两种方法制备的蛹虫草多糖特性及体外生物活性对比分析

TQ281; 本研究以蛹虫草发酵液为材料,采用壳聚糖絮凝法和水提醇沉法分别制备了絮凝多糖(XDT)和醇沉多糖(CDT),并对两种方法制备所得多糖的得率、各组分含量、平均粒径和复溶性进行了对比分析,利用扫描电镜、傅里叶红外光谱和刚果红实验对其微观结构、官能团和空间构象进行表征,利用DPPH、超氧阴离子和羟自由基清除能力评价其抗氧化活性,以α-葡萄糖苷酶抑制率评价其降血糖活性,以HepG2细胞增殖抑制率评价其抗肿瘤活性.结果表明:壳聚糖絮凝法制备多糖得率为 3.58%,是醇沉法的 1.91倍;两种多糖在扫描电镜下的微观结构存在差异,在红外光谱下吸收图谱相似,但XDT比CDT具有更多的三螺旋结构;X...

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Published in食品工业科技 Vol. 44; no. 18; pp. 76 - 83
Main Authors 顾丹丹, 侯静宇, 张金秀, 王立安
Format Journal Article
LanguageChinese
Published 石家庄学院化工学院,河北石家庄 050035%河北师范大学生命科学学院,河北石家庄 050024 01.09.2023
Subjects
结构特征
水提醇沉法
壳聚糖絮凝法
biological activity
polysaccharide
Cordyceps militaris
structural characteristics
多糖
chitosan flocculation method
蛹虫草
water extraction and alcohol precipitation
生物活性
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Abstract TQ281; 本研究以蛹虫草发酵液为材料,采用壳聚糖絮凝法和水提醇沉法分别制备了絮凝多糖(XDT)和醇沉多糖(CDT),并对两种方法制备所得多糖的得率、各组分含量、平均粒径和复溶性进行了对比分析,利用扫描电镜、傅里叶红外光谱和刚果红实验对其微观结构、官能团和空间构象进行表征,利用DPPH、超氧阴离子和羟自由基清除能力评价其抗氧化活性,以α-葡萄糖苷酶抑制率评价其降血糖活性,以HepG2细胞增殖抑制率评价其抗肿瘤活性.结果表明:壳聚糖絮凝法制备多糖得率为 3.58%,是醇沉法的 1.91倍;两种多糖在扫描电镜下的微观结构存在差异,在红外光谱下吸收图谱相似,但XDT比CDT具有更多的三螺旋结构;XDT的平均粒径更小,复溶速度更快;体外抗氧化实验表明,在相同浓度下,对DPPH、超氧阴离子和羟自由基的清除能力,均表现为XDT＞CDT;体外抑制α-葡萄糖苷酶活性实验表明,低浓度时,两种多糖的抑制率没有显著性差异,但随着浓度增加,在相同条件下,XDT的抑制活性明显强于CDT;体外抑制肿瘤细胞增殖能力实验表明,在相同处理浓度下,XDT对HepG2 细胞的增殖抑制作用优于CDT.综上,壳聚糖絮凝法制备的蛹虫草多糖得率更高、平均粒径更小、复溶性更佳,XDT具备较强的体外抗氧化、降血糖和抗肿瘤活性.
AbstractList TQ281; 本研究以蛹虫草发酵液为材料,采用壳聚糖絮凝法和水提醇沉法分别制备了絮凝多糖(XDT)和醇沉多糖(CDT),并对两种方法制备所得多糖的得率、各组分含量、平均粒径和复溶性进行了对比分析,利用扫描电镜、傅里叶红外光谱和刚果红实验对其微观结构、官能团和空间构象进行表征,利用DPPH、超氧阴离子和羟自由基清除能力评价其抗氧化活性,以α-葡萄糖苷酶抑制率评价其降血糖活性,以HepG2细胞增殖抑制率评价其抗肿瘤活性.结果表明:壳聚糖絮凝法制备多糖得率为 3.58%,是醇沉法的 1.91倍;两种多糖在扫描电镜下的微观结构存在差异,在红外光谱下吸收图谱相似,但XDT比CDT具有更多的三螺旋结构;XDT的平均粒径更小,复溶速度更快;体外抗氧化实验表明,在相同浓度下,对DPPH、超氧阴离子和羟自由基的清除能力,均表现为XDT＞CDT;体外抑制α-葡萄糖苷酶活性实验表明,低浓度时,两种多糖的抑制率没有显著性差异,但随着浓度增加,在相同条件下,XDT的抑制活性明显强于CDT;体外抑制肿瘤细胞增殖能力实验表明,在相同处理浓度下,XDT对HepG2 细胞的增殖抑制作用优于CDT.综上,壳聚糖絮凝法制备的蛹虫草多糖得率更高、平均粒径更小、复溶性更佳,XDT具备较强的体外抗氧化、降血糖和抗肿瘤活性.
Author 侯静宇
顾丹丹
张金秀
王立安
AuthorAffiliation 石家庄学院化工学院,河北石家庄 050035%河北师范大学生命科学学院,河北石家庄 050024
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Author_FL GU Dandan
ZHANG Jinxiu
WANG Lian
HOU Jingyu
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DocumentTitle_FL Comparative Analysis of Characteristics and in Vitro Biological Activities of Cordyceps militaris Polysaccharide Prepared by Two Methods
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Keywords 结构特征
水提醇沉法
壳聚糖絮凝法
biological activity
polysaccharide
Cordyceps militaris
structural characteristics
多糖
chitosan flocculation method
蛹虫草
water extraction and alcohol precipitation
生物活性
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