分子印迹SPR传感器用于骆驼奶粉中β-乳球蛋白的检测

O64; 结合模板分子前定位、表面印迹法和后修饰策略,在金(Au)基底上构建了分子印迹表面等离子体共振(SPR)传感器,并将其用于骆驼奶粉中β-乳球蛋白(BLG)的检测.首先在聚多巴胺(PDA)修饰的金片(PDA/Au)上,通过巯基封端的聚丙烯酸(PAA-SH)固定模板分子BLG,以多巴胺为功能单体和交联剂进行印迹;然后在非印迹部分接枝具有抗蛋白质吸附功能的部分水解聚(2-甲基-2-噁唑啉)(PMOXA-EI),洗脱模板后,得到BLG分子印迹SPR传感器.采用水接触角(WCA)研究了传感器表面亲/疏水的变化,用傅里叶变换红外光谱仪(FT-IR)、原子力显微镜(AFM)、可变角光谱椭偏仪(VAS...

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Published in功能高分子学报 Vol. 36; no. 4; pp. 349 - 357
Main Authors 申永帅, 胡子凡, 朱良宇, 王雨晨, 何康, 王延梅
Format Journal Article
LanguageChinese
Published 中国科学技术大学高分子科学与工程系, 合肥 230026 01.08.2023
Subjects
molecular imprinting
SPR传感器
β-lactoglobulin
分子印迹
poly(2-methyl-2-oxazoline)
多巴胺
β-乳球蛋白
dopamine
聚(2-甲基-2-噁唑啉)
SPR sensor
Online AccessGet full text
ISSN1008-9357
DOI10.14133/j.cnki.1008-9357.20230321001

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Abstract O64; 结合模板分子前定位、表面印迹法和后修饰策略,在金(Au)基底上构建了分子印迹表面等离子体共振(SPR)传感器,并将其用于骆驼奶粉中β-乳球蛋白(BLG)的检测.首先在聚多巴胺(PDA)修饰的金片(PDA/Au)上,通过巯基封端的聚丙烯酸(PAA-SH)固定模板分子BLG,以多巴胺为功能单体和交联剂进行印迹;然后在非印迹部分接枝具有抗蛋白质吸附功能的部分水解聚(2-甲基-2-噁唑啉)(PMOXA-EI),洗脱模板后,得到BLG分子印迹SPR传感器.采用水接触角(WCA)研究了传感器表面亲/疏水的变化,用傅里叶变换红外光谱仪(FT-IR)、原子力显微镜(AFM)、可变角光谱椭偏仪(VASE)对其进行表征.结果表明,该传感器对 0.21～10 μg/mL的BLG具有良好的线性关系,检测限为 0.12 μg/mL,能快速灵敏地检测出骆驼奶粉中BLG的质量浓度,回收率为100.2%～100.7%.
AbstractList O64; 结合模板分子前定位、表面印迹法和后修饰策略,在金(Au)基底上构建了分子印迹表面等离子体共振(SPR)传感器,并将其用于骆驼奶粉中β-乳球蛋白(BLG)的检测.首先在聚多巴胺(PDA)修饰的金片(PDA/Au)上,通过巯基封端的聚丙烯酸(PAA-SH)固定模板分子BLG,以多巴胺为功能单体和交联剂进行印迹;然后在非印迹部分接枝具有抗蛋白质吸附功能的部分水解聚(2-甲基-2-噁唑啉)(PMOXA-EI),洗脱模板后,得到BLG分子印迹SPR传感器.采用水接触角(WCA)研究了传感器表面亲/疏水的变化,用傅里叶变换红外光谱仪(FT-IR)、原子力显微镜(AFM)、可变角光谱椭偏仪(VASE)对其进行表征.结果表明,该传感器对 0.21～10 μg/mL的BLG具有良好的线性关系,检测限为 0.12 μg/mL,能快速灵敏地检测出骆驼奶粉中BLG的质量浓度,回收率为100.2%～100.7%.
Author 朱良宇
何康
申永帅
胡子凡
王延梅
王雨晨
AuthorAffiliation 中国科学技术大学高分子科学与工程系, 合肥 230026
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WANG Yanmei
WANG Yuchen
ZHU Liangyu
HU Zifan
HE Kang
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Keywords molecular imprinting
SPR传感器
β-lactoglobulin
分子印迹
poly(2-methyl-2-oxazoline)
多巴胺
β-乳球蛋白
dopamine
聚(2-甲基-2-噁唑啉)
SPR sensor
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Title 分子印迹SPR传感器用于骆驼奶粉中β-乳球蛋白的检测
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