耐酒精高产L-乳酸菌株的筛选及发酵培养基优化

TS201.3; 为提高L-乳酸产量,降低L-乳酸的生产成本,该研究经过筛选、驯化获得一株耐酒精且高产L-乳酸的菌株鼠李糖乳杆菌AK-0779.使用玉米酒糟代替部分酵母粉作为菌株AK-0779发酵培养基的氮源.在单因素实验基础上,对葡萄糖添加量、酵母粉添加量和玉米酒糟添加量进行三因素三水平响应面优化试验.结果表明,最适发酵培养基为:葡萄糖添加量9.80％,玉米酒糟添加量0.98％,酵母粉添加量1.72％,L-乳酸产量为78.91 g/L,糖酸转换率为80.52％.与酵母粉完全充当氮源产L-乳酸82.36 g/L相比,产量无显著差异,说明玉米酒糟能有效代替部分酵母粉作为发酵培养基的氮源,降低L-...

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Published in食品工业科技 Vol. 44; no. 8; pp. 135 - 143
Main Authors 肖敏敏, 邢馨月, 刘文光, 孟祥慧, 魏姗姗, 张天笑, 王玉华, 李侠
Format Journal Article
LanguageChinese
Published 吉林农业大学食品科学与工程学院,吉林长春 130118%华信检测技术有限公司,吉林长春 130000 01.08.2023
Subjects
鼠李糖乳杆菌
响应面分析
L-乳酸
玉米酒糟
发酵培养基优化
Online AccessGet full text
ISSN1002-0306
DOI10.13386/j.issn1002-0306.2022040147

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Abstract TS201.3; 为提高L-乳酸产量,降低L-乳酸的生产成本,该研究经过筛选、驯化获得一株耐酒精且高产L-乳酸的菌株鼠李糖乳杆菌AK-0779.使用玉米酒糟代替部分酵母粉作为菌株AK-0779发酵培养基的氮源.在单因素实验基础上,对葡萄糖添加量、酵母粉添加量和玉米酒糟添加量进行三因素三水平响应面优化试验.结果表明,最适发酵培养基为:葡萄糖添加量9.80％,玉米酒糟添加量0.98％,酵母粉添加量1.72％,L-乳酸产量为78.91 g/L,糖酸转换率为80.52％.与酵母粉完全充当氮源产L-乳酸82.36 g/L相比,产量无显著差异,说明玉米酒糟能有效代替部分酵母粉作为发酵培养基的氮源,降低L-乳酸生产成本.
AbstractList TS201.3; 为提高L-乳酸产量,降低L-乳酸的生产成本,该研究经过筛选、驯化获得一株耐酒精且高产L-乳酸的菌株鼠李糖乳杆菌AK-0779.使用玉米酒糟代替部分酵母粉作为菌株AK-0779发酵培养基的氮源.在单因素实验基础上,对葡萄糖添加量、酵母粉添加量和玉米酒糟添加量进行三因素三水平响应面优化试验.结果表明,最适发酵培养基为:葡萄糖添加量9.80％,玉米酒糟添加量0.98％,酵母粉添加量1.72％,L-乳酸产量为78.91 g/L,糖酸转换率为80.52％.与酵母粉完全充当氮源产L-乳酸82.36 g/L相比,产量无显著差异,说明玉米酒糟能有效代替部分酵母粉作为发酵培养基的氮源,降低L-乳酸生产成本.
Author 王玉华
邢馨月
孟祥慧
张天笑
肖敏敏
刘文光
魏姗姗
李侠
AuthorAffiliation 吉林农业大学食品科学与工程学院,吉林长春 130118%华信检测技术有限公司,吉林长春 130000
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Author_FL ZHANG Tianxiao
XIAO Minmin
LIU Wenguang
WANG Yuhua
LI Xia
XING Xinyue
MENG Xianghui
WEI Shanshan
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Keywords 鼠李糖乳杆菌
响应面分析
L-乳酸
玉米酒糟
发酵培养基优化
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Title 耐酒精高产L-乳酸菌株的筛选及发酵培养基优化
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