxb202301005 |
| GrantInformation_xml | – fundername: (江苏省林业科技创新与推广项目); (科技部科技基础资源调查专项) funderid: (江苏省林业科技创新与推广项目); (科技部科技基础资源调查专项) |
| GroupedDBID | -04 123 2B. 4A8 92G 92I 93N ABDBF ABJNI ACGFS ACUHS ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CW9 DYU EOJEC ESX OBODZ OZF PSX TCJ TGD U1G U5N UY8 |
| ID | FETCH-LOGICAL-s1045-1316d623ba84530c9573efba51a2696f8669c9671f4294fc67bd7515c135a973 |
| ISSN | 1000-2030 |
| IngestDate | Thu May 29 04:08:26 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | SSR SRAP 狗牙根 DNA指纹图谱 染色体倍性 分子标记 流式细胞术 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-s1045-1316d623ba84530c9573efba51a2696f8669c9671f4294fc67bd7515c135a973 |
| PageCount | 13 |
| ParticipantIDs | wanfang_journals_njnydxxb202301005 |
| PublicationCentury | 2000 |
| PublicationDate | 2023 |
| PublicationDateYYYYMMDD | 2023-01-01 |
| PublicationDate_xml | – year: 2023 text: 2023 |
| PublicationDecade | 2020 |
| PublicationTitle | 南京农业大学学报 |
| PublicationTitle_FL | Journal of Nanjing Agricultural University |
| PublicationYear | 2023 |
| Publisher | 南京农业大学草业学院,江苏南京210095 |
| Publisher_xml | – name: 南京农业大学草业学院,江苏南京210095 |
| SSID | ssib051370508 ssj0041892 ssib001101132 ssib002263529 |
| Score | 2.3239698 |
| Snippet | S688.4; [目的]通过鉴定来自不同地区的35份狗牙根(Cynodon... |
| SourceID | wanfang |
| SourceType | Aggregation Database |
| StartPage | 42 |
| Title | 35份狗牙根种质材料指纹图谱构建及染色体倍性鉴定 |
| URI | https://d.wanfangdata.com.cn/periodical/njnydxxb202301005 |
| Volume | 46 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NT9VAEG8QL3owfkb8CjHusdhtu1_H7Xt9IR48QYIn0va1GA414SNBThxMDCrRhESDxo8bejFquADBv4bXh_-FM9uF9wAPyKWZ7P52dman251pd6eOcy9I8kxm7dTNeS7ckBbUTSEKc3nGwTv2ZFG0zS7fh3x0PHwwwSYGznzr27U0P5eOZIv_PFdyGqtCGdgVT8n-h2UPmEIB0GBfuIKF4XoiGweMxCGJIhI1SSyIjIgShlBEKRJzoj0SKSzRUAgYSaKQaIlVqkmUZwhuwbJBpGkeadOKEQWcY9MK-FADjokMsQo71UjIFpHaVLWIChAMvUe-EaxpShjupsDeORIgSawMxvDRMVG630M2-KZRJMQudMOUAP-GKZGANw1DwwqIJtG8j-Aoj2b7d9K-IAxrosASEuCiBwF9hOWCIqoD4hAXTWRsNFVEt4wWgijag5hBxdEFdi2jAceRsxD7aqU-92ymwWlVldgKRvAQpq4yhtc-TBzTOUWrID5COx3rDgJxr_75qF2XMAGA79lPWHbhsu9u-ydovQrV-cqsP1Pn6D66UkKUiUlFpstkfsTH09bUq9PGHMk-Xk6XT9sLCykOD2AwW_BZXwiKu2ebj8Z7njc82ml_JjxMctTzvBkNhMe8gw98IZWq3ohg9arzqaJU9w_LZA7TlUVSTvX5fWMXnQs2YBvW9ey75AwsPr7snNdTMzZpTX7FaQZsd3un-_Jdd3mt-rLZXV_Z2_hafXxTvV2rXj3vbm12Pvze-_Gz-vSss73Vef2i-ry6t_xrd2e1s7RSLa3_Wd7ofH9_1RlrxWONUdf-ncSdpRAHuTSgvA3BQ5rIkAVeppgI8iJNGE18rnghOVeZ4oIW4PKFRcZF2hYQPWQ0YIkSwTVnsHxS5ted4YTzBNMmCd9PwgJ8aCWSUAWJYAWmTvKGnLt2CCbtw2d28phdbpwEdNM5h3T9CvGWMzg3M5_fBqd6Lr1jzPkXzjeakQ |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=35%E4%BB%BD%E7%8B%97%E7%89%99%E6%A0%B9%E7%A7%8D%E8%B4%A8%E6%9D%90%E6%96%99%E6%8C%87%E7%BA%B9%E5%9B%BE%E8%B0%B1%E6%9E%84%E5%BB%BA%E5%8F%8A%E6%9F%93%E8%89%B2%E4%BD%93%E5%80%8D%E6%80%A7%E9%89%B4%E5%AE%9A&rft.jtitle=%E5%8D%97%E4%BA%AC%E5%86%9C%E4%B8%9A%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5&rft.au=%E4%BD%95%E6%B3%95%E6%85%A7&rft.au=%E5%B7%A6%E5%80%A9%E5%80%A9&rft.au=%E4%BA%8E%E6%99%AF%E9%87%91&rft.au=%E6%9D%A8%E5%BF%97%E6%B0%91&rft.date=2023&rft.pub=%E5%8D%97%E4%BA%AC%E5%86%9C%E4%B8%9A%E5%A4%A7%E5%AD%A6%E8%8D%89%E4%B8%9A%E5%AD%A6%E9%99%A2%2C%E6%B1%9F%E8%8B%8F%E5%8D%97%E4%BA%AC210095&rft.issn=1000-2030&rft.volume=46&rft.issue=1&rft.spage=42&rft.epage=54&rft_id=info:doi/10.7685%2Fjnau.202201035&rft.externalDocID=njnydxxb202301005 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fnjnydxxb%2Fnjnydxxb.jpg |