甘蔗与斑茅杂交染色体组构成特征研究

斑茅是甘蔗重要的野生种质资源,渗入斑茅血缘来提高甘蔗抗性是目前甘蔗育种的重要途径之一.对甘蔗与斑茅杂交后代进行染色体分型有利于高效利用斑茅的各种优异性状.本研究利用斑茅特异引物鉴定斑茅与甘蔗杂交高世代材料的真实性,通过荧光原位杂交对甘蔗与斑茅染色体进行分型,以探究斑茅和甘蔗染色体在子代中的遗传与分离,并分析其染色体组结构特征.结果表明,杂交群体中有30份为斑茅的真实后代,包含斑茅染色体从1～10条不等,说明其后代群体基本服从n+n的遗传方式,占整个真实后代群体的60%.斑茅与甘蔗发生染色体水平的重组约16.67%,并且斑茅与不同血缘甘蔗重组的概率趋近一致.割手密种特异探针共定位结果表明,斑茅血...

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Published in作物学报 Vol. 50; no. 3; pp. 633 - 644
Main Authors 薛丽, 李心怡, 黄勇泰, 欧财篮, 吴小青, 余泽怀, 崔泽田, 张木清, 邓祖湖, 余凡
Format Journal Article
LanguageChinese
Published 亚热带农业生物资源保护与利用国家重点实验室/广西甘蔗生物学重点实验室/广西大学农学院,广西南宁 530004%福建农林大学农学院国家甘蔗工程技术研究中心,福建福州 350002 12.03.2024
Subjects
fluorescence in situ hybridization
甘蔗
Saccharum
chromosome inheritance
染色体遗传
斑茅
荧光原位杂交
Tripidium arundinaceum
Online AccessGet full text
ISSN0496-3490
DOI10.3724/SP.J.1006.2024.34100

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Abstract 斑茅是甘蔗重要的野生种质资源,渗入斑茅血缘来提高甘蔗抗性是目前甘蔗育种的重要途径之一.对甘蔗与斑茅杂交后代进行染色体分型有利于高效利用斑茅的各种优异性状.本研究利用斑茅特异引物鉴定斑茅与甘蔗杂交高世代材料的真实性,通过荧光原位杂交对甘蔗与斑茅染色体进行分型,以探究斑茅和甘蔗染色体在子代中的遗传与分离,并分析其染色体组结构特征.结果表明,杂交群体中有30份为斑茅的真实后代,包含斑茅染色体从1～10条不等,说明其后代群体基本服从n+n的遗传方式,占整个真实后代群体的60%.斑茅与甘蔗发生染色体水平的重组约16.67%,并且斑茅与不同血缘甘蔗重组的概率趋近一致.割手密种特异探针共定位结果表明,斑茅血缘的渗入降低了近现代栽培甘蔗种中热带种血缘与重组血缘的占比,并同时提高了割手密血缘的比例.本研究分析的甘蔗与斑茅杂交后代中不同血缘染色体组的遗传及结构特点,为提高斑茅种质资源在甘蔗育种中的开发利用提供了细胞遗传学基础.
AbstractList 斑茅是甘蔗重要的野生种质资源,渗入斑茅血缘来提高甘蔗抗性是目前甘蔗育种的重要途径之一.对甘蔗与斑茅杂交后代进行染色体分型有利于高效利用斑茅的各种优异性状.本研究利用斑茅特异引物鉴定斑茅与甘蔗杂交高世代材料的真实性,通过荧光原位杂交对甘蔗与斑茅染色体进行分型,以探究斑茅和甘蔗染色体在子代中的遗传与分离,并分析其染色体组结构特征.结果表明,杂交群体中有30份为斑茅的真实后代,包含斑茅染色体从1～10条不等,说明其后代群体基本服从n+n的遗传方式,占整个真实后代群体的60%.斑茅与甘蔗发生染色体水平的重组约16.67%,并且斑茅与不同血缘甘蔗重组的概率趋近一致.割手密种特异探针共定位结果表明,斑茅血缘的渗入降低了近现代栽培甘蔗种中热带种血缘与重组血缘的占比,并同时提高了割手密血缘的比例.本研究分析的甘蔗与斑茅杂交后代中不同血缘染色体组的遗传及结构特点,为提高斑茅种质资源在甘蔗育种中的开发利用提供了细胞遗传学基础.
Abstract_FL T.arundinaceum(Tripidium arundinaceum)is an important wild germplasm resource of sugarcane.It is one of the important ways of sugarcane breeding to infiltrate its lineage to improve sugarcane resistance.Karyotyping of the hybrids between sugarcane and T.arundinaceum is beneficial for the efficient utilization of its various superior traits.In this study,we used spe-cies-specific primers for identifying the authenticity of the high generation hybrids between sugarcane and T.arundinaceum,and typed the sugarcane and T.arundinaceum chromosomes by fluorescence in situ hybridization to investigate the inheritance,seg-regation,and structural characteristics of T.arundinaceum in the offspring.These results indicated that 30 clones were true prog-eny with 1-10 T.arundinaceum chromosomes,indicating that their progeny population basically obeyed n+n mode of chromo-some inheritance that accounted for 60%in the entire true progeny population.The probability of recombination at the chromo-somal level between T.arundinaceum and sugarcane was about 16.67%,and the probability of recombination between T.arun-dinaceum and sugarcane of different lineages tended to be the same.Co-localization of S.spontaneum species-specific probes showed that infiltration of T.arundinaceum lineages reduced the proportion of S.officinarum and recombinant chromosomes in modern sugarcane cultivars,and simultaneously increased the proportion of S.spontaneum.In conclusion,analyzing the genetic and structural characteristics of different chromosome sets in the hybrids between sugarcane and T.arundinaceum provides a cytogenetic basis for improving the exploitation of T.arundinaceum germplasm resources in sugarcane breeding.
Author 欧财篮
吴小青
余凡
薛丽
李心怡
黄勇泰
余泽怀
崔泽田
邓祖湖
张木清
AuthorAffiliation 亚热带农业生物资源保护与利用国家重点实验室/广西甘蔗生物学重点实验室/广西大学农学院,广西南宁 530004%福建农林大学农学院国家甘蔗工程技术研究中心,福建福州 350002
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Author_FL OU Cai-Lan
LI Xin-Yi
ZHANG Mu-Qing
YU Fan
DENG Zu-Hu
WU Xiao-Qing
CUI Ze-Tian
HUANG Yong-Tai
XUE Li
YU Ze-Huai
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Keywords fluorescence in situ hybridization
甘蔗
Saccharum
chromosome inheritance
染色体遗传
斑茅
荧光原位杂交
Tripidium arundinaceum
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