茯苓渣多糖组成分析及体外抗癌、免疫活性研究

TS201.4; 目的:以茯苓渣为原料,通过热水浸提茯苓多糖,研究其单糖组成及抗癌、抗炎症因子及免疫活性,为开发茯苓多糖相关产品提供了理论依据.方法:利用高效液相色谱和红外光谱对水提茯苓多糖进行单糖组成分析,采用CCK-8法对水提茯苓多糖进行抗肿瘤及免疫活性研究.结果:水提茯苓多糖的多糖含量为76％,具有明显的多糖特征吸收峰,主要由葡萄糖、甘露糖、半乳糖、岩藻糖组成,其物质的量之比为1:0.107:0.079:0.018;对胃癌细胞、乳腺癌细胞、肝癌细胞均有抑制作用,对胃癌细胞的增殖抑制效果最优(IC50=1096μg/mL),对小鼠脾淋巴细胞具有促进增殖作用.结论:水提茯苓多糖具有良好的体外...

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Published in食品工业科技 Vol. 43; no. 21; pp. 381 - 387
Main Authors 关玉婷, 温思萌, 冯雪, 白云鹏, 陈瑞瑞, 沈晓勇, 冯佳宁, 常世敏, 程鑫颖
Format Journal Article
LanguageChinese
Published 河北工程大学生命科学与食品工程学院,河北邯郸 056038 01.11.2022
邯郸市天然产物与功能食品开发重点实验室,河北邯郸 056038%晨光生物科技集团邯郸有限公司,河北邯郸 056004
Subjects
抗癌活性
茯苓
结构表征
免疫活性
多糖
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2022010168

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Abstract TS201.4; 目的:以茯苓渣为原料,通过热水浸提茯苓多糖,研究其单糖组成及抗癌、抗炎症因子及免疫活性,为开发茯苓多糖相关产品提供了理论依据.方法:利用高效液相色谱和红外光谱对水提茯苓多糖进行单糖组成分析,采用CCK-8法对水提茯苓多糖进行抗肿瘤及免疫活性研究.结果:水提茯苓多糖的多糖含量为76％,具有明显的多糖特征吸收峰,主要由葡萄糖、甘露糖、半乳糖、岩藻糖组成,其物质的量之比为1:0.107:0.079:0.018;对胃癌细胞、乳腺癌细胞、肝癌细胞均有抑制作用,对胃癌细胞的增殖抑制效果最优(IC50=1096μg/mL),对小鼠脾淋巴细胞具有促进增殖作用.结论:水提茯苓多糖具有良好的体外抗癌及免疫活性.
AbstractList TS201.4; 目的:以茯苓渣为原料,通过热水浸提茯苓多糖,研究其单糖组成及抗癌、抗炎症因子及免疫活性,为开发茯苓多糖相关产品提供了理论依据.方法:利用高效液相色谱和红外光谱对水提茯苓多糖进行单糖组成分析,采用CCK-8法对水提茯苓多糖进行抗肿瘤及免疫活性研究.结果:水提茯苓多糖的多糖含量为76％,具有明显的多糖特征吸收峰,主要由葡萄糖、甘露糖、半乳糖、岩藻糖组成,其物质的量之比为1:0.107:0.079:0.018;对胃癌细胞、乳腺癌细胞、肝癌细胞均有抑制作用,对胃癌细胞的增殖抑制效果最优(IC50=1096μg/mL),对小鼠脾淋巴细胞具有促进增殖作用.结论:水提茯苓多糖具有良好的体外抗癌及免疫活性.
Author 关玉婷
温思萌
冯雪
冯佳宁
沈晓勇
陈瑞瑞
常世敏
程鑫颖
白云鹏
AuthorAffiliation 河北工程大学生命科学与食品工程学院,河北邯郸 056038;邯郸市天然产物与功能食品开发重点实验室,河北邯郸 056038%晨光生物科技集团邯郸有限公司,河北邯郸 056004
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Author_FL WEN Simeng
CHANG Shimin
CHENG Xinying
FENG Xue
FENG Jianing
BAI Yunpeng
SHEN Xiaoyong
CHEN Ruirui
GUAN Yuting
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Keywords 抗癌活性
茯苓
结构表征
免疫活性
多糖
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邯郸市天然产物与功能食品开发重点实验室,河北邯郸 056038%晨光生物科技集团邯郸有限公司,河北邯郸 056004
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Title 茯苓渣多糖组成分析及体外抗癌、免疫活性研究
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